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Availability & Fees Order this Strain
The genomic region encompassing and flanking exon 2 of the Arrb2 gene was isolated from a bacterial artificial chromosome library (Coriell Institute) containing genomic DNA from the 129SvJ mouse strain. The homology arms were generated by PCR amplification of regions upstream and downstream of exon 2; inserted into the pEZ-Frt-Lox-DT vector (RRID:Addgene_11736) containing a neomycin selection cassette flanked by flippase recognition target (FRT) sites. The targeting vector was injected into C57BL/6J-derived ES cells and subjected to neomycin selection. Chimeric pups were selected by coat color, crossed with C57BL/6J (RRID:IMSR_JAX:000664), and screened by PCR. Heterozygous floxed animals were crossed to B6N.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/J (RRID:IMSR_JAX:016226) to remove the neoR selection cassette and bred to homozygosity. Subsequently maintained by mating with C57BL/6J and sib-mating.
Note: PMID:27911814 states that the ES cells had 129S4 genetic background components. This is not the case per donor communication.
Urs NM, Gee SM, Pack TF, McCorvy JD, Evron T, Snyder JC, Yang X, Rodriguiz RM, Borrelli E, Wetsel WC, Jin J, Roth BL, O'Donnell P, Caron MG. Distinct cortical and striatal actions of a β-arrestin-biased dopamine D2 receptor ligand reveal unique antipsychotic-like properties. Proc Natl Acad Sci U S A. 2016 Dec 13;113(50):E8178-E8186. doi: 10.1073/pnas.1614347113. Epub 2016 Dec 1. (Medline PMID: 27911814)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.