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Strain Name:
C57BL/6J-MtgxR8849Btlr/Mmmh
Stock Number:
068672-MU
Citation ID:
RRID:MMRRC_068672-MU
Other Names:
R8849 (G1)
Major Collection:

Strain Information

Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20349
Homologene: 8247
Pax2
Name: paired box 2
Synonyms: Pax-2, Opdc
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18504
HGNC: HGNC:8616
Homologene: 2968
Phf20
Name: PHD finger protein 20
Synonyms: 6820402O20Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228829
Homologene: 9507
Kif20b
Name: kinesin family member 20B
Synonyms: N-6 kinesin, C330014J10Rik, Kif20b, Mphosph1, 33cex, magoo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 240641
VEGA: 19
HGNC: HGNC:7212
Homologene: 9418
Ercc6
Name: excision repair cross-complementing rodent repair deficiency, complementation group 6
Synonyms: CS group B correcting gene, CSB, C130058G22Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 319955
VEGA: 14
HGNC: HGNC:3438
Homologene: 133552
Ddx5
Name: DEAD box helicase 5
Synonyms: p68, Hlr1, 2600009A06Rik, DEAD (Asp-Glu-Ala-Asp) box polypeptide 5
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13207
HGNC: HGNC:2746
Homologene: 6797
Mms19
Name: MMS19 cytosolic iron-sulfur assembly component
Synonyms: 2610042O15Rik, Mms19, C86341, Mms19l
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 72199
Homologene: 41480
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 87,433,870 bp
  • T to C, chromosome 1 at 87,918,643 bp
  • G to A, chromosome 1 at 119,995,144 bp
  • G to A, chromosome 1 at 173,678,618 bp
  • C to T, chromosome 1 at 195,157,239 bp
  • T to C, chromosome 2 at 25,269,159 bp
  • T to C, chromosome 2 at 30,284,923 bp
  • T to A, chromosome 2 at 118,279,614 bp
  • G to T, chromosome 2 at 156,276,520 bp
  • T to C, chromosome 2 at 180,998,639 bp
  • A to G, chromosome 3 at 54,899,328 bp
  • A to G, chromosome 3 at 98,427,627 bp
  • A to C, chromosome 3 at 142,608,152 bp
  • T to A, chromosome 4 at 88,627,777 bp
  • C to T, chromosome 4 at 99,016,749 bp
  • A to G, chromosome 4 at 143,795,026 bp
  • T to C, chromosome 5 at 14,252,659 bp
  • A to T, chromosome 5 at 43,820,585 bp
  • A to T, chromosome 5 at 93,636,338 bp
  • T to A, chromosome 6 at 61,311,553 bp
  • T to C, chromosome 6 at 73,144,173 bp
  • T to C, chromosome 6 at 83,118,005 bp
  • T to C, chromosome 6 at 83,273,198 bp
  • T to C, chromosome 6 at 124,625,795 bp
  • G to T, chromosome 7 at 43,645,543 bp
  • C to A, chromosome 7 at 105,721,516 bp
  • T to A, chromosome 7 at 128,189,985 bp
  • C to T, chromosome 8 at 104,640,967 bp
  • T to A, chromosome 9 at 122,261,444 bp
  • T to A, chromosome 10 at 70,946,864 bp
  • T to A, chromosome 10 at 80,609,432 bp
  • T to C, chromosome 10 at 81,721,041 bp
  • T to A, chromosome 10 at 88,571,585 bp
  • T to C, chromosome 10 at 127,008,792 bp
  • T to A, chromosome 11 at 6,229,038 bp
  • A to T, chromosome 11 at 106,785,149 bp
  • T to A, chromosome 11 at 106,816,804 bp
  • T to C, chromosome 13 at 21,674,056 bp
  • T to A, chromosome 13 at 24,937,481 bp
  • T to C, chromosome 13 at 31,808,834 bp
  • T to C, chromosome 13 at 81,521,205 bp
  • C to T, chromosome 13 at 113,043,170 bp
  • T to A, chromosome 14 at 32,569,608 bp
  • A to G, chromosome 14 at 34,597,029 bp
  • A to T, chromosome 14 at 55,497,170 bp
  • C to T, chromosome 14 at 73,197,269 bp
  • T to C, chromosome 14 at 101,926,107 bp
  • G to A, chromosome 16 at 5,233,668 bp
  • G to A, chromosome 16 at 14,621,512 bp
  • T to A, chromosome 17 at 6,222,381 bp
  • A to G, chromosome 17 at 88,694,138 bp
  • T to A, chromosome 18 at 3,423,551 bp
  • C to T, chromosome 19 at 34,938,316 bp
  • A to T, chromosome 19 at 41,964,328 bp
  • T to C, chromosome 19 at 44,760,672 bp
  • T to C, chromosome 19 at 56,591,752 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8849 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068672-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.