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Strain Name:
C57BL/6J-MtgxR8737Btlr/Mmmh
Stock Number:
068584-MU
Citation ID:
RRID:MMRRC_068584-MU
Other Names:
R8737 (G1)
Major Collection:

Strain Information

Btrc
Name: beta-transducin repeat containing protein
Synonyms: SCF b-TRCP, beta-TrCP, Slimb, Beta-Trcp1, Fbw1a
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12234
VEGA: 19
HGNC: HGNC:1144
Homologene: 39330
Met
Name: met proto-oncogene
Synonyms: HGF receptor, c-Met, Par4
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 17295
HGNC: HGNC:7029
Homologene: 206
Gad2
Name: glutamic acid decarboxylase 2
Synonyms: GAD65, Gad-2, 6330404F12Rik, GAD(65)
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14417
HGNC: HGNC:4093
Homologene: 20223
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Prrc1
Name: proline-rich coiled-coil 1
Synonyms: 2310058D16Rik, 9430085A19Rik, 3110038B19Rik, 1190002C06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 73137
VEGA: 18
Homologene: 12491
Pmpca
Name: peptidase (mitochondrial processing) alpha
Synonyms: INPP5E, Alpha-MPP, 4933435E07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66865
Homologene: 6078
Ino80e
Name: INO80 complex subunit E
Synonyms: Ccdc95
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233875
Homologene: 17792
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 24,185,046 bp
  • C to T, chromosome 1 at 34,228,669 bp
  • T to A, chromosome 1 at 38,269,729 bp
  • C to G, chromosome 1 at 82,893,522 bp
  • T to G, chromosome 1 at 90,800,025 bp
  • A to G, chromosome 1 at 134,040,305 bp
  • A to C, chromosome 1 at 173,277,689 bp
  • T to A, chromosome 1 at 178,864,865 bp
  • T to C, chromosome 1 at 193,348,486 bp
  • T to A, chromosome 2 at 22,634,973 bp
  • T to A, chromosome 2 at 26,393,519 bp
  • T to G, chromosome 2 at 30,878,566 bp
  • T to A, chromosome 2 at 36,479,617 bp
  • T to A, chromosome 2 at 62,512,433 bp
  • T to A, chromosome 2 at 85,494,043 bp
  • C to A, chromosome 2 at 89,002,007 bp
  • A to T, chromosome 2 at 93,421,894 bp
  • C to T, chromosome 2 at 94,376,029 bp
  • T to A, chromosome 2 at 120,269,985 bp
  • T to A, chromosome 2 at 121,025,843 bp
  • C to A, chromosome 3 at 62,479,326 bp
  • A to G, chromosome 4 at 6,396,748 bp
  • C to A, chromosome 4 at 134,767,836 bp
  • A to G, chromosome 4 at 141,583,076 bp
  • A to C, chromosome 4 at 144,208,622 bp
  • T to C, chromosome 4 at 155,610,585 bp
  • A to T, chromosome 5 at 121,482,370 bp
  • A to G, chromosome 5 at 136,282,942 bp
  • T to C, chromosome 6 at 17,540,511 bp
  • T to C, chromosome 6 at 18,319,729 bp
  • A to G, chromosome 6 at 65,456,276 bp
  • T to C, chromosome 6 at 73,067,445 bp
  • G to A, chromosome 6 at 125,162,054 bp
  • G to T, chromosome 7 at 13,335,229 bp
  • G to T, chromosome 7 at 47,235,876 bp
  • A to T, chromosome 7 at 85,406,575 bp
  • C to T, chromosome 7 at 96,905,941 bp
  • A to G, chromosome 7 at 103,906,706 bp
  • T to A, chromosome 7 at 108,713,757 bp
  • A to T, chromosome 7 at 126,861,802 bp
  • A to T, chromosome 7 at 135,713,775 bp
  • A to T, chromosome 8 at 109,733,598 bp
  • T to G, chromosome 9 at 22,678,948 bp
  • G to A, chromosome 9 at 110,627,881 bp
  • G to C, chromosome 11 at 29,154,815 bp
  • T to A, chromosome 11 at 49,294,138 bp
  • T to G, chromosome 11 at 59,983,459 bp
  • A to G, chromosome 12 at 3,999,594 bp
  • A to G, chromosome 12 at 8,967,665 bp
  • T to C, chromosome 13 at 3,642,089 bp
  • G to A, chromosome 13 at 95,665,750 bp
  • T to G, chromosome 14 at 34,017,192 bp
  • T to C, chromosome 14 at 41,134,087 bp
  • T to A, chromosome 14 at 51,456,265 bp
  • G to T, chromosome 14 at 65,645,086 bp
  • A to T, chromosome 15 at 55,455,310 bp
  • A to T, chromosome 15 at 57,892,172 bp
  • T to C, chromosome 15 at 76,708,854 bp
  • T to A, chromosome 15 at 102,521,767 bp
  • G to A, chromosome 17 at 23,310,168 bp
  • A to T, chromosome 17 at 32,798,223 bp
  • A to T, chromosome 17 at 45,632,875 bp
  • G to T, chromosome 17 at 80,550,829 bp
  • T to A, chromosome 18 at 57,363,336 bp
  • C to T, chromosome 18 at 61,081,001 bp
  • T to A, chromosome 19 at 5,118,449 bp
  • T to C, chromosome 19 at 45,507,759 bp
  • A to T, chromosome 19 at 46,669,727 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8737 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068584-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.