Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8894Btlr/Mmmh
Stock Number:
068697-MU
Citation ID:
RRID:MMRRC_068697-MU
Other Names:
R8894 (G1)
Major Collection:

Strain Information

Sim1
Name: single-minded family bHLH transcription factor 1
Synonyms: bHLHe14
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20464
VEGA: 10
Homologene: 3715
Sort1
Name: sortilin 1
Synonyms: sortilin, neurotensin receptor 3, Ntsr3, Ntr3, 2900053A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Crybg3
Name: beta-gamma crystallin domain containing 3
Synonyms: Gm9581
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224273
Homologene: 28544
Plcd3
Name: phospholipase C, delta 3
Synonyms: 2610205J15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72469
HGNC: HGNC:9061
Homologene: 14858
Dhx35
Name: DEAH-box helicase 35
Synonyms: Ddx35, 1200009D07Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71715
Homologene: 6406
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 34,174,133 bp
  • A to G, chromosome 1 at 59,158,584 bp
  • T to A, chromosome 1 at 139,248,012 bp
  • T to A, chromosome 1 at 153,822,373 bp
  • T to C, chromosome 1 at 172,599,289 bp
  • A to T, chromosome 2 at 17,375,225 bp
  • T to G, chromosome 2 at 88,831,465 bp
  • G to C, chromosome 2 at 158,834,875 bp
  • T to A, chromosome 3 at 83,836,784 bp
  • C to T, chromosome 3 at 103,311,491 bp
  • T to C, chromosome 3 at 108,338,912 bp
  • A to G, chromosome 4 at 42,853,688 bp
  • A to T, chromosome 4 at 103,211,332 bp
  • T to C, chromosome 4 at 145,274,468 bp
  • T to G, chromosome 5 at 94,448,540 bp
  • T to C, chromosome 5 at 96,759,543 bp
  • G to T, chromosome 5 at 123,740,730 bp
  • T to A, chromosome 5 at 128,773,390 bp
  • T to C, chromosome 5 at 142,739,457 bp
  • A to C, chromosome 5 at 145,994,860 bp
  • A to G, chromosome 6 at 48,411,695 bp
  • T to C, chromosome 6 at 55,278,238 bp
  • A to G, chromosome 6 at 89,869,764 bp
  • A to T, chromosome 6 at 93,686,605 bp
  • T to A, chromosome 7 at 7,563,810 bp
  • T to C, chromosome 7 at 8,920,198 bp
  • G to A, chromosome 7 at 79,117,491 bp
  • A to T, chromosome 7 at 91,965,738 bp
  • A to C, chromosome 7 at 120,248,145 bp
  • T to G, chromosome 7 at 141,694,515 bp
  • C to T, chromosome 8 at 57,526,142 bp
  • C to A, chromosome 8 at 94,028,870 bp
  • A to T, chromosome 9 at 38,180,074 bp
  • C to T, chromosome 9 at 46,305,099 bp
  • T to C, chromosome 9 at 53,556,651 bp
  • A to T, chromosome 9 at 114,401,192 bp
  • T to C, chromosome 10 at 45,158,524 bp
  • T to A, chromosome 10 at 50,910,530 bp
  • T to A, chromosome 10 at 60,299,957 bp
  • T to C, chromosome 10 at 62,408,621 bp
  • T to A, chromosome 10 at 116,048,345 bp
  • T to A, chromosome 11 at 7,137,375 bp
  • T to A, chromosome 11 at 50,901,016 bp
  • T to A, chromosome 11 at 58,968,710 bp
  • T to A, chromosome 11 at 69,492,222 bp
  • T to C, chromosome 11 at 79,445,793 bp
  • T to C, chromosome 11 at 100,516,813 bp
  • T to A, chromosome 11 at 103,071,766 bp
  • T to C, chromosome 11 at 103,456,623 bp
  • T to C, chromosome 12 at 5,014,001 bp
  • A to G, chromosome 12 at 80,759,303 bp
  • T to C, chromosome 13 at 19,185,457 bp
  • T to G, chromosome 13 at 31,808,222 bp
  • A to T, chromosome 14 at 52,410,008 bp
  • T to C, chromosome 14 at 121,622,961 bp
  • A to T, chromosome 15 at 82,390,470 bp
  • T to C, chromosome 16 at 5,107,342 bp
  • T to A, chromosome 16 at 59,522,189 bp
  • T to A, chromosome 17 at 5,327,393 bp
  • C to T, chromosome 17 at 24,983,140 bp
  • T to C, chromosome 17 at 28,550,671 bp
  • T to C, chromosome 17 at 36,284,663 bp
  • T to A, chromosome 17 at 37,772,049 bp
  • A to G, chromosome 17 at 64,713,601 bp
  • C to T, chromosome 18 at 32,837,287 bp
  • T to C, chromosome 18 at 34,438,220 bp
  • A to T, chromosome 19 at 5,463,281 bp
  • G to A, chromosome 19 at 25,431,014 bp
  • A to T, chromosome 19 at 34,084,848 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8894 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068697-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.