Strain Detail Sheet

Strain Name:
STOCK Mmp20em1Odk/Mmucd
Stock Number:
068130-UCD
Citation ID:
RRID:MMRRC_068130-UCD
Other Names:
MMP20flox

Strain Information

Gene Details

Mmp20em1Odk
Name: matrix metallopeptidase 20 (enamelysin); endonuclease-mediated mutation 1, Ophir D Klein
Synonyms: Mmp20em1Odk
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: CRISPR
Mmp20
Name: matrix metallopeptidase 20 (enamelysin)
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: CRISPR
NCBI: 30800
VEGA: 9
HGNC: HGNC:7167
Homologene: 21001
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Genetic Alterations
CRISPR/Cas-9 induced insertion of two loxP sites - one loxP site 172 base pairs upstream of ORF base position 124 in intron 1, and one loxP site 182 base pairs downstream of ORF base position 371 in intron 2 of the Mmp20 gene on Chromosome 9. In the presence of Cre recombinase, this insertion is predicted to induce the deletion of 636 base pairs: one of the loxP sites and the 602 base pairs between the loxP sites which includes ORF base positions 124-371, and prevents the transcription and translation of the Mmp20 gene.

Strain Description

Phenotype
The phenotype of homozygotes and hemizygotes has not been investigated.
Strain Development

Single-stranded template DNA and guide RNAs were microinjected into C57BL/6J embryos and implanted into foster mothers to result in gene-edited F0 mice. F0 mice with the desired mutation were mated to animals that harbored the Cre deleter allele (Tg(KRT14-cre/ERT2)1Ipc - MGI:2177426) and the mTmG reporter allele (Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo - MGI:3716464) of uncertain genetic background (“K14-CreER; mTmG” in PMID:11034212 and 17868096) to produce the F1 generation. F1 animals that did not harbor the K14-CreER allele were crossed with B6J(FVB)-congenic beta-actin cre mice (Tmem163Tg(ACTB-cre)2Mrt - MGI:2176050) to test if the floxed allele can recombine successfully. Cre-negative F2 progeny were sib-mated thereafter, and the mTmG allele was bred out.

Suggested Control Mice
C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Strain Origin

Donor
Ophir Klein, M.D., University of California, San Francisco.
Additional References

Li M, Indra AK, Warot X, Brocard J, Messaddeq N, Kato S, Metzger D, Chambon P. Skin abnormalities generated by temporally controlled RXRalpha mutations in mouse epidermis. Nature. 2000 Oct 5;407(6804):633-6. doi: 10.1038/35036595. (Medline PMID: 11034212)

Muzumdar MD, Tasic B, Miyamichi K, Li L, Luo L. A global double-fluorescent Cre reporter mouse. Genesis. 2007 Sep;45(9):593-605. doi: 10.1002/dvg.20335. (Medline PMID: 17868096)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Other

Breeding

MMRRC Breeding System
Backcross
Generation
N2 (C57BL/6J), F3
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes N/A
Homozygotes are fertile: Yes Yes N/A
Heterozygotes are fertile: Yes Yes N/A
Age Reproductive Decline: Undetermined Undetermined
Bred to Homozygosity
Yes
Average litter size
4 to 6
Recommended wean age
3 weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.