Strain Name:
C57BL/6J-MtgxR8554Btlr/Mmmh
Stock Number:
068517-MU
Citation ID:
RRID:MMRRC_068517-MU
Other Names:
R8554 (G1)
Major Collection:

Strain Information

Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Rpsa
Name: ribosomal protein SA
Synonyms: P40-3, Lamr, Lamrl1, P40-8, 67lr, Lamr1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16785
VEGA: 9
HGNC: HGNC:6502
Homologene: 68249
Pitpnb
Name: phosphatidylinositol transfer protein, beta
Synonyms: Pitp beta
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 56305
HGNC: HGNC:9002
Homologene: 4533
Senp7
Name: SUMO1/sentrin specific peptidase 7
Synonyms: 6030449K19Rik, 2900036C23Rik, 2410152H17Rik, 2810413I22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66315
Homologene: 10778
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233532
Homologene: 41142
Ep300
Name: E1A binding protein p300
Synonyms: p300, KAT3B
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Ccdc60
Name: coiled-coil domain containing 60
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269693
Homologene: 18624
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Krt33a
Name: keratin 33A
Synonyms: 2310015J09Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 71888
Homologene: 74433
Apob
Name: apolipoprotein B
Synonyms: apob-100, apob-48
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Dsg4
Name: desmoglein 4
Synonyms: lah, CDHF13
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16769
Homologene: 65341
Fam193a
Name: family with sequence homology 193, member A
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231128
Homologene: 2746
Apc
Name: APC, WNT signaling pathway regulator
Synonyms: Min, CC1, adenomatosis polyposis coli
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 11789
HGNC: HGNC:583
Homologene: 30950
Usp42
Name: ubiquitin specific peptidase 42
Synonyms: 3110031A07Rik, 2410140K03Rik, D5Ertd591e, A630018G05Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 76800
Homologene: 35425
Rab35
Name: RAB35, member RAS oncogene family
Synonyms: RAB1C, H-ray, 9530019H02Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77407
HGNC: HGNC:9774
Homologene: 21361
Golga2
Name: golgin A2
Synonyms: GM130
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99412
HGNC: HGNC:4425
Homologene: 3300
Marchf6
Name: membrane associated ring-CH-type finger 6
Synonyms: F830029L24Rik, March6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 223455
VEGA: 15
Homologene: 4301
Rnf214
Name: ring finger protein 214
Synonyms: D130054N24Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235315
Homologene: 109357
Siglecg
Name: sialic acid binding Ig-like lectin G
Synonyms: mSiglec-G, A630096C01Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 243958
Homologene: 13228
Tmc2
Name: transmembrane channel-like gene family 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 192140
Homologene: 25877
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Rasef
Name: RAS and EF hand domain containing
Synonyms: RAB45
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242505
Homologene: 28424
Adtrp
Name: androgen dependent TFPI regulating protein
Synonyms: 9530008L14Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 109254
Homologene: 13107
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Tnn
Name: tenascin N
Synonyms: tenascin-W, Tnw
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329278
Homologene: 18634
Camk2d
Name: calcium/calmodulin-dependent protein kinase II, delta
Synonyms: CaMK II, 2810011D23Rik, 8030469K03Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 108058
HGNC: HGNC:1462
Homologene: 55561
Vmn2r1
Name: vomeronasal 2, receptor 1
Synonyms: EG56544, V2r83
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56544
Homologene: 84832
Eln
Name: elastin
Synonyms: tropoelastin, E030024M20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13717
HGNC: HGNC:3327
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 239420
Homologene: 65982
Rev3l
Name: REV3 like, DNA directed polymerase zeta catalytic subunit
Synonyms: Sez4, Rev
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19714
HGNC: HGNC:9968
Homologene: 48147
Or2h15
Name: olfactory receptor family 2 subfamily H member 15
Synonyms: GA_x6K02T2PSCP-2579687-2578746, MOR256-49, Olfr132
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 257889
Homologene: 46430
Rgl3
Name: ral guanine nucleotide dissociation stimulator-like 3
Synonyms: 1300003D20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71746
VEGA: 9
Homologene: 11363
Myom1
Name: myomesin 1
Synonyms: skelemin, D430047A17Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 17929
VEGA: 17
HGNC: HGNC:7613
Homologene: 31196
Tnfrsf8
Name: tumor necrosis factor receptor superfamily, member 8
Synonyms: CD30
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 21941
Homologene: 949
Crat
Name: carnitine acetyltransferase
Synonyms: CARAT
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12908
HGNC: HGNC:2342
Homologene: 598
Gm3486
Name: predicted gene 3486
Type: Gene
Species: Mouse
Chromosome: 14
Isg20
Name: interferon-stimulated protein
Synonyms: HEM45, DnaQl, 1600023I01Rik, 2010107M23Rik, 20kDa
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 57444
HGNC: HGNC:6130
Homologene: 31081
Vmn2r16
Name: vomeronasal 2, receptor 16
Synonyms: EG384220
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 384220
Homologene: 104825
Vmn2r65
Name: vomeronasal 2, receptor 65
Synonyms: ENSMUSG00000070600
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 100009609
Fcgr1
Name: Fc receptor, IgG, high affinity I
Synonyms: CD64, FcgammaRI
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14129
Homologene: 475
Pou2f2
Name: POU domain, class 2, transcription factor 2
Synonyms: Oct2b, Oct2a, Otf-2, Oct-2, Otf2, Oct2d, Oct2c
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 18987
HGNC: HGNC:9213
Homologene: 55674
Kics2
Name: KICSTOR subunit 2
Synonyms: BC048403
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 270802
Homologene: 17580
Or4c122
Name: olfactory receptor family 4 subfamily C member 122
Synonyms: GA_x6K02T2Q125-50696209-50695274, MOR233-1, Olfr1228
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258973
Homologene: 74217
Pate11
Name: prostate and testis expressed 11
Synonyms: Gm9513
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 671003
Homologene: 86450
Vmn1r29
Name: vomeronasal 1 receptor 29
Synonyms: V1rc2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113859
Homologene: 123821
Tent5a
Name: terminal nucleotidyltransferase 5A
Synonyms: D930050G01Rik, BAP014, Fam46a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 212943
Homologene: 23032
Rftn1
Name: raftlin lipid raft linker 1
Synonyms: 2310015N21Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 76438
Homologene: 18745
Cd47
Name: CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
Synonyms: Itgp, 9130415E20Rik, B430305P08Rik, integrin-associated protein, IAP
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 16423
HGNC: HGNC:1682
Homologene: 1346
Pdlim2
Name: PDZ and LIM domain 2
Synonyms: mystique, SLIM, 4732462F18Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 213019
Homologene: 11006
Vmn1r123
Name: vomeronasal 1 receptor 123
Synonyms: LOC384695, Gm1446
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 384695
Homologene: 104166
Or2aa1
Name: olfactory receptor family 2 subfamily AA member 1
Synonyms: GA_x6K02T000SA-78-1037, MOR256-30, Olfr223
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 258421
Homologene: 73917
Kdf1
Name: keratinocyte differentiation factor 1
Synonyms: 1810019J16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 69073
Homologene: 17563
Tlcd3a
Name: TLC domain containing 3A
Synonyms: 5430402E13Rik, 5430420K21Rik, Wdt3, 2310047D13Rik, 4932415L08Rik, Fam57a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 116972
Homologene: 11720
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 160,110,416 bp
  • A to G, chromosome 2 at 30,410,023 bp
  • G to A, chromosome 2 at 32,293,345 bp
  • T to A, chromosome 2 at 41,344,483 bp
  • T to C, chromosome 2 at 76,738,032 bp
  • T to C, chromosome 2 at 89,249,251 bp
  • A to G, chromosome 2 at 130,264,164 bp
  • T to C, chromosome 3 at 64,089,913 bp
  • A to T, chromosome 3 at 96,292,472 bp
  • A to T, chromosome 3 at 126,770,799 bp
  • A to T, chromosome 4 at 73,727,607 bp
  • A to G, chromosome 4 at 133,528,877 bp
  • A to C, chromosome 4 at 145,296,941 bp
  • T to A, chromosome 5 at 34,475,771 bp
  • A to G, chromosome 5 at 109,364,131 bp
  • T to C, chromosome 5 at 111,346,506 bp
  • A to C, chromosome 5 at 115,645,631 bp
  • A to T, chromosome 5 at 116,190,112 bp
  • A to C, chromosome 5 at 134,710,110 bp
  • T to A, chromosome 5 at 143,720,382 bp
  • T to A, chromosome 6 at 58,308,206 bp
  • T to A, chromosome 7 at 21,163,046 bp
  • C to A, chromosome 7 at 25,115,556 bp
  • C to T, chromosome 7 at 43,408,896 bp
  • T to C, chromosome 7 at 78,916,677 bp
  • T to C, chromosome 7 at 84,946,752 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,923 bp
  • A to G, chromosome 9 at 21,988,863 bp
  • A to G, chromosome 9 at 36,476,492 bp
  • C to A, chromosome 9 at 45,867,499 bp
  • T to C, chromosome 9 at 85,326,731 bp
  • T to C, chromosome 9 at 120,129,251 bp
  • T to C, chromosome 10 at 39,806,842 bp
  • T to A, chromosome 10 at 121,740,055 bp
  • A to T, chromosome 11 at 59,589,486 bp
  • A to G, chromosome 11 at 76,205,418 bp
  • T to A, chromosome 11 at 100,012,383 bp
  • A to C, chromosome 12 at 7,987,830 bp
  • G to A, chromosome 13 at 41,816,160 bp
  • T to A, chromosome 13 at 63,296,897 bp
  • T to A, chromosome 14 at 41,487,162 bp
  • T to A, chromosome 14 at 70,171,249 bp
  • G to A, chromosome 15 at 31,482,830 bp
  • T to C, chromosome 15 at 47,644,142 bp
  • A to G, chromosome 15 at 81,639,027 bp
  • A to G, chromosome 16 at 49,867,941 bp
  • T to C, chromosome 16 at 56,158,610 bp
  • T to A, chromosome 17 at 38,130,598 bp
  • G to T, chromosome 17 at 50,047,380 bp
  • A to G, chromosome 17 at 71,036,453 bp
  • G to T, chromosome 17 at 80,398,413 bp
  • T to A, chromosome 18 at 20,453,043 bp
  • T to C, chromosome 18 at 34,312,946 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8554 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068517-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.