Strain Name:
Stock Number:
Citation ID:
Other Names:
IEX-1 knockout mice

Gene Information

Name: immediate early response 3; targeted mutation 1, Mei X Wu
Synonyms: IEX-1 KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Knockout
Name: immediate early response 3
Synonyms: gly96, cAMP inducible gene 3, cI-3, IEX-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
NCBI: 15937
Homologene: 2894
Genetic Alterations

A 1.5-kb region including part of exon 1, all of intron 1, and most of exon 2 was replaced with a lacZ, pgk-neo cassette via homologous recombination.

ES Cell Line
Unspecified derived from 129X1/SvJ
MeSH Terms
  • Animals
  • Aorta/drug effects
  • Aorta/metabolism
  • Aorta/physiology
  • Blood Pressure/physiology
  • Blotting, Western
  • Cells, Cultured
  • Cyclic AMP/metabolism
  • Female
  • Free Radical Scavengers/pharmacology
  • GTP-Binding Protein alpha Subunit, Gi2/genetics
  • GTP-Binding Protein alpha Subunit, Gi2/metabolism
  • Immediate-Early Proteins/genetics
  • Immediate-Early Proteins/metabolism
  • In Vitro Techniques
  • Male
  • Metalloporphyrins/pharmacology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred Strains
  • Mice, Knockout
  • Mitochondria/metabolism
  • Muscle, Smooth, Vascular/cytology
  • Muscle, Smooth, Vascular/metabolism
  • Myocytes, Smooth Muscle/cytology
  • Myocytes, Smooth Muscle/drug effects
  • Myocytes, Smooth Muscle/metabolism
  • Pertussis Toxin/pharmacology
  • Rotenone/pharmacology
  • Signal Transduction/physiology
  • Superoxides/antagonists & inhibitors
  • Superoxides/metabolism
  • Vasodilation/drug effects
Strain Development
Unspecified ES cells derived from 129X1/SvJ. Chimeras were bred to C57BL/6J, followed by backcrossing to C57BL/6J to minimally N7 and sib-mating (F?, generation data unavailable).
Suggested Control Mice
Wild-type sibling
  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Hematology
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Research Tools
Mei X. Wu, Ph.D., Harvard Medical School.
Primary Reference

Shahid M, Shen L, Seldin DC, Lu B, Ustyugova IV, Chen X, Zapol WM, Wu MX. Impaired 3',5'-cyclic adenosine monophosphate-mediated signaling in immediate early responsive gene X-1-deficient vascular smooth muscle cells. Hypertension. 2010 Oct;56(4):705-12. doi: 10.1161/HYPERTENSIONAHA.110.154880. Epub 2010 Aug 16. (Medline PMID: 20713914)

Colony and Husbandry Information

For more information about this colony's health status contact
Coat Color
MMRRC Breeding System
Backcross and sib-mating
N7 (C57BL/6J), F?
Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 10 to 12 months 7 to 9 months
Bred to Homozygosity
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at for more details.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

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