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Strain Name:
C57BL/6J-MtgxR8696Btlr/Mmmh
Stock Number:
068550-MU
Citation ID:
RRID:MMRRC_068550-MU
Other Names:
R8696 (G1)
Major Collection:

Strain Information

Myo10
Name: myosin X
Synonyms: myosin-X, D15Ertd600e
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17909
HGNC: HGNC:7593
Homologene: 36328
Chrna6
Name: cholinergic receptor, nicotinic, alpha polypeptide 6
Synonyms: Acra6, alpha6 nAChR
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11440
Homologene: 20888
Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, Cspg2, DPEAAE
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Nlgn3
Name: neuroligin 3
Synonyms: HNL3, A230085M13Rik, NL3, NLG3
Type: Gene
Species: Mouse
Chromosome: X
NCBI: 245537
Homologene: 23133
Krt5
Name: keratin 5
Synonyms: 3300001P10Rik, Tfip8, K5, Krt2-5
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 110308
HGNC: HGNC:6442
Homologene: 55461
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Cdk11b
Name: cyclin dependent kinase 11B
Synonyms: CDK11-p110, PITSLRE proteins, CDK11-p46, CDK11-p58, Cdc2l2, Cdc2l1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12537
Homologene: 22416
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 86,791,440 bp
  • A to G, chromosome 1 at 170,912,079 bp
  • C to T, chromosome 1 at 189,657,997 bp
  • T to A, chromosome 2 at 26,477,992 bp
  • T to C, chromosome 2 at 32,400,065 bp
  • A to T, chromosome 2 at 79,281,781 bp
  • T to C, chromosome 2 at 106,001,970 bp
  • A to T, chromosome 2 at 120,701,114 bp
  • T to A, chromosome 2 at 129,125,651 bp
  • C to T, chromosome 3 at 10,193,987 bp
  • T to C, chromosome 3 at 75,613,237 bp
  • T to C, chromosome 3 at 79,591,382 bp
  • T to A, chromosome 3 at 82,363,361 bp
  • T to C, chromosome 3 at 151,542,707 bp
  • G to A, chromosome 4 at 33,231,181 bp
  • A to G, chromosome 4 at 43,836,193 bp
  • G to A, chromosome 4 at 115,565,028 bp
  • C to T, chromosome 4 at 118,917,438 bp
  • T to G, chromosome 4 at 119,377,308 bp
  • T to C, chromosome 4 at 132,363,794 bp
  • T to C, chromosome 4 at 155,648,322 bp
  • C to T, chromosome 5 at 9,512,311 bp
  • A to G, chromosome 5 at 64,926,751 bp
  • T to G, chromosome 5 at 92,289,786 bp
  • T to C, chromosome 5 at 101,659,647 bp
  • A to G, chromosome 5 at 120,127,067 bp
  • A to T, chromosome 5 at 135,734,258 bp
  • T to A, chromosome 5 at 143,224,999 bp
  • G to T, chromosome 6 at 23,969,345 bp
  • C to T, chromosome 6 at 34,392,132 bp
  • G to T, chromosome 6 at 38,636,495 bp
  • T to A, chromosome 6 at 57,647,149 bp
  • C to A, chromosome 6 at 88,103,377 bp
  • A to G, chromosome 6 at 89,870,339 bp
  • A to T, chromosome 6 at 132,600,359 bp
  • A to G, chromosome 6 at 149,513,787 bp
  • T to C, chromosome 7 at 7,205,519 bp
  • A to T, chromosome 7 at 17,160,012 bp
  • T to C, chromosome 8 at 3,603,614 bp
  • G to T, chromosome 8 at 27,407,167 bp
  • T to A, chromosome 8 at 45,795,649 bp
  • T to A, chromosome 8 at 128,988,763 bp
  • A to G, chromosome 9 at 32,248,503 bp
  • A to G, chromosome 9 at 38,143,137 bp
  • A to C, chromosome 9 at 107,619,769 bp
  • T to G, chromosome 9 at 119,943,911 bp
  • T to A, chromosome 10 at 3,260,265 bp
  • A to G, chromosome 10 at 8,733,695 bp
  • C to T, chromosome 10 at 80,644,681 bp
  • A to T, chromosome 10 at 99,758,964 bp
  • A to T, chromosome 10 at 129,790,693 bp
  • T to G, chromosome 11 at 30,809,896 bp
  • C to T, chromosome 11 at 95,121,770 bp
  • T to C, chromosome 11 at 101,209,479 bp
  • T to C, chromosome 11 at 101,733,132 bp
  • T to A, chromosome 12 at 17,279,661 bp
  • T to C, chromosome 12 at 57,737,706 bp
  • T to C, chromosome 12 at 71,063,316 bp
  • A to G, chromosome 13 at 14,357,158 bp
  • A to G, chromosome 13 at 54,737,951 bp
  • A to G, chromosome 13 at 61,537,707 bp
  • A to G, chromosome 13 at 74,372,480 bp
  • A to T, chromosome 13 at 89,691,098 bp
  • A to T, chromosome 14 at 18,216,661 bp
  • A to G, chromosome 14 at 50,413,963 bp
  • A to T, chromosome 14 at 63,380,700 bp
  • T to G, chromosome 14 at 73,166,865 bp
  • C to G, chromosome 15 at 25,799,486 bp
  • T to C, chromosome 15 at 64,815,386 bp
  • T to C, chromosome 15 at 80,381,974 bp
  • A to G, chromosome 15 at 101,116,983 bp
  • A to G, chromosome 15 at 101,710,307 bp
  • A to G, chromosome 17 at 13,122,965 bp
  • A to T, chromosome 17 at 20,503,157 bp
  • A to T, chromosome 17 at 58,294,299 bp
  • G to A, chromosome 18 at 6,788,635 bp
  • G to A, chromosome 19 at 30,549,288 bp
  • A to G, chromosome 19 at 34,937,352 bp
  • T to A, chromosome 19 at 43,874,223 bp
  • A to G, chromosome 19 at 46,368,118 bp
  • T to C, chromosome X at 101,308,784 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8696 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068550-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.