Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR8896Btlr/Mmmh
Stock Number:
068698-MU
Citation ID:
RRID:MMRRC_068698-MU
Other Names:
R8896 (G1)
Major Collection:

Strain Information

Map4k5
Name: mitogen-activated protein kinase kinase kinase kinase 5
Synonyms: MAPKKKK5, GCKR, KHS, 4432415E19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 399510
HGNC: HGNC:6867
Homologene: 38199
Nup210l
Name: nucleoporin 210-like
Synonyms: 4930548O11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 77595
Homologene: 28122
Herc4
Name: hect domain and RLD 4
Synonyms: 4921531D01Rik, 1700056O17Rik, 9530080M15Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 67345
VEGA: 10
Homologene: 56715
Washc4
Name: WASH complex subunit 4
Synonyms: A230046K03Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 319277
VEGA: 10
Homologene: 123926
Zfat
Name: zinc finger and AT hook domain containing
Synonyms: LOC380993, Zfp406, Zfat1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 380993
Homologene: 16829
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Med30
Name: mediator complex subunit 30
Synonyms: 2510044J04Rik, 1810038N03Rik, Trap25, Thrap6
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 69790
VEGA: 15
Homologene: 12329
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 58,252,074 bp
  • T to A, chromosome 1 at 172,325,793 bp
  • T to C, chromosome 1 at 174,217,982 bp
  • GACAACA to GACA, chromosome 1 at 180,130,808 bp
  • A to T, chromosome 1 at 195,169,273 bp
  • A to G, chromosome 2 at 30,103,418 bp
  • C to A, chromosome 2 at 70,238,816 bp
  • A to T, chromosome 2 at 112,753,050 bp
  • A to T, chromosome 2 at 125,566,235 bp
  • C to T, chromosome 2 at 132,941,932 bp
  • G to A, chromosome 3 at 64,503,782 bp
  • A to G, chromosome 3 at 90,118,625 bp
  • T to A, chromosome 3 at 129,791,483 bp
  • C to T, chromosome 3 at 142,505,547 bp
  • C to A, chromosome 3 at 142,603,566 bp
  • C to T, chromosome 4 at 32,678,328 bp
  • T to C, chromosome 4 at 103,353,632 bp
  • G to A, chromosome 4 at 117,890,378 bp
  • A to T, chromosome 4 at 118,666,305 bp
  • T to A, chromosome 4 at 154,242,403 bp
  • A to G, chromosome 5 at 9,512,483 bp
  • A to T, chromosome 5 at 24,446,682 bp
  • A to G, chromosome 5 at 34,426,484 bp
  • G to A, chromosome 5 at 140,613,223 bp
  • G to A, chromosome 5 at 151,062,650 bp
  • T to A, chromosome 6 at 23,410,877 bp
  • A to G, chromosome 6 at 41,670,913 bp
  • A to G, chromosome 6 at 54,944,292 bp
  • T to C, chromosome 6 at 55,278,238 bp
  • A to T, chromosome 6 at 91,042,253 bp
  • A to T, chromosome 7 at 35,647,370 bp
  • G to A, chromosome 7 at 79,117,491 bp
  • C to T, chromosome 7 at 129,605,713 bp
  • T to C, chromosome 8 at 12,849,781 bp
  • T to C, chromosome 8 at 13,387,514 bp
  • G to T, chromosome 8 at 24,587,864 bp
  • T to C, chromosome 8 at 93,106,626 bp
  • CT to C, chromosome 8 at 104,182,032 bp
  • T to C, chromosome 8 at 117,013,876 bp
  • T to G, chromosome 8 at 120,576,446 bp
  • A to G, chromosome 9 at 39,511,474 bp
  • A to C, chromosome 9 at 65,078,036 bp
  • T to A, chromosome 10 at 63,311,507 bp
  • T to A, chromosome 10 at 80,716,591 bp
  • T to A, chromosome 10 at 82,654,782 bp
  • T to A, chromosome 10 at 83,570,018 bp
  • T to A, chromosome 10 at 99,110,093 bp
  • A to T, chromosome 11 at 3,492,151 bp
  • A to G, chromosome 11 at 24,163,640 bp
  • A to G, chromosome 11 at 61,752,910 bp
  • A to T, chromosome 11 at 99,518,269 bp
  • T to A, chromosome 12 at 69,823,501 bp
  • C to T, chromosome 12 at 110,868,843 bp
  • G to A, chromosome 13 at 67,669,791 bp
  • G to T, chromosome 13 at 75,847,198 bp
  • A to T, chromosome 14 at 16,242,677 bp
  • A to G, chromosome 14 at 16,436,804 bp
  • T to C, chromosome 14 at 21,686,695 bp
  • T to C, chromosome 14 at 54,920,299 bp
  • T to C, chromosome 14 at 61,391,845 bp
  • G to A, chromosome 15 at 52,721,120 bp
  • A to T, chromosome 15 at 68,180,670 bp
  • A to G, chromosome 15 at 76,194,360 bp
  • T to C, chromosome 15 at 82,760,253 bp
  • T to A, chromosome 16 at 31,890,704 bp
  • C to T, chromosome 16 at 32,754,673 bp
  • A to T, chromosome 16 at 59,284,089 bp
  • G to A, chromosome 17 at 27,118,677 bp
  • T to A, chromosome 17 at 57,074,512 bp
  • A to G, chromosome 18 at 65,165,617 bp
  • T to C, chromosome 18 at 67,237,401 bp
  • A to T, chromosome 19 at 4,058,573 bp
  • C to T, chromosome 19 at 6,853,835 bp
  • A to G, chromosome 19 at 8,936,692 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8896 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068698-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.