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The L1L2_Bact_P cassette was inserted at position 59169040 of Chromosome 19 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site, and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 59165833. A "conditional-ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele. The critical exon(s) is/are thus flanked by loxP sites.
This is a floxed allele of the transcription factor Vax1, ventral anterior homeobox 1. It has a variety of neural phenotypes when crossed with specific Cre alleles but no phenotype at all without a Cre.
Conditional phenotype: Yes, various depending on Cre allele.
Vax1flox mice were generated from the KO allele with conditional potential Vax1tm1a(KOMP)Mbp obtained from KOMP (UC Davis, Knock-Out Mouse Project, www.komp.org). Vax1 knockout-first mice on C57BL/6N background were crossed with the FLP deleter strain B6;SJL-Tg(ACTFLPe)9205Dym/J (RRID:IMSR_JAX:003800) to generate a Vax1 conditional-ready KO allele. The strain was maintained on C57BL/6NHsd for many years prior to archival at the MMRRC.
Hoffmann HM, Trang C, Gong P, Kimura I, Pandolfi EC, Mellon PL. Deletion of Vax1 from Gonadotropin-Releasing Hormone (GnRH) Neurons Abolishes GnRH Expression and Leads to Hypogonadism and Infertility. J Neurosci. 2016 Mar 23;36(12):3506-18. doi: 10.1523/JNEUROSCI.2723-15.2016. (Medline PMID: 27013679)
Pandolfi EC, Breuer JA, Nguyen Huu VA, Talluri T, Nguyen D, Lee JS, Hu R, Bharti K, Skowronska-Krawczyk D, Gorman MR, Mellon PL, Hoffmann HM. The Homeodomain Transcription Factors Vax1 and Six6 Are Required for SCN Development and Function. Mol Neurobiol. 2020 Feb;57(2):1217-1232. doi: 10.1007/s12035-019-01781-9. Epub 2019 Nov 9. (Medline PMID: 31705443)
Coré N, Erni A, Hoffmann HM, Mellon PL, Saurin AJ, Beclin C, Cremer H. Stem cell regionalization during olfactory bulb neurogenesis depends on regulatory interactions between Vax1 and Pax6. Elife. 2020 Aug 7;9:e58215. doi: 10.7554/eLife.58215. (Medline PMID: 32762844)
Lavalle SN, Chou T, Hernandez J, Naing NCP, Tonsfeldt KJ, Hoffmann HM, Mellon PL. Kiss1 is differentially regulated in male and female mice by the homeodomain transcription factor VAX1. Mol Cell Endocrinol. 2021 Aug 20;534:111358. doi: 10.1016/j.mce.2021.111358. Epub 2021 Jun 4. (Medline PMID: 34098016)
Hoffmann HM, Meadows JD, Breuer JA, Yaw AM, Nguyen D, Tonsfeldt KJ, Chin AY, Devries BM, Trang C, Oosterhouse HJ, Lee JS, Doser JW, Gorman MR, Welsh DK, Mellon PL. The transcription factors SIX3 and VAX1 are required for suprachiasmatic nucleus circadian output and fertility in female mice. J Neurosci Res. 2021 Jul 2. doi: 10.1002/jnr.24864. Epub ahead of print. (Medline PMID: 34212416)
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Devora Rossi.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Devora Rossi
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.