Strain Name:
C57BL/6J-MtgxR8528Btlr/Mmmh
Stock Number:
068498-MU
Citation ID:
RRID:MMRRC_068498-MU
Other Names:
R8528 (G1)
Major Collection:

Strain Information

Dpy30
Name: dpy-30, histone methyltransferase complex regulatory subunit
Synonyms: 2810410M20Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66310
VEGA: 17
Homologene: 32582
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, RP17, D17Rp17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Arl5b
Name: ADP-ribosylation factor-like 5B
Synonyms: 4930587A11Rik, Arl8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75869
Homologene: 101695
Stk24
Name: serine/threonine kinase 24
Synonyms: 1810013H02Rik, STE20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Rc3h2
Name: ring finger and CCCH-type zinc finger domains 2
Synonyms: Rnf164, D930043C02Rik, 2900024N03Rik, 9430019J22Rik, Mnab
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 319817
Homologene: 28276
Exog
Name: exo/endonuclease G
Synonyms: ENGL-a, ENDOGL2, ENGL-B, Endogl1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208194
VEGA: 9
HGNC: HGNC:3347
Homologene: 3745
Sorbs1
Name: sorbin and SH3 domain containing 1
Synonyms: c-Cbl-associated protein, CAP, Sh3d5, 9530001P15Rik, 2310065E01Rik, Ponsin
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 20411
VEGA: 19
Homologene: 83252
Cyp1b1
Name: cytochrome P450, family 1, subfamily b, polypeptide 1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 13078
VEGA: 17
HGNC: HGNC:2597
Homologene: 68035
Sprn
Name: shadow of prion protein
Synonyms: shadoo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 212518
Homologene: 84643
Myo9a
Name: myosin IXa
Synonyms: 4732465J09Rik, C130068I12Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 270163
HGNC: HGNC:7608
Homologene: 21371
Gfpt1
Name: glutamine fructose-6-phosphate transaminase 1
Synonyms: GFAT, GFA, GFAT1, 2810423A18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14583
HGNC: HGNC:4241
Homologene: 68220
Lrrc8a
Name: leucine rich repeat containing 8A VRAC subunit A
Synonyms: Lrrc8, ebo, SWELL1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241296
Homologene: 18617
Stim1
Name: stromal interaction molecule 1
Synonyms: SIM
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20866
Homologene: 20681
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23965
Homologene: 22673
Firrm
Name: FIGNL1 interacting regulator of recombination and mitosis
Synonyms: BC055324, Flip
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381306
Homologene: 10058
Sertad4
Name: SERTA domain containing 4
Synonyms: C130018M11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 214791
Homologene: 10483
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: lrd, b2b598Clo, b2b1289Clo, b2b1727Clo, b2b1203Clo, b2b1279Clo, Dnahc11, avc4
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320558
Homologene: 8604
Ahnak
Name: AHNAK nucleoprotein
Synonyms: DY6, 2310047C17Rik, 1110004P15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Cfap65
Name: cilia and flagella associated protein 65
Synonyms: B230363K08Rik, Ccdc108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241116
Homologene: 28093
Slc4a10
Name: solute carrier family 4, sodium bicarbonate cotransporter-like, member 10
Synonyms: NCBE
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94229
Homologene: 23340
Frmpd1
Name: FERM and PDZ domain containing 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 666060
Homologene: 8939
Kcnv2
Name: potassium channel, subfamily V, member 2
Synonyms: KV11.1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240595
Homologene: 26423
Spag17
Name: sperm associated antigen 17
Synonyms: 4931427F14Rik, PF6
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 74362
Homologene: 52601
Gsdmc
Name: gasdermin C
Synonyms: Mlze, Gsdmc1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 83492
HGNC: HGNC:7151
Homologene: 69487
Ccng2
Name: cyclin G2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12452
HGNC: HGNC:1593
Homologene: 3208
Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: B830018M07Rik, Bai1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18459
Homologene: 56509
Slc28a2
Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 2
Synonyms: CNT2, 2010208B10Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 269346
Homologene: 20891
Gm15446
Name: predicted gene 15446
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100642166
Fbxl12
Name: F-box and leucine-rich repeat protein 12
Synonyms: 3110048D16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30843
Homologene: 32198
Lrrc8d
Name: leucine rich repeat containing 8D
Synonyms: 4930525N13Rik, Lrrc5, 2810473G09Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231549
Homologene: 10004
Mapk8ip2
Name: mitogen-activated protein kinase 8 interacting protein 2
Synonyms: JNK-interacting protein, Jip2, 3230402N03Rik, IB2
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 60597
HGNC: HGNC:6883
Homologene: 8201
Ero1b
Name: endoplasmic reticulum oxidoreductase 1 beta
Synonyms: 1700065B09Rik, 1300013B24Rik, Ero1lb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 67475
VEGA: 13
Homologene: 8740
Kcnq5
Name: potassium voltage-gated channel, subfamily Q, member 5
Synonyms: 9230107O05Rik, D1Mgi1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226922
HGNC: HGNC:6299
Homologene: 28270
Hao1
Name: hydroxyacid oxidase 1, liver
Synonyms: GOX, Hao-1, Gox1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 15112
HGNC: HGNC:4809
Homologene: 6578
Pcdhga2
Name: protocadherin gamma subfamily A, 2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93710
HGNC: HGNC:8700
Homologene: 69262
Fer1l5
Name: fer-1 like family member 5
Synonyms: 4930533C12Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 100534273
Homologene: 85232
Dchs2
Name: dachsous cadherin related 2
Synonyms: LOC229459
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100534287
Eppk1
Name: epiplakin 1
Synonyms: EPIPL1, EPPK
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223650
VEGA: 15
Homologene: 20006
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 21,479,424 bp
  • A to G, chromosome 1 at 36,417,774 bp
  • A to G, chromosome 1 at 63,303,386 bp
  • A to G, chromosome 1 at 74,905,937 bp
  • T to C, chromosome 1 at 163,986,083 bp
  • A to G, chromosome 1 at 192,851,083 bp
  • T to A, chromosome 2 at 15,073,327 bp
  • T to C, chromosome 2 at 30,255,545 bp
  • G to A, chromosome 2 at 37,382,799 bp
  • T to C, chromosome 2 at 62,296,796 bp
  • A to G, chromosome 2 at 122,455,742 bp
  • A to G, chromosome 2 at 134,522,993 bp
  • A to C, chromosome 2 at 150,409,042 bp
  • A to G, chromosome 2 at 178,374,533 bp
  • T to C, chromosome 3 at 83,354,611 bp
  • A to T, chromosome 3 at 100,124,185 bp
  • T to C, chromosome 4 at 45,285,034 bp
  • T to A, chromosome 5 at 88,502,219 bp
  • T to C, chromosome 5 at 93,269,305 bp
  • T to A, chromosome 5 at 105,812,486 bp
  • T to C, chromosome 5 at 109,943,030 bp
  • G to C, chromosome 6 at 87,066,788 bp
  • T to C, chromosome 7 at 102,431,082 bp
  • C to T, chromosome 7 at 140,153,510 bp
  • C to T, chromosome 8 at 48,342,633 bp
  • C to A, chromosome 9 at 20,638,864 bp
  • A to G, chromosome 9 at 59,860,140 bp
  • A to G, chromosome 9 at 119,462,620 bp
  • A to G, chromosome 12 at 118,008,803 bp
  • T to A, chromosome 13 at 12,599,868 bp
  • C to A, chromosome 14 at 121,292,035 bp
  • A to T, chromosome 15 at 63,777,340 bp
  • A to C, chromosome 15 at 74,575,851 bp
  • C to T, chromosome 15 at 76,110,119 bp
  • A to G, chromosome 15 at 89,455,219 bp
  • T to C, chromosome 17 at 12,232,934 bp
  • T to C, chromosome 17 at 74,299,762 bp
  • T to A, chromosome 17 at 79,710,564 bp
  • G to T, chromosome 18 at 37,669,168 bp
  • C to T, chromosome 18 at 39,775,386 bp
  • A to T, chromosome 19 at 9,007,728 bp
  • T to A, chromosome 19 at 27,322,987 bp
  • G to C, chromosome 19 at 40,376,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068498-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.