Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8677Btlr/Mmmh
Stock Number:
068532-MU
Citation ID:
RRID:MMRRC_068532-MU
Other Names:
R8677 (G1)
Major Collection:

Strain Information

Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Nlgn3
Name: neuroligin 3
Synonyms: HNL3, A230085M13Rik, NL3, NLG3
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 245537
Homologene: 23133
Cd36
Name: CD36 molecule
Synonyms: FAT, fatty acid translocase, Scarb3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 12491
HGNC: HGNC:1663
Homologene: 73871
Hcn2
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 2
Synonyms: HAC1, trls
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15166
HGNC: HGNC:4846
Homologene: 31022
Ctnnal1
Name: catenin alpha like 1
Synonyms: ACRP, Catnal1, catenin (cadherin associated protein), alpha-like 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 54366
HGNC: HGNC:2512
Homologene: 2815
Comp
Name: cartilage oligomeric matrix protein
Synonyms: thrombospondin-5, TSP5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12845
HGNC: HGNC:2227
Homologene: 74
Mthfd1l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 270685
Homologene: 56706
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 94,041,227 bp
  • A to G, chromosome 1 at 138,083,597 bp
  • T to A, chromosome 2 at 3,220,315 bp
  • C to T, chromosome 2 at 26,469,924 bp
  • A to T, chromosome 2 at 67,516,634 bp
  • G to A, chromosome 2 at 158,751,178 bp
  • T to A, chromosome 2 at 164,488,602 bp
  • C to T, chromosome 2 at 180,172,039 bp
  • A to T, chromosome 3 at 27,457,027 bp
  • A to G, chromosome 3 at 53,477,701 bp
  • A to G, chromosome 3 at 55,502,419 bp
  • A to C, chromosome 3 at 83,928,702 bp
  • A to T, chromosome 3 at 92,572,679 bp
  • A to T, chromosome 3 at 138,104,676 bp
  • A to G, chromosome 4 at 56,813,272 bp
  • C to T, chromosome 4 at 147,863,046 bp
  • C to T, chromosome 5 at 17,820,495 bp
  • T to C, chromosome 5 at 52,184,544 bp
  • T to C, chromosome 5 at 68,110,414 bp
  • T to A, chromosome 5 at 111,419,019 bp
  • A to G, chromosome 6 at 24,796,985 bp
  • A to T, chromosome 6 at 42,290,585 bp
  • A to G, chromosome 6 at 55,872,594 bp
  • C to T, chromosome 7 at 6,072,645 bp
  • A to G, chromosome 7 at 24,180,645 bp
  • G to T, chromosome 7 at 86,165,202 bp
  • T to C, chromosome 7 at 102,000,941 bp
  • A to G, chromosome 7 at 105,157,568 bp
  • T to A, chromosome 7 at 142,356,012 bp
  • A to T, chromosome 8 at 70,380,260 bp
  • G to T, chromosome 8 at 94,779,815 bp
  • A to C, chromosome 8 at 105,700,944 bp
  • G to A, chromosome 9 at 36,910,591 bp
  • T to A, chromosome 9 at 48,489,973 bp
  • T to C, chromosome 9 at 50,658,707 bp
  • T to C, chromosome 9 at 73,932,961 bp
  • T to A, chromosome 10 at 4,048,250 bp
  • T to C, chromosome 10 at 17,915,676 bp
  • A to T, chromosome 10 at 39,923,809 bp
  • A to G, chromosome 10 at 79,724,785 bp
  • G to T, chromosome 10 at 82,620,216 bp
  • T to C, chromosome 11 at 23,595,471 bp
  • A to T, chromosome 11 at 69,735,555 bp
  • A to T, chromosome 11 at 74,195,589 bp
  • A to G, chromosome 11 at 77,455,193 bp
  • C to T, chromosome 11 at 78,284,156 bp
  • A to T, chromosome 11 at 100,519,743 bp
  • T to C, chromosome 11 at 102,433,567 bp
  • G to A, chromosome 11 at 120,647,989 bp
  • T to A, chromosome 14 at 23,386,350 bp
  • T to C, chromosome 14 at 57,923,963 bp
  • T to A, chromosome 15 at 81,814,968 bp
  • T to C, chromosome 15 at 98,642,050 bp
  • T to C, chromosome 16 at 64,926,051 bp
  • T to C, chromosome 17 at 32,396,854 bp
  • A to G, chromosome 17 at 65,926,407 bp
  • A to T, chromosome 17 at 66,024,214 bp
  • G to T, chromosome 17 at 75,348,758 bp
  • A to T, chromosome 17 at 75,512,060 bp
  • G to T, chromosome 18 at 38,282,138 bp
  • A to G, chromosome 18 at 53,738,561 bp
  • C to T, chromosome 19 at 5,286,229 bp
  • A to G, chromosome 19 at 8,386,671 bp
  • T to C, chromosome 19 at 47,651,801 bp
  • T to C, chromosome X at 101,308,784 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8677 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068532-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.