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Strain Name:
C57BL/6J-MtgxR8699Btlr/Mmmh
Stock Number:
068553-MU
Citation ID:
RRID:MMRRC_068553-MU
Other Names:
R8699 (G1)
Major Collection:

Strain Information

Rest
Name: RE1-silencing transcription factor
Synonyms: 2610008J04Rik, NRSF
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 19712
HGNC: HGNC:9966
Homologene: 4099
Htr4
Name: 5 hydroxytryptamine (serotonin) receptor 4
Synonyms: 5-HT4L, 5-HT4
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 15562
VEGA: 18
HGNC: HGNC:5299
Homologene: 20243
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Npy5r
Name: neuropeptide Y receptor Y5
Synonyms: Y5R
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18168
HGNC: HGNC:7958
Homologene: 21241
Ppp1r9a
Name: protein phosphatase 1, regulatory subunit 9A
Synonyms: 4930518N04Rik, neurabin-I, A230094E16Rik, 2810430P21Rik, Neurabin I, NRB
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 243725
Homologene: 14247
Bclaf1
Name: BCL2-associated transcription factor 1
Synonyms: 2700025J07Rik, 2810454G14Rik, 2610102K23Rik, 5730534O06Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 72567
Homologene: 8832
Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Glur-4, Glur4, spkw1, Gluralpha4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 39,976,750 bp
  • T to A, chromosome 1 at 52,071,937 bp
  • A to G, chromosome 1 at 75,212,038 bp
  • T to A, chromosome 1 at 82,455,734 bp
  • T to A, chromosome 1 at 150,418,021 bp
  • T to A, chromosome 1 at 173,341,116 bp
  • A to G, chromosome 1 at 188,911,377 bp
  • C to A, chromosome 2 at 13,383,959 bp
  • A to T, chromosome 2 at 41,282,195 bp
  • A to G, chromosome 2 at 52,147,234 bp
  • G to A, chromosome 2 at 52,212,551 bp
  • A to T, chromosome 2 at 85,647,986 bp
  • A to T, chromosome 2 at 86,085,174 bp
  • T to C, chromosome 2 at 93,956,982 bp
  • T to C, chromosome 2 at 127,121,176 bp
  • T to C, chromosome 2 at 128,641,453 bp
  • A to G, chromosome 2 at 180,101,356 bp
  • T to G, chromosome 3 at 122,266,323 bp
  • C to A, chromosome 3 at 130,005,004 bp
  • A to T, chromosome 4 at 11,528,678 bp
  • A to T, chromosome 4 at 24,507,363 bp
  • T to C, chromosome 4 at 62,532,278 bp
  • A to T, chromosome 4 at 76,041,392 bp
  • A to G, chromosome 4 at 120,921,996 bp
  • A to T, chromosome 4 at 143,897,192 bp
  • C to T, chromosome 4 at 152,279,101 bp
  • T to C, chromosome 5 at 31,076,261 bp
  • T to C, chromosome 5 at 35,701,891 bp
  • G to A, chromosome 5 at 77,281,542 bp
  • T to A, chromosome 5 at 110,080,526 bp
  • T to TCCA, chromosome 6 at 4,756,451 bp
  • A to G, chromosome 6 at 5,115,474 bp
  • G to T, chromosome 6 at 48,597,345 bp
  • T to A, chromosome 6 at 90,178,760 bp
  • G to T, chromosome 7 at 15,989,188 bp
  • C to T, chromosome 7 at 23,606,655 bp
  • G to T, chromosome 7 at 23,808,809 bp
  • A to T, chromosome 7 at 27,668,355 bp
  • A to G, chromosome 7 at 41,648,416 bp
  • G to A, chromosome 7 at 51,593,771 bp
  • A to T, chromosome 7 at 103,639,615 bp
  • G to T, chromosome 7 at 127,786,602 bp
  • G to T, chromosome 8 at 66,681,622 bp
  • T to C, chromosome 8 at 71,709,633 bp
  • A to T, chromosome 8 at 112,757,596 bp
  • C to A, chromosome 9 at 4,424,347 bp
  • T to G, chromosome 9 at 4,424,351 bp
  • A to C, chromosome 9 at 8,087,361 bp
  • G to T, chromosome 9 at 60,871,065 bp
  • A to T, chromosome 10 at 5,918,194 bp
  • T to C, chromosome 10 at 20,333,438 bp
  • A to G, chromosome 10 at 62,432,455 bp
  • G to T, chromosome 11 at 82,794,550 bp
  • A to T, chromosome 11 at 101,582,294 bp
  • A to G, chromosome 11 at 104,781,246 bp
  • A to G, chromosome 11 at 117,783,535 bp
  • T to C, chromosome 12 at 116,207,701 bp
  • A to T, chromosome 13 at 52,508,107 bp
  • A to G, chromosome 14 at 16,242,631 bp
  • G to A, chromosome 14 at 26,940,255 bp
  • G to A, chromosome 14 at 62,292,830 bp
  • A to G, chromosome 15 at 98,565,114 bp
  • A to T, chromosome 16 at 5,063,703 bp
  • A to G, chromosome 16 at 23,431,383 bp
  • A to G, chromosome 16 at 59,554,928 bp
  • T to C, chromosome 16 at 90,931,057 bp
  • C to T, chromosome 17 at 8,256,566 bp
  • A to G, chromosome 17 at 24,408,225 bp
  • C to T, chromosome 17 at 29,842,374 bp
  • T to A, chromosome 17 at 33,948,852 bp
  • A to G, chromosome 18 at 22,434,607 bp
  • A to T, chromosome 18 at 36,931,023 bp
  • A to T, chromosome 18 at 37,742,922 bp
  • G to A, chromosome 18 at 62,183,972 bp
  • G to A, chromosome 18 at 62,437,692 bp
  • T to A, chromosome 19 at 3,496,587 bp
  • A to G, chromosome 19 at 10,725,192 bp
  • A to G, chromosome 19 at 12,674,882 bp
  • A to T, chromosome 19 at 12,901,464 bp
  • G to C, chromosome 19 at 40,376,800 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8699 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068553-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.