Strain Name:
B6.129X1-Atf3tm1Dron/HaiMmnc
Stock Number:
068230-UNC
Citation ID:
RRID:MMRRC_068230-UNC
Other Names:
Atf3 KO

Gene Information

Atf3tm1Dron
Name: activating transcription factor 3; targeted mutation 1, David Ron
Synonyms: ATF3-, Atf3 KO, ATF3-KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Atf3
Name: activating transcription factor 3
Synonyms: LRG-21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 11910
HGNC: HGNC:785
Homologene: 1265
Genetic Alterations
Exon B of the Atf3 gene is replaced by a neo cassette.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    Unspecified derived from 129X1/SvJ
    Phenotype
    Under non-stressed conditions, the mice breed and function/behave similarly to the wild-type counterpart. Under stressed conditions, they behave differently. The phenotypes depend on the stress conditions. As an example, breast cancer metastasis is reduced in these Atf3 knockout mice.
    MeSH Terms
    • Activating Transcription Factor 3
    • Animals
    • Apoptosis/genetics
    • Cytokines/pharmacology
    • Diabetes Mellitus/metabolism
    • Diabetes Mellitus/pathology
    • Gene Expression Regulation/drug effects
    • Glucagon/analysis
    • Insulin/analysis
    • Islets of Langerhans/chemistry
    • Islets of Langerhans/pathology
    • Mice
    • Mice, Knockout
    • Mice, Transgenic
    • Pancreas/chemistry
    • Pancreas/pathology
    • Signal Transduction
    • Stress, Physiological
    • Transcription Factors/analysis
    • Transcription Factors/genetics
    • Transcription Factors/physiology
    Strain Development
    An Atf3 genomic clone was isolated from a 129SvJ library, recombined to enable homologous recombination, and introduced into unspecified ES cells derived from 129X1/SvJ. Details on chimera selection, breeding, and development of the homozygous knockouts are unavailable. Subsequent backcrossing to the C57BL/6J background over >10 generations.
    Suggested Control Mice
    wild-type C57BL/6
    • Apoptosis
    • Cancer
    • Cardiovascular
    • Cell Biology
    • Diabetes
    • Hematology
    • Immunology and Inflammation
    • Internal/Organ
    • Metabolism
    • Models for Human Disease
    • Neurobiology
    • Obesity
    • Virology
    Donor
    David Ron, Ph.D., University of Cambridge.
    Tsonwin Hai, Ph.D., Ohio State University .
    Primary Reference

    Hartman MG, Lu D, Kim ML, Kociba GJ, Shukri T, Buteau J, Wang X, Frankel WL, Guttridge D, Prentki M, Grey ST, Ron D, Hai T. Role for activating transcription factor 3 in stress-induced beta-cell apoptosis. Mol Cell Biol. 2004 Jul;24(13):5721-32. doi: 10.1128/MCB.24.13.5721-5732.2004. (Medline PMCID: 480886)

    Wolford CC, McConoughey SJ, Jalgaonkar SP, Leon M, Merchant AS, Dominick JL, Yin X, Chang Y, Zmuda EJ, O'Toole SA, Millar EK, Roller SL, Shapiro CL, Ostrowski MC, Sutherland RL, Hai T. Transcription factor ATF3 links host adaptive response to breast cancer metastasis. J Clin Invest. 2013 Jul;123(7):2893-906. doi: 10.1172/JCI64410. Epub 2013 Jun 10. (Medline PMCID: 3696548)

    Chang YS, Jalgaonkar SP, Middleton JD, Hai T. Stress-inducible gene Atf3 in the noncancer host cells contributes to chemotherapy-exacerbated breast cancer metastasis. Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):E7159-E7168. doi: 10.1073/pnas.1700455114. Epub 2017 Aug 7. (Medline PMCID: 5576783)

    Additional References

    Wolford CC, McConoughey SJ, Jalgaonkar SP, Leon M, Merchant AS, Dominick JL, Yin X, Chang Y, Zmuda EJ, O'Toole SA, Millar EK, Roller SL, Shapiro CL, Ostrowski MC, Sutherland RL, Hai T. Transcription factor ATF3 links host adaptive response to breast cancer metastasis. J Clin Invest. 2013 Jul;123(7):2893-906. doi: 10.1172/JCI64410. Epub 2013 Jun 10. (Medline PMID: 23921126)

    Chang YS, Jalgaonkar SP, Middleton JD, Hai T. Stress-inducible gene Atf3 in the noncancer host cells contributes to chemotherapy-exacerbated breast cancer metastasis. Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):E7159-E7168. doi: 10.1073/pnas.1700455114. Epub 2017 Aug 7. (Medline PMID: 28784776)

    Middleton JD, Fehlman J, Sivakumar S, Stover DG, Hai T. Stress-Inducible Gene Atf3 Dictates a Dichotomous Macrophage Activity in Chemotherapy-Enhanced Lung Colonization. Int J Mol Sci. 2021 Jul 8;22(14):7356. doi: 10.3390/ijms22147356. (Medline PMID: 34298975)

    Colony and Husbandry Information

    Similar to wild-type C57BL/6 mice except the newborns may need 4 weeks to wean, instead of 3 weeks. Sometimes, using water bottles rather than an automatic water system may be necessary. To avoid genetic drift, backcrossing the homozygotes to the C57BL/6 mice for 2-3 generations and then re-generating the homozygous KO mice is recommended.
    Coat Color
    Black
    Eye
    Black
    MMRRC Breeding System
    Backcross and random intra-strain mating
    Generation
    N13+ (C57BL/6), F10
    Overall Breeding Performance
    Excellent
    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 4 to 6 months 4 to 6 months
    Bred to Homozygosity
    Yes
    Average litter size
    4 to 6
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    When this strain becomes available, Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sadhana Chitale.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sadhana Chitale

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.