Strain Name:
STOCK Adam17em1Bbl/Mmjax
Stock Number:
068162-JAX
Citation ID:
RRID:MMRRC_068162-JAX
Other Names:
Adam17-delta cyto

Gene Information

Adam17em1Bbl
Name: a disintegrin and metallopeptidase domain 17; endonuclease-mediated mutation1, Carl Blobel
Synonyms: Adam17deltacyto
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: CRISPR
Adam17
Name: a disintegrin and metallopeptidase domain 17
Synonyms: Tace, CD156b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: CRISPR
NCBI: 11491
HGNC: HGNC:195
Homologene: 2395
Genetic Alterations
Targeted truncation of the cytoplasmic domain of ADAM17
Genotype Determination
ES Cell Line
Not applicable
Phenotype
These mice are viable and fertile and have a normal life span. However, they have curly whiskers and slightly wavy fur, characteristic of a hylomorphic mutation in the ADAM17/EGFR signaling pathway.
Strain Development

CRISPR/Cas9 in ES cells derived from B6(Cg)-Tyrc-2J/J (RRID:IMSR_JAX:000058) followed by implantation in B6(Cg)-Tyrc-2J/J blastocysts. Chimeras bred to B6(Cg)-Tyrc-2J/J. Per PMID:33957124 and donor correspondence, the line also carries some "129SvJ" genetic background components, which were introduced to make the line hardy, breed well, and are generally easier to work with.

Suggested Control Mice
Wild-type littermate controls from het/het matings
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Immunology and Inflammation
  • Models for Human Disease
  • Research Tools
Donor
Carl Blobel, M.D., Hospital for Special Surgery at Weill Cornell.
Primary Reference

Lora J, Weskamp G, Li TM, Maretzky T, Shola DTN, Monette S, Lichtenthaler SF, Lu TT, Yang C, Blobel CP. Targeted truncation of the ADAM17 cytoplasmic domain in mice results in protein destabilization and a hypomorphic phenotype. J Biol Chem. 2021 May 3:100733. doi: 10.1016/j.jbc.2021.100733. Epub ahead of print. (Medline PMID: 33957124)

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N?, F?
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Reduced Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068162-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
068162-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.