Strain Name:
C57BL/6J-MtgxR8686Btlr/Mmmh
Stock Number:
068541-MU
Citation ID:
RRID:MMRRC_068541-MU
Other Names:
R8686 (G1)
Major Collection:

Strain Information

Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Dgka
Name: diacylglycerol kinase, alpha
Synonyms: Dagk1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13139
VEGA: 10
HGNC: HGNC:2849
Homologene: 1028
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Nol10
Name: nucleolar protein 10
Synonyms: LOC217431
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217431
VEGA: 12
Homologene: 5998
Cenpf
Name: centromere protein F
Synonyms: 6530404A22Rik, Lek1, mitosin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Rraga
Name: Ras-related GTP binding A
Synonyms: FIP-1, 1300010C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68441
Homologene: 68522
Tfap2c
Name: transcription factor AP-2, gamma
Synonyms: Ap-2.2, Stra2, AP2gamma, Tcfap2c
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 21420
Homologene: 2423
Polr2b
Name: polymerase (RNA) II (DNA directed) polypeptide B
Synonyms: RPB2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231329
HGNC: HGNC:9188
Homologene: 722
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: 1810042B05Rik, Coh1, C330002D13Rik, 2310042E16Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Pfkl
Name: phosphofructokinase, liver, B-type
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18641
HGNC: HGNC:8876
Homologene: 55668
Lrch3
Name: leucine-rich repeats and calponin homology (CH) domain containing 3
Synonyms: LOC385628, 2210409B11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 70144
Homologene: 13111
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: 9330197E15Rik, LOC380767, A630028O16Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Cachd1
Name: cache domain containing 1
Synonyms: 1190007F10Rik, Vwcd1, B430218L07Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320508
Homologene: 10854
Kalrn
Name: kalirin, RhoGEF kinase
Synonyms: LOC224126, Hapip, 2210407G14Rik, E530005C20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 545156
HGNC: HGNC:4814
Homologene: 57160
Siglecf
Name: sialic acid binding Ig-like lectin F
Synonyms: mSiglec-F, Siglec5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233186
Homologene: 50482
Irf4
Name: interferon regulatory factor 4
Synonyms: Spip, IRF-4
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16364
HGNC: HGNC:6119
Homologene: 1842
Dnajc13
Name: DnaJ heat shock protein family (Hsp40) member C13
Synonyms: Rme8, LOC382100, D030002L11Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235567
Homologene: 13574
Cd22
Name: CD22 antigen
Synonyms: Lyb-8, Lyb8
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12483
HGNC: HGNC:1643
Homologene: 31052
Fgd2
Name: FYVE, RhoGEF and PH domain containing 2
Synonyms: Tcd-2, tcs-2, tcs2, Tcd2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 26382
HGNC: HGNC:3664
Homologene: 8438
Pla2g4e
Name: phospholipase A2, group IVE
Synonyms: 2310026J01Rik, Pla2epsilon
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329502
Homologene: 65339
Map4k1
Name: mitogen-activated protein kinase kinase kinase kinase 1
Synonyms: Hpk1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 26411
HGNC: HGNC:6863
Homologene: 5199
Nos3
Name: nitric oxide synthase 3, endothelial cell
Synonyms: eNOS, ecNOS, Nos-3, 2310065A03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18127
HGNC: HGNC:7876
Homologene: 504
Adamts6
Name: ADAM metallopeptidase with thrombospondin type 1 motif 6
Synonyms: ADAM-TS6, A930019D11Rik, b2b1879.1Clo, b2b2228Clo, b2b2187.1Clo, b2b2182Clo, b2b2029Clo
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 108154
VEGA: 13
HGNC: HGNC:222
Homologene: 82573
Cmya5
Name: cardiomyopathy associated 5
Synonyms: Myospryn, 2310076E16Rik, 2310076E21Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76469
VEGA: 13
Homologene: 137367
Pik3r4
Name: phosphoinositide-3-kinase regulatory subunit 4
Synonyms: Vps15, p150
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 75669
HGNC: HGNC:8982
Homologene: 24678
Unc80
Name: unc-80, NALCN activator
Synonyms: C230061B10Rik, C030018G13Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 329178
Homologene: 122243
Efr3b
Name: EFR3 homolog B
Synonyms: C030014M07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 668212
VEGA: 12
Homologene: 66195
Prss43
Name: serine protease 43
Synonyms: LOC272643, Tessp3
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 272643
Homologene: 138484
Col5a2
Name: collagen, type V, alpha 2
Synonyms: 1110014L14Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12832
HGNC: HGNC:2210
Homologene: 20119
Xylt1
Name: xylosyltransferase 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233781
Homologene: 32534
Phkg1
Name: phosphorylase kinase gamma 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18682
HGNC: HGNC:8930
Homologene: 68508
Dym
Name: dymeclin
Synonyms: 1810041M12Rik, C030019K18Rik, 4933427L07Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 69190
VEGA: 18
Homologene: 69237
Adam25
Name: ADAM metallopeptidase domain 25
Synonyms: testase 2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23793
HGNC: HGNC:199
Homologene: 128364
Impdh1
Name: inosine monophosphate dehydrogenase 1
Synonyms: B930086D20Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 23917
HGNC: HGNC:6052
Homologene: 68096
Pde1a
Name: phosphodiesterase 1A, calmodulin-dependent
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18573
HGNC: HGNC:8774
Homologene: 21043
Zfp521
Name: zinc finger protein 521
Synonyms: B930086A16Rik, Evi3
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225207
VEGA: 18
Homologene: 9151
Fam187b
Name: family with sequence similarity 187, member B
Synonyms: 1700020B09Rik, Tmem162
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76415
Homologene: 138431
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: 5830419M17Rik, gp115, EMILIN-1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100952
Homologene: 5117
Snx14
Name: sorting nexin 14
Synonyms: YR-14, C330035N22Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244962
Homologene: 17823
Mcur1
Name: mitochondrial calcium uniporter regulator 1
Synonyms: 6230416A05Rik, Ccdc90a
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76137
VEGA: 13
Homologene: 134447
Wiz
Name: widely-interspaced zinc finger motifs
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22404
Homologene: 7997
Inhca
Name: inhibitor of carbonic anhydrase
Synonyms: mICA, 1300017J02Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71775
Homologene: 826
Gpr137b
Name: G protein-coupled receptor 137B
Synonyms: C80741, 2310041G17Rik, Tm7sf1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 83924
VEGA: 13
Homologene: 2454
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Fbh1
Name: F-box DNA helicase 1
Synonyms: Fbx18, Fbxo18
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 50755
Homologene: 9272
Tfap2d
Name: transcription factor AP-2, delta
Synonyms: Tcfap2d
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226896
Homologene: 17700
Zfp664
Name: zinc finger protein 664
Synonyms: D930038J03Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 269704
Homologene: 65046
Or14j7
Name: olfactory receptor family 14 subfamily J member 7
Synonyms: GA_x6K02T2PSCP-2374126-2375048, MOR218-13, Olfr128
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 383243
Homologene: 134080
Lrif1
Name: ligand dependent nuclear receptor interacting factor 1
Synonyms: 2010012G17Rik, 4933421E11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 321000
Homologene: 10160
Alpl
Name: alkaline phosphatase, liver/bone/kidney
Synonyms: Akp-2, TNSALP, Akp2, TNAP, ALP
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11647
HGNC: HGNC:438
Homologene: 37314
Parp12
Name: poly (ADP-ribose) polymerase family, member 12
Synonyms: Zc3hdc1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243771
Homologene: 11236
Rap1b
Name: RAS related protein 1b
Synonyms: 2810443E11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 215449
VEGA: 10
Homologene: 68719
Rrp8
Name: ribosomal RNA processing 8
Synonyms: 2900001K19Rik, 1500003O22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101867
Homologene: 5621
Odad1
Name: outer dynein arm docking complex subunit 1
Synonyms: Ccdc114
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 211535
Homologene: 128610
Tmt1b
Name: thiol methyltransferase 1B
Synonyms: 0610006F02Rik, Mettl7b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 71664
VEGA: 10
Homologene: 12400
Speer4e1
Name: spermatogenesis associated glutamate (E)-rich protein 4E1
Synonyms: SPEER-4E, Speer4e
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 624245
Homologene: 69402
Spopfm2
Name: speckle-type BTB/POZ protein family member 2
Synonyms: Gm10696
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 100043188
Homologene: 128308
Or2d4
Name: olfactory receptor family 2 subfamily D member 4
Synonyms: GA_x6K02T2PBJ9-9325348-9324416, MOR260-3, Olfr710
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 258594
Homologene: 27218
G6pc1
Name: glucose-6-phosphatase catalytic subunit 1
Synonyms: G6Pase, G6pt, Glc-6-Pase, Glc-6-Pase-alpha, G6pc
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14377
HGNC: HGNC:4056
Homologene: 20079
Teddm3
Name: transmembrane epididymal family member 3
Synonyms: 2310042E22Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 66561
Homologene: 83314
Vmn1r116
Name: vomeronasal 1 receptor 116
Synonyms: Gm8547
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 667268
Homologene: 104166
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 19,108,284 bp
  • C to T, chromosome 1 at 45,421,987 bp
  • A to G, chromosome 1 at 66,612,268 bp
  • A to G, chromosome 1 at 118,836,687 bp
  • A to T, chromosome 1 at 189,659,604 bp
  • C to T, chromosome 2 at 11,755,658 bp
  • C to T, chromosome 2 at 79,927,742 bp
  • A to G, chromosome 2 at 120,244,691 bp
  • A to G, chromosome 2 at 172,552,006 bp
  • T to C, chromosome 3 at 94,176,120 bp
  • C to T, chromosome 3 at 106,732,781 bp
  • C to T, chromosome 4 at 51,229,651 bp
  • A to G, chromosome 4 at 86,576,811 bp
  • G to A, chromosome 4 at 100,988,128 bp
  • A to T, chromosome 4 at 137,743,801 bp
  • T to C, chromosome 5 at 14,934,115 bp
  • A to G, chromosome 5 at 24,368,843 bp
  • A to T, chromosome 5 at 30,917,696 bp
  • T to C, chromosome 5 at 77,335,663 bp
  • T to A, chromosome 5 at 124,886,069 bp
  • A to T, chromosome 5 at 129,866,215 bp
  • C to A, chromosome 6 at 29,216,215 bp
  • A to T, chromosome 6 at 39,117,922 bp
  • T to C, chromosome 7 at 20,872,691 bp
  • A to G, chromosome 7 at 28,994,073 bp
  • G to A, chromosome 7 at 30,870,069 bp
  • T to C, chromosome 7 at 30,977,234 bp
  • T to C, chromosome 7 at 43,355,606 bp
  • A to G, chromosome 7 at 45,947,692 bp
  • A to T, chromosome 7 at 105,733,574 bp
  • A to C, chromosome 7 at 106,944,698 bp
  • G to C, chromosome 7 at 117,381,359 bp
  • A to G, chromosome 8 at 40,755,484 bp
  • G to T, chromosome 8 at 71,334,322 bp
  • T to A, chromosome 9 at 88,415,693 bp
  • C to T, chromosome 9 at 103,259,428 bp
  • T to C, chromosome 9 at 104,170,805 bp
  • A to G, chromosome 9 at 105,658,529 bp
  • C to T, chromosome 9 at 110,829,426 bp
  • A to T, chromosome 10 at 77,997,522 bp
  • C to A, chromosome 10 at 81,311,993 bp
  • A to T, chromosome 10 at 117,822,841 bp
  • A to G, chromosome 10 at 128,733,093 bp
  • A to T, chromosome 10 at 128,960,607 bp
  • T to G, chromosome 11 at 101,374,707 bp
  • A to T, chromosome 12 at 4,000,886 bp
  • T to A, chromosome 12 at 17,369,771 bp
  • T to C, chromosome 12 at 79,287,453 bp
  • T to C, chromosome 12 at 115,980,904 bp
  • T to C, chromosome 13 at 13,359,406 bp
  • A to T, chromosome 13 at 30,761,450 bp
  • G to A, chromosome 13 at 43,541,717 bp
  • A to T, chromosome 13 at 93,095,380 bp
  • A to G, chromosome 13 at 104,313,699 bp
  • C to G, chromosome 15 at 35,925,389 bp
  • A to G, chromosome 16 at 21,152,935 bp
  • T to C, chromosome 16 at 32,981,853 bp
  • A to G, chromosome 16 at 34,360,935 bp
  • C to T, chromosome 17 at 29,379,023 bp
  • T to A, chromosome 17 at 32,367,847 bp
  • T to C, chromosome 17 at 37,924,277 bp
  • A to C, chromosome 18 at 13,845,644 bp
  • T to A, chromosome 18 at 75,286,683 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8686 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068541-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.