Strain Name:
C57BL/6J-MtgxR8896Btlr/Mmmh
Stock Number:
068698-MU
Citation ID:
RRID:MMRRC_068698-MU
Other Names:
R8896 (G1)
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: GCKR, KHS, 4432415E19Rik, MAPKKKK5
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Herc4
Name: hect domain and RLD 4
Synonyms: 9530080M15Rik, 4921531D01Rik, 1700056O17Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67345
VEGA: 10
Homologene: 56715
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: Zfp406, LOC380993, Zfat1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 380993
Homologene: 16829
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Med30
Name: mediator complex subunit 30
Synonyms: Thrap6, Trap25, 1810038N03Rik, 2510044J04Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69790
VEGA: 15
Homologene: 12329
Nedd4l
Name: neural precursor cell expressed, developmentally down-regulated gene 4-like
Synonyms: Nedd4-2, Nedd4b, 1300012C07Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 83814
VEGA: 18
HGNC: HGNC:7728
Homologene: 86986
Kpna3
Name: karyopherin subunit alpha 3
Synonyms: IPOA4, importin alpha 4, importin alpha 4
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 16648
VEGA: 14
HGNC: HGNC:6396
Homologene: 20520
Mdn1
Name: midasin AAA ATPase 1
Synonyms: D4Abb1e, LOC213784, 4833432B22Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100019
Homologene: 39689
Lfng
Name: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms: lunatic fringe
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16848
HGNC: HGNC:6560
Homologene: 22475
Rarb
Name: retinoic acid receptor, beta
Synonyms: Hap, RAR beta 2, RARbeta2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218772
VEGA: 14
HGNC: HGNC:9865
Homologene: 68100
Dpp8
Name: dipeptidylpeptidase 8
Synonyms: 4932434F09Rik, 2310004I03Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74388
VEGA: 9
Homologene: 57098
Ap3d1
Name: adaptor-related protein complex 3, delta 1 subunit
Synonyms: mBLVR1, Bolvr
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 11776
VEGA: 10
HGNC: HGNC:568
Homologene: 2926
Galnt4
Name: polypeptide N-acetylgalactosaminyltransferase 4
Synonyms: ppGaNTase-T4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14426
Homologene: 74511
Efs
Name: embryonal Fyn-associated substrate
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 13644
VEGA: 14
Homologene: 4284
Fam193a
Name: family with sequence homology 193, member A
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231128
Homologene: 2746
Zfp738
Name: zinc finger protein 738
Synonyms: 3830402I07Rik, 6720487G11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 408068
Homologene: 133713
Kcnj9
Name: potassium inwardly-rectifying channel, subfamily J, member 9
Synonyms: 1700085N21Rik, Girk3, Kir3.3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16524
HGNC: HGNC:6270
Homologene: 37989
Oma1
Name: OMA1 zinc metallopeptidase
Synonyms: 2010001O09Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67013
Homologene: 12070
Hapln3
Name: hyaluronan and proteoglycan link protein 3
Synonyms: Lpr3, 4930554N11Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 67666
Homologene: 18654
Tmub1
Name: transmembrane and ubiquitin-like domain containing 1
Synonyms: Hops, 2010004O20Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 64295
Homologene: 11187
Glt8d2
Name: glycosyltransferase 8 domain containing 2
Synonyms: 1110021D20Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 74782
Homologene: 41826
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 99100
Homologene: 37159
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: tf, Itpr-3, Ip3r3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Trpv5
Name: transient receptor potential cation channel, subfamily V, member 5
Synonyms: CaT2, ECaC1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 194352
HGNC: HGNC:3145
Homologene: 10520
Dennd1c
Name: DENN domain containing 1C
Synonyms: 4432409M07Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 70785
VEGA: 17
Homologene: 11773
Dph2
Name: DPH2 homolog
Synonyms: 9130020C19Rik, Dph2l2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67728
HGNC: HGNC:3004
Homologene: 1060
Cdc42bpa
Name: CDC42 binding protein kinase alpha
Synonyms: A930014J19Rik, DMPK-like
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226751
HGNC: HGNC:1737
Homologene: 55765
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Spta1
Name: spectrin alpha, erythrocytic 1
Synonyms: Spna1, erythroid, ihj, Spna-1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 20739
Homologene: 74460
Wdr11
Name: WD repeat domain 11
Synonyms: 2900055P10Rik, Brwd2, Wdr11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 207425
Homologene: 41229
Zfp839
Name: zinc finger protein 839
Synonyms: 2810455K09Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 72805
VEGA: 12
Homologene: 49541
Gbp5
Name: guanylate binding protein 5
Synonyms: Gbp5a, 5330409J06Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229898
Homologene: 14183
Ryr3
Name: ryanodine receptor 3
Synonyms: calcium release channel isoform 3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20192
Homologene: 68151
Ccdc88b
Name: coiled-coil domain containing 88B
Synonyms: 2610041P08Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 78317
VEGA: 19
Homologene: 49992
Grm3
Name: glutamate receptor, metabotropic 3
Synonyms: 0710001G23Rik, Gprc1c, mGlu3, mGluR3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 108069
HGNC: HGNC:4595
Homologene: 651
Vmn2r5
Name: vomeronasal 2, receptor 5
Synonyms: EG667060
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 667060
Eml3
Name: echinoderm microtubule associated protein like 3
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225898
VEGA: 19
Homologene: 27044
Cyp2d40
Name: cytochrome P450, family 2, subfamily d, polypeptide 40
Synonyms: 1300013D18Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71754
VEGA: 15
Homologene: 135470
Myo3b
Name: myosin IIIB
Synonyms: A430065P19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329421
Homologene: 51393
Mppe1
Name: metallophosphoesterase 1
Synonyms: Pgap5
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225651
VEGA: 18
Homologene: 41512
Nod1
Name: nucleotide-binding oligomerization domain containing 1
Synonyms: Card4, Nlrc1, F830007N14Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 107607
Homologene: 4440
Nup210
Name: nucleoporin 210
Synonyms: gp190, gp210, Pom210
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 54563
Homologene: 41286
Megf6
Name: multiple EGF-like-domains 6
Synonyms: Egfl3, 2600001P17Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230971
HGNC: HGNC:3232
Homologene: 45412
Ces1c
Name: carboxylesterase 1C
Synonyms: Ces-N, Es-N, Es-1, Ee-1, Es1, Es-4
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 13884
HGNC: HGNC:1863
Homologene: 117484
Pkd1l2
Name: polycystic kidney disease 1 like 2
Synonyms: 1700126L06Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 76645
Homologene: 124481
Bean1
Name: brain expressed, associated with Nedd4, 1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 65115
Homologene: 110172
Aox4
Name: aldehyde oxidase 4
Synonyms: 2310003G12Rik, AOH2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 71872
Homologene: 70273
Atp11a
Name: ATPase, class VI, type 11A
Synonyms: Ih, 9130422H11Rik, LOC100045280, 4930558F19Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 50770
Homologene: 75050
Cadps2
Name: Ca2+-dependent activator protein for secretion 2
Synonyms: A230044C21Rik, Caps2, cpd2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320405
Homologene: 23060
Pla2g3
Name: phospholipase A2, group III
Synonyms: 9130003P18Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237625
Homologene: 9251
Or5ac20
Name: olfactory receptor family 5 subfamily AC member 20
Synonyms: Olfr202, MOR182-1, GA_x54KRFPKG5P-55498766-55497843
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258997
Homologene: 138323
Gbp2b
Name: guanylate binding protein 2b
Synonyms: LIMIT, Mag-1, Mpa1, Gbp-1, Gbp1, Mpa-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14468
Homologene: 137233
Prpsap2
Name: phosphoribosyl pyrophosphate synthetase-associated protein 2
Synonyms: A230054F23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 212627
HGNC: HGNC:9467
Homologene: 2073
Cr2
Name: complement receptor 2
Synonyms: Cr1, CD21, Cr-1, CD35, Cr-2, C3DR
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12902
HGNC: HGNC:2336
Homologene: 55611
Mindy4
Name: MINDY lysine 48 deubiquitinase 4
Synonyms: C330043M08Rik, LOC384387, Fam188b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330323
Homologene: 49991
Zer1
Name: zyg-11 related, cell cycle regulator
Synonyms: C230075L19Rik, Zyg11bl
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227693
Homologene: 4621
Stard13
Name: StAR related lipid transfer domain containing 13
Synonyms: DLC2, GT650
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243362
Homologene: 64844
Krt39
Name: keratin 39
Synonyms: 4732494G06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237934
Homologene: 19078
Atp4b
Name: ATPase, H+/K+ exchanging, beta polypeptide
Synonyms: H+/K+-ATPase beta, H,K-ATPase-Beta, H+,K+-ATPase
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11945
HGNC: HGNC:820
Homologene: 20182
Gstp3
Name: glutathione S-transferase pi 3
Synonyms: BC021614
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225884
HGNC: HGNC:4638
Homologene: 129928
Or8g36
Name: olfactory receptor family 8 subfamily G member 36
Synonyms: GA_x6K02T2PVTD-33208209-33207274, MOR171-12, Olfr957
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 258740
VEGA: 9
Ido1
Name: indoleamine 2,3-dioxygenase 1
Synonyms: Indo, Ido
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 15930
HGNC: HGNC:6059
Homologene: 48082
Fermt1
Name: fermitin family member 1
Synonyms: 5830467P10Rik, Kindlin-1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241639
Homologene: 9773
Oxsm
Name: 3-oxoacyl-ACP synthase, mitochondrial
Synonyms: 4933425A18Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71147
Homologene: 5820
Meltf
Name: melanotransferrin
Synonyms: MTf, Mfi2, melanotransferrin, CD228
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 30060
VEGA: 16
HGNC: HGNC:7037
Homologene: 4335
Pdcd5
Name: programmed cell death 5
Synonyms: Tfar19, 2200003D22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56330
HGNC: HGNC:8764
Homologene: 10506
Bcl11a
Name: BCL11 transcription factor A
Synonyms: Evi9, CTIP1, COUP-TF interacting protein 1, mouse myeloid leukemia gene, D930021L15Rik, 2810047E18Rik, Evi9a, Evi9b, Evi9c
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14025
Homologene: 11284
Or13p10
Name: olfactory receptor family 13 subfamily P member 10
Synonyms: MOR258-5, GA_x6K02T2QD9B-18877756-18876809, Olfr62, IH12
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18363
Dusp29
Name: dual specificity phosphatase 29
Synonyms: EG435391, Dupd1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 435391
VEGA: 14
Homologene: 51120
Glrx
Name: glutaredoxin
Synonyms: Grx1, D13Wsu156e, TTase
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 93692
HGNC: HGNC:4330
Homologene: 37566
Lrit3
Name: leucine-rich repeat, immunoglobulin-like and transmembrane domains 3
Synonyms: LOC242235
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242235
Homologene: 19426
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 58,252,074 bp
  • T to A, chromosome 1 at 172,325,793 bp
  • T to C, chromosome 1 at 174,217,982 bp
  • GACAACA to GACA, chromosome 1 at 180,130,808 bp
  • A to T, chromosome 1 at 195,169,273 bp
  • A to G, chromosome 2 at 30,103,418 bp
  • C to A, chromosome 2 at 70,238,816 bp
  • A to T, chromosome 2 at 112,753,050 bp
  • A to T, chromosome 2 at 125,566,235 bp
  • C to T, chromosome 2 at 132,941,932 bp
  • G to A, chromosome 3 at 64,503,782 bp
  • A to G, chromosome 3 at 90,118,625 bp
  • T to A, chromosome 3 at 129,791,483 bp
  • C to T, chromosome 3 at 142,505,547 bp
  • C to A, chromosome 3 at 142,603,566 bp
  • C to T, chromosome 4 at 32,678,328 bp
  • T to C, chromosome 4 at 103,353,632 bp
  • G to A, chromosome 4 at 117,890,378 bp
  • A to T, chromosome 4 at 118,666,305 bp
  • T to A, chromosome 4 at 154,242,403 bp
  • A to G, chromosome 5 at 9,512,483 bp
  • A to T, chromosome 5 at 24,446,682 bp
  • A to G, chromosome 5 at 34,426,484 bp
  • G to A, chromosome 5 at 140,613,223 bp
  • G to A, chromosome 5 at 151,062,650 bp
  • T to A, chromosome 6 at 23,410,877 bp
  • A to G, chromosome 6 at 41,670,913 bp
  • A to G, chromosome 6 at 54,944,292 bp
  • T to C, chromosome 6 at 55,278,238 bp
  • A to T, chromosome 6 at 91,042,253 bp
  • A to T, chromosome 7 at 35,647,370 bp
  • G to A, chromosome 7 at 79,117,491 bp
  • C to T, chromosome 7 at 129,605,713 bp
  • T to C, chromosome 8 at 12,849,781 bp
  • T to C, chromosome 8 at 13,387,514 bp
  • G to T, chromosome 8 at 24,587,864 bp
  • T to C, chromosome 8 at 93,106,626 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • T to C, chromosome 8 at 117,013,876 bp
  • T to G, chromosome 8 at 120,576,446 bp
  • A to G, chromosome 9 at 39,511,474 bp
  • A to C, chromosome 9 at 65,078,036 bp
  • T to A, chromosome 10 at 63,311,507 bp
  • T to A, chromosome 10 at 80,716,591 bp
  • T to A, chromosome 10 at 82,654,782 bp
  • T to A, chromosome 10 at 83,570,018 bp
  • T to A, chromosome 10 at 99,110,093 bp
  • A to T, chromosome 11 at 3,492,151 bp
  • A to G, chromosome 11 at 24,163,640 bp
  • A to G, chromosome 11 at 61,752,910 bp
  • A to T, chromosome 11 at 99,518,269 bp
  • T to A, chromosome 12 at 69,823,501 bp
  • C to T, chromosome 12 at 110,868,843 bp
  • G to A, chromosome 13 at 67,669,791 bp
  • G to T, chromosome 13 at 75,847,198 bp
  • A to T, chromosome 14 at 16,242,677 bp
  • A to G, chromosome 14 at 16,436,804 bp
  • T to C, chromosome 14 at 21,686,695 bp
  • T to C, chromosome 14 at 54,920,299 bp
  • T to C, chromosome 14 at 61,391,845 bp
  • G to A, chromosome 15 at 52,721,120 bp
  • A to T, chromosome 15 at 68,180,670 bp
  • A to G, chromosome 15 at 76,194,360 bp
  • T to C, chromosome 15 at 82,760,253 bp
  • T to A, chromosome 16 at 31,890,704 bp
  • C to T, chromosome 16 at 32,754,673 bp
  • A to T, chromosome 16 at 59,284,089 bp
  • G to A, chromosome 17 at 27,118,677 bp
  • T to A, chromosome 17 at 57,074,512 bp
  • A to G, chromosome 18 at 65,165,617 bp
  • T to C, chromosome 18 at 67,237,401 bp
  • A to T, chromosome 19 at 4,058,573 bp
  • C to T, chromosome 19 at 6,853,835 bp
  • A to G, chromosome 19 at 8,936,692 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8896 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068698-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.