Strain Name:
C57BL/6J-Cmklr1em1Shai/Mmnc
Stock Number:
068218-UNC
Citation ID:
RRID:MMRRC_068218-UNC
Other Names:
ERV1/ChemR23 knockout

Strain Information

Cmklr1em1Shai
Name: chemerin chemokine-like receptor 1; endonuclease-mediated mutation 1, Saame Raza Shaikh
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: CRISPR
Cmklr1
Name: chemerin chemokine-like receptor 1
Synonyms: Gpcr27, ChemR23
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: CRISPR
NCBI: 14747
HGNC: HGNC:2121
Homologene: 129967
Genetic Alterations
CRISPR/Cas9-mediated genome editing targeting the Cmklr1 (ERV1/ChemR23) gene to remove all coding sequence downstream of exon 2.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    RvE1 treatment improved hyperinsulinemia and hyperglycemia of inbred mice in a manner that is dependent on the ERV1/ChemR23 receptor.
    MeSH Terms
    • Adipose Tissue, White/drug effects
    • Animals
    • Eicosapentaenoic Acid/analogs & derivatives
    • Eicosapentaenoic Acid/pharmacology
    • Glucose Intolerance/genetics
    • Hyperglycemia/genetics
    • Hyperglycemia/prevention & control
    • Hyperinsulinism/genetics
    • Hyperinsulinism/prevention & control
    • Liver/drug effects
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Obesity/genetics
    • Polymorphism, Single Nucleotide/genetics
    • Receptors, Chemokine/genetics
    • Receptors, G-Protein-Coupled/genetics
    Strain Development
    The ERV1/ChemR23 mutant mice were generated by CRISPR/Cas9-mediated genome editing. C57BL/6J zygotes were electroporated with Cas9 protein, guide RNA, and donor oligonucleotide prior to implantation into recipient pseudopregnant females. The resulting pups were screened by PCR for the presence of a deletion allele. The founder was mated to wild-type C57BL/6J females to establish a colony with the deletion allele.
    Suggested Control Mice
    C57BL/6J littermate controls
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    • Metabolism
    • Obesity
    Donor
    Saame Raza Shaikh, Ph.D., Gillings School of Global Public Health and School of Medicine, UNC.
    Primary Reference

    Pal A, Al-Shaer AE, Guesdon W, Torres MJ, Armstrong M, Quinn K, Davis T, Reisdorph N, Neufer PD, Spangenburg EE, Carroll I, Bazinet RP, Halade GV, Clària J, Shaikh SR. Resolvin E1 derived from eicosapentaenoic acid prevents hyperinsulinemia and hyperglycemia in a host genetic manner. FASEB J. 2020 Aug;34(8):10640-10656. doi: 10.1096/fj.202000830R. Epub 2020 Jun 24. (Medline PMID: 32579292)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and sib-mating
    Generation
    N1 (C57BL/6J), F?
    Overall Breeding Performance
    Good
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: N/A N/A
    Age Reproductive Decline: 7 to 9 months Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    068218-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    068218-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.