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Strain Detail Sheet

Strain Name:
STOCK Dlx6os1tm2.1Jhdk/Mmmh
Stock Number:
068496-MU
Citation ID:
RRID:MMRRC_068496-MU
Other Names:
Evf1TSjdk

Strain Information

Gene Details

Dlx6os1tm2.1Jhdk
Name: distal-less homeobox 6, opposite strand 1; targeted mutation 2.1, Jhumku D Kohtz
Synonyms: Evf1TS
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
Dlx6os1
Name: distal-less homeobox 6, opposite strand 1
Synonyms: Evf1, A230055N17Rik, mEvf-1, Shhrs, Evf-2, Evf-1, Evf2, Evf1/2, Dlx6os1, Dlx6as1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knock-In
NCBI: 320038

Additional Resources
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Genetic Alterations
In Evf1TS/TS embryonic brain, Evf1 (distal-less homeobox 6, opposite strand 1, transcript variant 2 - NR_155495.1) long, non-coding RNA transcripts are not detected. However, the ultraconserved Evf2 transcript (distal-less homeobox 6, opposite strand 1, transcript variant 3 - NR_155496.1) is truncated, retaining transcription through the Dlx5/6 ultraconserved enhancer. Gene expression and chromatin immunoprecipitation comparisons with Evf2TS/TS interneuron progenitors distinguish between non-coding RNA regions in regulating the recruitment and sequestration transcription factors critical for gene regulation and chromosome organization.
ES Cell Line
W4

Strain Description

Phenotype
The Evf1TS/+ mouse strain contains a triple polyA transcription start site inserted into exon 3 of the Dlx6os1 gene (a.k.a., Evf) preventing transcription of the Evf1 non-coding RNA (NR_155495.1), and truncating the Evf2 transcript (NR_155495.1) in order to produce only the 5' ultraconserved sequence of the Evf2 non-coding RNA. These mice are valuable for distinguishing the role of the ultraconserved region of the Evf2 RNA from the 3' end of the non-coding RNA in interneuron gene regulation. The Evf1TS and Evf2TS are unique in that these are the best-characterized knock-in mouse models (perhaps the only) to compare ultraconserved enhancer transcriptional mechanism and function in vivo.
MeSH Terms
  • Alcohol Oxidoreductases/genetics
  • Animals
  • Cell Cycle Proteins/genetics
  • Chromosomal Proteins, Non-Histone/genetics
  • Conserved Sequence
  • Enhancer Elements, Genetic/genetics
  • Gene Expression Regulation, Developmental/genetics
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology
  • Interneurons/physiology
  • Mice
  • Neurogenesis/genetics
  • Neurogenesis/physiology
  • Prosencephalon/embryology
  • RNA, Long Noncoding/genetics
  • Transcription Factors
Strain Development

The Evf1TS targeting construct was generated using lambda phage-based recombineering in E. coli. Following electroporation of W4 embryonic stem cells derived from 129S6/SvEvTac, chimeras were bred to C57BL/6J. Evf1TS(floxed neo)/+ heterozygotes were verified by Southern and crossed to FVB/N-Tg(EIIa-cre)C5379Lmgd/J (RRID:IMSR_JAX:003314) for two generations to remove the selection cassette, followed by additional backcrossing to C57BL/6J to N2 and sib-mating to homozygosity.

Suggested Control Mice
Wild-type littermates are used as controls

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

Research Applications

  • Neurobiology

Strain Origin

Donor
Jhumku Kohtz, Ph.D., Northwestern University.
Primary Reference

Cajigas I, Chakraborty A, Swyter KR, Luo H, Bastidas M, Nigro M, Morris ER, Chen S, VanGompel MJW, Leib D, Kohtz SJ, Martina M, Koh S, Ay F, Kohtz JD. The Evf2 Ultraconserved Enhancer lncRNA Functionally and Spatially Organizes Megabase Distant Genes in the Developing Forebrain. Mol Cell. 2018 Sep 20;71(6):956-972.e9. doi: 10.1016/j.molcel.2018.07.024. Epub 2018 Aug 23. (Medline PMID: 30146317)

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N2 (C57BL/6J), F?
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 7 to 9 months
Bred to Homozygosity
Yes
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sponsored Research.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sponsored Research

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068496-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit		 Aliquot Approximate quantity2 : 20-40 embryos / aliquot
068496-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit		 Aliquot Approximate quantity3
068496-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.