Strain Name:
C57BL/6N-Ppp2r5aGt(IST13127C10)Tigm/RcseMmucd
Stock Number:
068161-UCD
Citation ID:
RRID:MMRRC_068161-UCD
Other Names:
B56a hypomorph (Ppp2r5a-hm)

Strain Information

Ppp2r5aGt(IST13127C10)Tigm
Name: protein phosphatase 2, regulatory subunit B', alpha; gene trap IST13127C10, Texas A&M Institute for Genomic Medicine
Synonyms: B56alphahm
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Gene Trap
Ppp2r5a
Name: protein phosphatase 2, regulatory subunit B', alpha
Type: Gene
Species: Mouse
Chromosome: 1
Alteration at locus: Gene Trap
NCBI: 226849
HGNC: HGNC:9309
Homologene: 55961
Genetic Alterations
B56a (Ppp2r5a) knockout made from an ES line by TIGM (IST13127C10)
ES Cell Line
Lex3.13 derived from C57BL/6N
Phenotype
B56a deficiency in the whole body results in spontaneous skin lesion formation with hyperproliferation of the epidermis, hair follicles, and sebaceous glands.
MeSH Terms
  • Animals
  • Mice
  • Mice, Transgenic
  • Neoplasms, Experimental/enzymology
  • Neoplasms, Experimental/pathology
  • Neoplastic Stem Cells/pathology
  • Protein Phosphatase 2/metabolism
Strain Development
The strain was developed from embryonic stem (ES) cell clones carrying a gene trap vector in the Ppp2ra (B56α) gene obtained from the Texas A&M Institute for Genomic Medicine (TIGM).
Suggested Control Mice
C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cancer
  • Dermatology
  • Immunology and Inflammation
Donor
Rosalie Sears, Ph.D., Oregon Health and Science University.
Primary Reference

Janghorban M, Langer EM, Wang X, Zachman D, Daniel CJ, Hooper J, Fleming WH, Agarwal A, Sears RC. The tumor suppressor phosphatase PP2A-B56α regulates stemness and promotes the initiation of malignancies in a novel murine model. PLoS One. 2017 Nov 30;12(11):e0188910. doi: 10.1371/journal.pone.0188910. (Medline PMID: 29190822)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N0, F?
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068161-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / $8,533.74
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.