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Strain Name:
C57BL/6J-MtgxR8735Btlr/Mmmh
Stock Number:
068583-MU
Citation ID:
RRID:MMRRC_068583-MU
Other Names:
R8735 (G1)
Major Collection:

Strain Information

Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Suclg1
Name: succinate-CoA ligase, GDP-forming, alpha subunit
Synonyms: Sucla1, 1500000I01Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 56451
Homologene: 55785
Esrrg
Name: estrogen-related receptor gamma
Synonyms: estrogen-related receptor 3, ERR3, Errg, NR3B3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26381
HGNC: HGNC:3474
Homologene: 55581
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Nf1
Name: neurofibromin 1
Synonyms: neurofibromin, Nf-1, Dsk9, Mhdadsk9
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Trim36
Name: tripartite motif-containing 36
Synonyms: D18Wsu100e, Haprin
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 28105
VEGA: 18
Homologene: 10275
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 51,755,737 bp
  • C to T, chromosome 1 at 93,154,486 bp
  • A to G, chromosome 1 at 139,858,605 bp
  • A to T, chromosome 1 at 162,709,558 bp
  • G to T, chromosome 1 at 179,092,917 bp
  • A to G, chromosome 1 at 180,569,125 bp
  • A to T, chromosome 1 at 188,201,008 bp
  • T to C, chromosome 2 at 49,694,771 bp
  • T to C, chromosome 2 at 76,740,110 bp
  • A to G, chromosome 2 at 91,204,306 bp
  • T to A, chromosome 2 at 111,500,152 bp
  • G to A, chromosome 2 at 143,989,000 bp
  • A to G, chromosome 2 at 156,003,811 bp
  • T to C, chromosome 3 at 36,555,513 bp
  • T to C, chromosome 3 at 90,277,865 bp
  • T to A, chromosome 3 at 108,775,299 bp
  • T to A, chromosome 3 at 119,141,916 bp
  • T to G, chromosome 4 at 49,655,964 bp
  • T to A, chromosome 4 at 106,454,611 bp
  • G to A, chromosome 4 at 115,452,779 bp
  • A to T, chromosome 4 at 133,304,200 bp
  • A to G, chromosome 4 at 136,998,646 bp
  • T to C, chromosome 4 at 141,469,818 bp
  • T to A, chromosome 5 at 43,281,432 bp
  • T to A, chromosome 5 at 89,132,442 bp
  • T to C, chromosome 5 at 101,930,085 bp
  • A to T, chromosome 6 at 73,276,746 bp
  • C to T, chromosome 6 at 92,860,067 bp
  • A to C, chromosome 7 at 12,769,698 bp
  • C to T, chromosome 7 at 28,841,496 bp
  • C to T, chromosome 7 at 96,905,941 bp
  • GCG to GCGACGGCGACG, chromosome 7 at 97,579,907 bp
  • A to G, chromosome 7 at 139,910,214 bp
  • G to A, chromosome 7 at 140,072,590 bp
  • G to A, chromosome 8 at 61,372,653 bp
  • T to A, chromosome 8 at 71,889,183 bp
  • G to A, chromosome 9 at 37,408,281 bp
  • A to G, chromosome 9 at 49,077,561 bp
  • T to C, chromosome 9 at 70,034,446 bp
  • A to T, chromosome 9 at 103,210,524 bp
  • G to A, chromosome 9 at 108,564,779 bp
  • T to A, chromosome 10 at 27,190,534 bp
  • A to T, chromosome 10 at 69,986,955 bp
  • A to T, chromosome 11 at 35,883,407 bp
  • T to C, chromosome 11 at 49,217,400 bp
  • A to T, chromosome 11 at 60,510,853 bp
  • A to G, chromosome 11 at 79,454,310 bp
  • A to T, chromosome 11 at 83,186,944 bp
  • T to G, chromosome 11 at 83,283,889 bp
  • T to C, chromosome 11 at 87,147,059 bp
  • A to G, chromosome 11 at 97,052,368 bp
  • A to C, chromosome 11 at 101,276,266 bp
  • T to A, chromosome 12 at 4,671,474 bp
  • A to G, chromosome 12 at 44,283,439 bp
  • A to T, chromosome 12 at 115,507,597 bp
  • C to A, chromosome 13 at 11,686,947 bp
  • T to A, chromosome 13 at 22,383,367 bp
  • A to C, chromosome 13 at 30,906,839 bp
  • C to A, chromosome 13 at 46,727,551 bp
  • T to C, chromosome 13 at 67,671,431 bp
  • A to G, chromosome 13 at 92,274,866 bp
  • T to A, chromosome 14 at 103,223,150 bp
  • T to C, chromosome 15 at 76,356,575 bp
  • C to T, chromosome 16 at 17,318,370 bp
  • T to C, chromosome 16 at 17,635,546 bp
  • T to A, chromosome 16 at 30,581,047 bp
  • A to G, chromosome 16 at 45,737,634 bp
  • A to T, chromosome 16 at 73,958,359 bp
  • A to T, chromosome 16 at 79,001,814 bp
  • T to C, chromosome 17 at 14,883,667 bp
  • T to A, chromosome 17 at 25,745,088 bp
  • T to A, chromosome 18 at 36,968,150 bp
  • T to A, chromosome 18 at 37,356,922 bp
  • T to C, chromosome 18 at 46,169,385 bp
  • A to T, chromosome 18 at 61,063,977 bp
  • A to G, chromosome 18 at 74,217,260 bp
  • A to G, chromosome 19 at 12,900,910 bp
  • G to T, chromosome 19 at 44,301,304 bp
  • T to C, chromosome 19 at 46,671,094 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8735 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068583-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.