Strain Detail Sheet

Strain Name:
C57BL/6J-Tg(tetO-Mapt/mCherry)2Isb/Mmjax
Stock Number:
068345-JAX
Citation ID:
RRID:MMRRC_068345-JAX
Other Names:
Tg(mMapt-mCherry)

Strain Information

Gene Details

Tg(tetO-Mapt*/mCherry*)2Isb
Name: transgene insertion 2, Dirk Isbrandt
Type: Allele
Species: Multi-species
Chromosome: Unknown
Mapt
Name: microtubule-associated protein tau
Synonyms: Tau, Mtapt
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 17762
HGNC: HGNC:6893
Homologene: 74962
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mCherry
Name: red fluorescent fluorophore, mCherry
Type: Gene
Species: Discosoma sp. (mushroom coral)
Chromosome:
tetO
Name: tetracycline-responsive promoter element
Type: Regulatory Region
Species: Multi-species
Chromosome:
Genetic Alterations

Random insertion of a transgenic construct where the Mapt gene is fused to the mCherry gene, coding for the MCherry fluorescent protein. Both genes are codon-optimized for expression in the mouse. The expression is driven by the p-tight tet-responsive promoter.

Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
None/Normal/Wild-type
Strain Development
The transgenic construct mMapt-mcherry was injected into C57BL/6J zygotes, and three founders were identified. Founder #2 was selected for further breeding and expansion. He was mated to C57BL/6J females and progeny were screened for the presence of the transgene. The colony was backcrossed to C57BL/6J mice (RRID:IMSR_JAX:000664) for >10 generations.
Suggested Control Mice
wild-type C57BL/6J

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Neurobiology
  • Research Tools

Strain Origin

Donor
Dirk Isbrandt, M.D., DZNE & UKK Research Group, Exp Neurophysiology, IMBN.
Primary Reference
Not ready to publish

Colony and Husbandry Information

Health Status Report

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
N10+ (C57BL/6J)
Overall Breeding Performance
Good

Reproductive Statistics

NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.