Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8772Btlr/Mmmh
Stock Number:
068626-MU
Citation ID:
RRID:MMRRC_068626-MU
Other Names:
R8772 (G1)
Major Collection:

Strain Information

Rptor
Name: regulatory associated protein of MTOR, complex 1
Synonyms: raptor, 4932417H02Rik, Rap
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74370
Homologene: 80210
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Lyst
Name: lysosomal trafficking regulator
Synonyms: D13Sfk13
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 17101
VEGA: 13
HGNC: HGNC:1968
Homologene: 61
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Atp1a1
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11928
HGNC: HGNC:799
Homologene: 564
Ubp1
Name: upstream binding protein 1
Synonyms: NF2d9, LBP-1b, LBP-1a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22221
VEGA: 9
Homologene: 8435
Eif2s2
Name: eukaryotic translation initiation factor 2 subunit 2 beta
Synonyms: EIF2B, EIF2, 2810026E11Rik, 38kDa, D2Ertd303e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67204
HGNC: HGNC:3266
Homologene: 2904
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 74,571,666 bp
  • A to G, chromosome 1 at 86,305,572 bp
  • A to G, chromosome 1 at 93,354,428 bp
  • C to G, chromosome 2 at 12,182,684 bp
  • T to A, chromosome 2 at 52,051,065 bp
  • T to A, chromosome 2 at 62,303,940 bp
  • T to A, chromosome 2 at 76,937,681 bp
  • T to C, chromosome 2 at 126,926,311 bp
  • T to C, chromosome 2 at 154,887,739 bp
  • A to G, chromosome 3 at 75,155,385 bp
  • G to A, chromosome 3 at 88,089,837 bp
  • A to T, chromosome 3 at 90,740,400 bp
  • A to C, chromosome 3 at 101,579,808 bp
  • T to C, chromosome 3 at 106,018,220 bp
  • A to T, chromosome 3 at 108,397,073 bp
  • A to G, chromosome 4 at 34,623,488 bp
  • A to T, chromosome 4 at 126,460,523 bp
  • T to A, chromosome 4 at 145,075,032 bp
  • G to A, chromosome 5 at 4,046,255 bp
  • A to T, chromosome 5 at 31,174,414 bp
  • G to T, chromosome 5 at 35,077,249 bp
  • A to T, chromosome 5 at 87,254,486 bp
  • A to T, chromosome 5 at 114,184,118 bp
  • T to A, chromosome 5 at 119,838,725 bp
  • T to C, chromosome 5 at 136,988,919 bp
  • T to C, chromosome 5 at 139,223,692 bp
  • T to A, chromosome 6 at 42,038,509 bp
  • T to A, chromosome 6 at 92,050,419 bp
  • T to A, chromosome 6 at 112,766,945 bp
  • A to T, chromosome 6 at 118,602,322 bp
  • T to A, chromosome 6 at 127,470,763 bp
  • T to A, chromosome 6 at 127,491,704 bp
  • C to T, chromosome 7 at 28,616,484 bp
  • C to A, chromosome 7 at 29,116,132 bp
  • A to T, chromosome 7 at 46,284,928 bp
  • CG to CGACGGCGGTG, chromosome 7 at 97,579,908 bp
  • G to A, chromosome 7 at 102,743,003 bp
  • A to G, chromosome 7 at 140,955,890 bp
  • T to C, chromosome 9 at 27,791,411 bp
  • G to A, chromosome 9 at 38,268,935 bp
  • T to A, chromosome 9 at 113,972,829 bp
  • T to A, chromosome 10 at 24,101,807 bp
  • A to G, chromosome 10 at 41,630,037 bp
  • T to A, chromosome 10 at 99,156,357 bp
  • T to C, chromosome 11 at 74,405,725 bp
  • C to T, chromosome 11 at 77,518,454 bp
  • T to G, chromosome 11 at 78,465,951 bp
  • A to G, chromosome 11 at 81,967,887 bp
  • A to G, chromosome 11 at 82,434,311 bp
  • A to G, chromosome 11 at 94,465,887 bp
  • A to C, chromosome 11 at 119,725,032 bp
  • A to T, chromosome 11 at 120,820,536 bp
  • T to C, chromosome 12 at 4,322,940 bp
  • T to C, chromosome 12 at 11,332,179 bp
  • C to T, chromosome 12 at 30,015,464 bp
  • A to T, chromosome 12 at 57,325,632 bp
  • A to C, chromosome 12 at 87,227,093 bp
  • T to C, chromosome 12 at 119,447,485 bp
  • A to G, chromosome 13 at 6,578,560 bp
  • C to T, chromosome 13 at 13,637,492 bp
  • T to A, chromosome 13 at 60,901,379 bp
  • T to C, chromosome 13 at 70,836,334 bp
  • T to A, chromosome 13 at 93,391,731 bp
  • G to A, chromosome 14 at 25,645,694 bp
  • A to T, chromosome 14 at 67,698,080 bp
  • T to C, chromosome 15 at 76,534,249 bp
  • C to T, chromosome 15 at 89,162,746 bp
  • T to C, chromosome 15 at 103,525,121 bp
  • A to T, chromosome 16 at 10,480,162 bp
  • G to A, chromosome 16 at 35,299,588 bp
  • A to G, chromosome 16 at 49,884,212 bp
  • A to G, chromosome 16 at 59,328,688 bp
  • C to T, chromosome 16 at 91,657,938 bp
  • T to A, chromosome 17 at 6,176,893 bp
  • T to A, chromosome 17 at 20,182,233 bp
  • T to C, chromosome 17 at 45,516,977 bp
  • T to A, chromosome 17 at 49,450,374 bp
  • A to G, chromosome 18 at 5,770,382 bp
  • C to T, chromosome 18 at 14,835,852 bp
  • G to C, chromosome 18 at 31,680,464 bp
  • T to C, chromosome 19 at 7,790,112 bp
  • A to G, chromosome 19 at 42,045,945 bp
  • A to G, chromosome 19 at 46,382,292 bp
  • A to T, chromosome 19 at 56,855,328 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8772 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068626-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.