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Strain Name:
C57BL/6J-MtgxR8528Btlr/Mmmh
Stock Number:
068498-MU
Citation ID:
RRID:MMRRC_068498-MU
Other Names:
R8528 (G1)
Major Collection:

Strain Information

Dpy30
Name: dpy-30, histone methyltransferase complex regulatory subunit
Synonyms: 2810410M20Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 66310
VEGA: 17
Homologene: 32582
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Map3k4
Name: mitogen-activated protein kinase kinase kinase 4
Synonyms: MTK1, MAPKKK4, D17Rp17, RP17, D17Rp17e, Mekk4, T-associated sex reversal, Tas
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26407
VEGA: 17
HGNC: HGNC:6856
Homologene: 31346
Arl5b
Name: ADP-ribosylation factor-like 5B
Synonyms: 4930587A11Rik, Arl8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 75869
Homologene: 101695
Stk24
Name: serine/threonine kinase 24
Synonyms: 1810013H02Rik, STE20
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 223255
VEGA: 14
Homologene: 20793
Rc3h2
Name: ring finger and CCCH-type zinc finger domains 2
Synonyms: Rnf164, D930043C02Rik, 2900024N03Rik, 9430019J22Rik, Mnab
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 319817
Homologene: 28276
Exog
Name: exo/endonuclease G
Synonyms: ENGL-a, ENDOGL2, ENGL-B, Endogl1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 208194
VEGA: 9
HGNC: HGNC:3347
Homologene: 3745
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 21,479,424 bp
  • A to G, chromosome 1 at 36,417,774 bp
  • A to G, chromosome 1 at 63,303,386 bp
  • A to G, chromosome 1 at 74,905,937 bp
  • T to C, chromosome 1 at 163,986,083 bp
  • A to G, chromosome 1 at 192,851,083 bp
  • T to A, chromosome 2 at 15,073,327 bp
  • T to C, chromosome 2 at 30,255,545 bp
  • G to A, chromosome 2 at 37,382,799 bp
  • T to C, chromosome 2 at 62,296,796 bp
  • A to G, chromosome 2 at 122,455,742 bp
  • A to G, chromosome 2 at 134,522,993 bp
  • A to C, chromosome 2 at 150,409,042 bp
  • A to G, chromosome 2 at 178,374,533 bp
  • T to C, chromosome 3 at 83,354,611 bp
  • A to T, chromosome 3 at 100,124,185 bp
  • T to C, chromosome 4 at 45,285,034 bp
  • T to A, chromosome 5 at 88,502,219 bp
  • T to C, chromosome 5 at 93,269,305 bp
  • T to A, chromosome 5 at 105,812,486 bp
  • T to C, chromosome 5 at 109,943,030 bp
  • G to C, chromosome 6 at 87,066,788 bp
  • T to C, chromosome 7 at 102,431,082 bp
  • C to T, chromosome 7 at 140,153,510 bp
  • C to T, chromosome 8 at 48,342,633 bp
  • C to A, chromosome 9 at 20,638,864 bp
  • A to G, chromosome 9 at 59,860,140 bp
  • A to G, chromosome 9 at 119,462,620 bp
  • A to G, chromosome 12 at 118,008,803 bp
  • T to A, chromosome 13 at 12,599,868 bp
  • C to A, chromosome 14 at 121,292,035 bp
  • A to T, chromosome 15 at 63,777,340 bp
  • A to C, chromosome 15 at 74,575,851 bp
  • C to T, chromosome 15 at 76,110,119 bp
  • A to G, chromosome 15 at 89,455,219 bp
  • T to C, chromosome 17 at 12,232,934 bp
  • T to C, chromosome 17 at 74,299,762 bp
  • T to A, chromosome 17 at 79,710,564 bp
  • G to T, chromosome 18 at 37,669,168 bp
  • C to T, chromosome 18 at 39,775,386 bp
  • A to T, chromosome 19 at 9,007,728 bp
  • T to A, chromosome 19 at 27,322,987 bp
  • G to C, chromosome 19 at 40,376,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8528 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068498-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.