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Strain Name:
C57BL/6J-MtgxR8711Btlr/Mmmh
Stock Number:
068565-MU
Citation ID:
RRID:MMRRC_068565-MU
Other Names:
R8711 (G1)
Major Collection:

Strain Information

Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: 1110067B02Rik, Sema6D-6, Sema6D-5, Sema6D-4, Sema6D-2, Sema6D-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 214968
Homologene: 16195
Ppp1r16b
Name: protein phosphatase 1, regulatory subunit 16B
Synonyms: ANKRD4, Wdt4, C130078N17Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228852
Homologene: 9194
Eif4g2
Name: eukaryotic translation initiation factor 4, gamma 2
Synonyms: Nat1, DAP-5, Natm1, E130105L11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 13690
HGNC: HGNC:3297
Homologene: 37477
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Sin3b
Name: transcriptional regulator, SIN3B (yeast)
Synonyms: 2810430C10Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20467
Homologene: 81810
Tcf12
Name: transcription factor 12
Synonyms: ME1, ALF1, HEB, HTF4, HTF-4, REB, bHLHb20, HEBAlt
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 21406
Homologene: 40774
Ell
Name: elongation factor RNA polymerase II
Synonyms: Men, Ell1, eleven-nineteen lysine-rich leukemia gene
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13716
Homologene: 4762
Laptm4b
Name: lysosomal-associated protein transmembrane 4B
Synonyms: C330023P13Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 114128
VEGA: 15
Homologene: 10182
Gng2
Name: guanine nucleotide binding protein (G protein), gamma 2
Synonyms: 1110003P13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 14702
HGNC: HGNC:4404
Homologene: 40715
Chd4
Name: chromodomain helicase DNA binding protein 4
Synonyms: D6Ertd380e, 9530019N15Rik, Mi-2beta
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107932
HGNC: HGNC:1919
Homologene: 68175
Fasn
Name: fatty acid synthase
Synonyms: FAS, A630082H08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14104
HGNC: HGNC:3594
Homologene: 55800
Srsf5
Name: serine and arginine-rich splicing factor 5
Synonyms: Sfrs5
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20384
Homologene: 133842
Smc5
Name: structural maintenance of chromosomes 5
Synonyms: Smc5l1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 226026
VEGA: 19
Homologene: 41009
Polr1b
Name: polymerase (RNA) I polypeptide B
Synonyms: RPA2, RPA116, 128kDa, D630020H17Rik, Rpo1-2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20017
Homologene: 7133
Slc43a2
Name: solute carrier family 43, member 2
Synonyms: 7630402D21Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 215113
Homologene: 17555
Atp5f1b
Name: ATP synthase F1 subunit beta
Synonyms: Atp5b
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11947
HGNC: HGNC:830
Homologene: 1273
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 108837
Homologene: 34661
Morc2a
Name: microrchidia 2A
Synonyms: 8430403M08Rik, Zcwcc1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74522
Homologene: 8966
Pkd1l1
Name: polycystic kidney disease 1 like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 171395
Homologene: 51376
Trpa1
Name: transient receptor potential cation channel, subfamily A, member 1
Synonyms: ANKTM1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 277328
HGNC: HGNC:497
Homologene: 7189
Slc25a47
Name: solute carrier family 25, member 47
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 104910
VEGA: 12
Homologene: 64441
Tnfrsf11b
Name: tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Synonyms: osteoclastogenesis inhibitory factor, OCIF, OPG, Opg, TR1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 18383
VEGA: 15
Homologene: 1912
Syne2
Name: spectrin repeat containing, nuclear envelope 2
Synonyms: nesprin-2, syne-2, D12Ertd777e, 6820443O06Rik, Nesp2g, Cpfl8, diminished cone electroretinogram, dice
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319565
Homologene: 56700
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237954
Homologene: 138824
Fat2
Name: FAT atypical cadherin 2
Synonyms: LOC245827, mKIAA0811, Fath2, EMI2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 245827
HGNC: HGNC:3596
Homologene: 1110
Slc9b2
Name: solute carrier family 9, subfamily B (NHA2, cation proton antiporter 2), member 2
Synonyms: C80638, NHA2, nha-oc, NHE10, Nhedc2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 97086
Homologene: 45381
Sh3rf1
Name: SH3 domain containing ring finger 1
Synonyms: Posh, 2200003J05Rik, Sh3md2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 59009
Homologene: 10988
Il11ra1
Name: interleukin 11 receptor subunit alpha 1
Synonyms: NR1, Il11ra, Il-11ra-alpha, Il-11ra
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 16157
HGNC: HGNC:5967
Homologene: 88705
Fhad1
Name: forkhead-associated phosphopeptide binding domain 1
Synonyms: 2900090M10Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 329977
Homologene: 77947
Aadat
Name: aminoadipate aminotransferase
Synonyms: mKat-2, Kat2, KATII, Kyat2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 23923
Homologene: 56540
Fsip2
Name: fibrous sheath-interacting protein 2
Synonyms: OTTMUSG00000013335
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241516
Homologene: 110349
Ermap
Name: erythroblast membrane-associated protein
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 27028
Homologene: 22782
Gm11639
Name: predicted gene 11639
Synonyms: Gm11639, Efcab15
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 105242472
Cyp4f40
Name: cytochrome P450, family 4, subfamily f, polypeptide 40
Synonyms: EG631304
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 631304
VEGA: 17
Homologene: 117991
Abcb11
Name: ATP-binding cassette, sub-family B member 11
Synonyms: PFIC2, ABC16, PGY4, Lith1, sister of P-glycoprotein, Bsep
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27413
HGNC: HGNC:42
Homologene: 74509
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 380698
Homologene: 70869
Zc3h12c
Name: zinc finger CCCH type containing 12C
Synonyms: C230027N18Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 244871
VEGA: 9
Homologene: 35462
Sycp2
Name: synaptonemal complex protein 2
Synonyms: 3830402K23Rik, 4930518F03Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320558
Homologene: 8604
Ltbp2
Name: latent transforming growth factor beta binding protein 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 16997
HGNC: HGNC:6715
Homologene: 369
Flad1
Name: flavin adenine dinucleotide synthetase 1
Synonyms: Pp591, A930017E24Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 319945
Homologene: 115598
Prkce
Name: protein kinase C, epsilon
Synonyms: PKC[e], Pkce, 5830406C15Rik, PKCepsilon, PCK epsilon
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18754
HGNC: HGNC:9401
Homologene: 48343
Cytip
Name: cytohesin 1 interacting protein
Synonyms: Cbp, A130053M09Rik, Pscdbp, Cybr
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227929
HGNC: HGNC:9506
Homologene: 3167
Tbc1d9b
Name: TBC1 domain family, member 9B
Synonyms: 2700008N14Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76795
Homologene: 28106
Cyp2j5
Name: cytochrome P450, family 2, subfamily j, polypeptide 5
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13109
HGNC: HGNC:2634
Homologene: 116530
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227058
Homologene: 41287
Obox3
Name: oocyte specific homeobox 3
Synonyms: Ohx
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 246791
Homologene: 44937
Rchy1
Name: ring finger and CHY zinc finger domain containing 1
Synonyms: PRO1996, 6720407C15Rik, Zfp363, Pirh2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 68098
Homologene: 22894
Ugt1a6a
Name: UDP glucuronosyltransferase 1 family, polypeptide A6A
Synonyms: UGT1.6, Ugt1a6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 94284
Homologene: 85959
Synj1
Name: synaptojanin 1
Synonyms: A930006D20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104015
Homologene: 48252
Or4a74
Name: olfactory receptor family 4 subfamily A member 74
Synonyms: GA_x6K02T2Q125-51051555-51050611, MOR231-6, Olfr1247
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258968
Homologene: 121536
Irf2bpl
Name: interferon regulatory factor 2 binding protein-like
Synonyms: 6430527G18Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 238330
VEGA: 12
Homologene: 11555
Gabrp
Name: gamma-aminobutyric acid type A receptor subunit pi
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216643
HGNC: HGNC:4089
Homologene: 22798
Slc43a3
Name: solute carrier family 43, member 3
Synonyms: SEEEG-1, Eeg1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 58207
Homologene: 40925
Hmgxb3
Name: HMG box domain containing 3
Synonyms: 2510002C16Rik, A630042L21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 106894
VEGA: 18
Homologene: 44229
Fpr-rs3
Name: formyl peptide receptor, related sequence 3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 14290
HGNC: HGNC:3827
Homologene: 130087
Smpdl3b
Name: sphingomyelin phosphodiesterase, acid-like 3B
Synonyms: 1110054A24Rik, Asml3b
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100340
Homologene: 8708
Vmn2r8
Name: vomeronasal 2, receptor 8
Synonyms: EG627479
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 627479
Homologene: 129606
Dohh
Name: deoxyhypusine hydroxylase/monooxygenase
Synonyms: 1110033C18Rik, Hlrc1
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 102115
Homologene: 6290
Ccdc85a
Name: coiled-coil domain containing 85A
Synonyms: E030025D05Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216613
Homologene: 65605
Or4p19
Name: olfactory receptor family 4 subfamily P member 19
Synonyms: GA_x6K02T2Q125-49900552-49899623, MOR225-1, Olfr1180
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258920
Homologene: 74240
Spred3
Name: sprouty-related EVH1 domain containing 3
Synonyms: Spred-3, D130060H24Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 101809
Homologene: 28061
Scpppq1
Name: secretory calcium-binding phosphoprotein proline-glutamine rich 1
Synonyms: Gm17660
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100271704
Homologene: 130040
Zfp952
Name: zinc finger protein 952
Synonyms: C920016K16Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240067
Homologene: 128790
Klk1b5
Name: kallikrein 1-related peptidase b5
Synonyms: mGK-5, Klk5
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16622
Homologene: 68141
Neurl1b
Name: neuralized E3 ubiquitin protein ligase 1B
Synonyms: EG240055, C230078M08Rik, Neur2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240055
Homologene: 35443
Col10a1
Name: collagen, type X, alpha 1
Synonyms: Col10a-1, Col10
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12813
HGNC: HGNC:2185
Homologene: 55466
Naif1
Name: nuclear apoptosis inducing factor 1
Synonyms: 2310007O20Rik, 4933440H19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71254
Homologene: 45669
Zbtb39
Name: zinc finger and BTB domain containing 39
Synonyms: 7030401O21Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 320080
VEGA: 10
Homologene: 8890
C1qbp
Name: complement component 1, q subcomponent binding protein
Synonyms: P32, HABP1, D11Wsu182e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12261
HGNC: HGNC:1243
Homologene: 31023
Gm16686
Name: predicted gene, 16686
Type: Gene
Species: Mouse
Chromosome: 4
Eif1ad19
Name: eukaryotic translation initiation factor 1A domain containing 19
Synonyms: Gm21319
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 100861908
Homologene: 103865
Trbv13-2
Name: T cell receptor beta, variable 13-2
Synonyms: Tcrb-V8.2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 100124675
Trav5n-4
Name: T cell receptor alpha variable 5N-4
Synonyms: ENSMUSG00000072521, Gm10903
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 100042614
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 14,910,774 bp
  • A to G, chromosome 1 at 46,175,438 bp
  • A to C, chromosome 1 at 88,138,868 bp
  • T to A, chromosome 2 at 32,454,836 bp
  • A to T, chromosome 2 at 58,151,123 bp
  • G to A, chromosome 2 at 69,265,512 bp
  • A to G, chromosome 2 at 82,984,902 bp
  • A to G, chromosome 2 at 84,938,327 bp
  • A to T, chromosome 2 at 88,412,251 bp
  • A to C, chromosome 2 at 89,609,947 bp
  • A to G, chromosome 2 at 124,660,312 bp
  • A to G, chromosome 2 at 129,101,144 bp
  • G to A, chromosome 2 at 158,761,366 bp
  • A to G, chromosome 2 at 178,348,295 bp
  • A to G, chromosome 3 at 89,409,108 bp
  • T to A, chromosome 3 at 135,324,590 bp
  • A to G, chromosome 4 at 41,767,539 bp
  • A to T, chromosome 4 at 88,755,236 bp
  • A to T, chromosome 4 at 96,641,423 bp
  • T to C, chromosome 4 at 119,187,158 bp
  • T to C, chromosome 4 at 132,745,180 bp
  • T to A, chromosome 4 at 141,957,613 bp
  • C to A, chromosome 5 at 91,957,538 bp
  • G to A, chromosome 5 at 104,072,090 bp
  • A to T, chromosome 5 at 108,798,096 bp
  • A to G, chromosome 6 at 41,121,782 bp
  • T to A, chromosome 6 at 125,123,522 bp
  • A to G, chromosome 7 at 15,626,223 bp
  • A to G, chromosome 7 at 29,166,661 bp
  • A to G, chromosome 7 at 44,218,572 bp
  • T to C, chromosome 7 at 111,073,920 bp
  • T to A, chromosome 8 at 60,516,086 bp
  • T to A, chromosome 8 at 61,329,996 bp
  • T to A, chromosome 8 at 70,581,681 bp
  • T to C, chromosome 8 at 72,723,398 bp
  • C to T, chromosome 9 at 52,126,558 bp
  • T to G, chromosome 9 at 71,849,815 bp
  • T to C, chromosome 9 at 85,724,155 bp
  • G to T, chromosome 10 at 34,394,828 bp
  • G to T, chromosome 10 at 81,386,025 bp
  • A to T, chromosome 10 at 127,742,946 bp
  • T to A, chromosome 10 at 128,085,500 bp
  • T to G, chromosome 11 at 3,680,013 bp
  • G to A, chromosome 11 at 8,865,550 bp
  • A to T, chromosome 11 at 28,434,146 bp
  • T to A, chromosome 11 at 33,555,023 bp
  • T to C, chromosome 11 at 50,156,751 bp
  • A to G, chromosome 11 at 55,268,303 bp
  • T to C, chromosome 11 at 59,061,068 bp
  • T to C, chromosome 11 at 70,978,487 bp
  • A to C, chromosome 11 at 75,567,053 bp
  • A to T, chromosome 11 at 103,497,524 bp
  • A to G, chromosome 11 at 104,852,545 bp
  • T to C, chromosome 11 at 120,819,118 bp
  • T to A, chromosome 12 at 10,381,450 bp
  • T to C, chromosome 12 at 76,057,484 bp
  • T to A, chromosome 12 at 80,947,554 bp
  • C to T, chromosome 12 at 84,853,741 bp
  • T to C, chromosome 12 at 86,881,722 bp
  • T to A, chromosome 12 at 87,773,360 bp
  • G to A, chromosome 12 at 108,854,387 bp
  • C to T, chromosome 14 at 19,891,370 bp
  • T to C, chromosome 14 at 53,312,905 bp
  • A to T, chromosome 14 at 103,169,994 bp
  • T to A, chromosome 15 at 34,277,502 bp
  • T to A, chromosome 15 at 54,260,112 bp
  • C to T, chromosome 15 at 76,175,331 bp
  • A to G, chromosome 16 at 91,010,083 bp
  • T to C, chromosome 17 at 20,624,292 bp
  • T to C, chromosome 17 at 26,441,773 bp
  • T to C, chromosome 17 at 32,675,988 bp
  • C to T, chromosome 17 at 33,003,030 bp
  • T to A, chromosome 17 at 86,488,197 bp
  • T to C, chromosome 18 at 61,157,649 bp
  • A to T, chromosome 19 at 23,265,694 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8711 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068565-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.