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Strain Name:
C57BL/6J-MtgxR8686Btlr/Mmmh
Stock Number:
068541-MU
Citation ID:
RRID:MMRRC_068541-MU
Other Names:
R8686 (G1)
Major Collection:

Strain Information

Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Dgka
Name: diacylglycerol kinase, alpha
Synonyms: Dagk1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 13139
VEGA: 10
HGNC: HGNC:2849
Homologene: 1028
Pip5k1c
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma
Synonyms: PIP5KIgamma
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 18717
HGNC: HGNC:8996
Homologene: 69032
Nol10
Name: nucleolar protein 10
Synonyms: LOC217431
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217431
VEGA: 12
Homologene: 5998
Cenpf
Name: centromere protein F
Synonyms: 6530404A22Rik, Lek1, mitosin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Rraga
Name: Ras-related GTP binding A
Synonyms: FIP-1, 1300010C19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 68441
Homologene: 68522
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 19,108,284 bp
  • C to T, chromosome 1 at 45,421,987 bp
  • A to G, chromosome 1 at 66,612,268 bp
  • A to G, chromosome 1 at 118,836,687 bp
  • A to T, chromosome 1 at 189,659,604 bp
  • C to T, chromosome 2 at 11,755,658 bp
  • C to T, chromosome 2 at 79,927,742 bp
  • A to G, chromosome 2 at 120,244,691 bp
  • A to G, chromosome 2 at 172,552,006 bp
  • T to C, chromosome 3 at 94,176,120 bp
  • C to T, chromosome 3 at 106,732,781 bp
  • C to T, chromosome 4 at 51,229,651 bp
  • A to G, chromosome 4 at 86,576,811 bp
  • G to A, chromosome 4 at 100,988,128 bp
  • A to T, chromosome 4 at 137,743,801 bp
  • T to C, chromosome 5 at 14,934,115 bp
  • A to G, chromosome 5 at 24,368,843 bp
  • A to T, chromosome 5 at 30,917,696 bp
  • T to C, chromosome 5 at 77,335,663 bp
  • T to A, chromosome 5 at 124,886,069 bp
  • A to T, chromosome 5 at 129,866,215 bp
  • C to A, chromosome 6 at 29,216,215 bp
  • A to T, chromosome 6 at 39,117,922 bp
  • T to C, chromosome 7 at 20,872,691 bp
  • A to G, chromosome 7 at 28,994,073 bp
  • G to A, chromosome 7 at 30,870,069 bp
  • T to C, chromosome 7 at 30,977,234 bp
  • T to C, chromosome 7 at 43,355,606 bp
  • A to G, chromosome 7 at 45,947,692 bp
  • A to T, chromosome 7 at 105,733,574 bp
  • A to C, chromosome 7 at 106,944,698 bp
  • G to C, chromosome 7 at 117,381,359 bp
  • A to G, chromosome 8 at 40,755,484 bp
  • G to T, chromosome 8 at 71,334,322 bp
  • T to A, chromosome 9 at 88,415,693 bp
  • C to T, chromosome 9 at 103,259,428 bp
  • T to C, chromosome 9 at 104,170,805 bp
  • A to G, chromosome 9 at 105,658,529 bp
  • C to T, chromosome 9 at 110,829,426 bp
  • A to T, chromosome 10 at 77,997,522 bp
  • C to A, chromosome 10 at 81,311,993 bp
  • A to T, chromosome 10 at 117,822,841 bp
  • A to G, chromosome 10 at 128,733,093 bp
  • A to T, chromosome 10 at 128,960,607 bp
  • T to G, chromosome 11 at 101,374,707 bp
  • A to T, chromosome 12 at 4,000,886 bp
  • T to A, chromosome 12 at 17,369,771 bp
  • T to C, chromosome 12 at 79,287,453 bp
  • T to C, chromosome 12 at 115,980,904 bp
  • T to C, chromosome 13 at 13,359,406 bp
  • A to T, chromosome 13 at 30,761,450 bp
  • G to A, chromosome 13 at 43,541,717 bp
  • A to T, chromosome 13 at 93,095,380 bp
  • A to G, chromosome 13 at 104,313,699 bp
  • C to G, chromosome 15 at 35,925,389 bp
  • A to G, chromosome 16 at 21,152,935 bp
  • T to C, chromosome 16 at 32,981,853 bp
  • A to G, chromosome 16 at 34,360,935 bp
  • C to T, chromosome 17 at 29,379,023 bp
  • T to A, chromosome 17 at 32,367,847 bp
  • T to C, chromosome 17 at 37,924,277 bp
  • A to C, chromosome 18 at 13,845,644 bp
  • T to A, chromosome 18 at 75,286,683 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8686 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068541-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.