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Strain Name:
C57BL/6J-MtgxR8919Btlr/Mmmh
Stock Number:
068706-MU
Citation ID:
RRID:MMRRC_068706-MU
Other Names:
R8919 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Atp1a1
Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms: Atpa-1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 11928
HGNC: HGNC:799
Homologene: 564
Ptprk
Name: protein tyrosine phosphatase receptor type K
Synonyms: PTPk, RPTPkappa
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 19272
VEGA: 10
HGNC: HGNC:9674
Homologene: 55693
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649
Fubp3
Name: far upstream element (FUSE) binding protein 3
Synonyms: FBP3, A330051M14Rik, Marta2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 320267
HGNC: HGNC:4005
Homologene: 45954
Ptp4a2
Name: protein tyrosine phosphatase 4a2
Synonyms: Prl-2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 19244
HGNC: HGNC:9635
Homologene: 20744
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 80,505,430 bp
  • A to G, chromosome 1 at 91,516,074 bp
  • A to G, chromosome 1 at 121,499,241 bp
  • T to C, chromosome 1 at 173,216,497 bp
  • T to C, chromosome 1 at 173,928,928 bp
  • G to A, chromosome 2 at 10,098,011 bp
  • A to G, chromosome 2 at 31,592,464 bp
  • T to C, chromosome 2 at 32,214,941 bp
  • C to T, chromosome 2 at 52,237,129 bp
  • A to G, chromosome 2 at 66,337,986 bp
  • A to C, chromosome 2 at 87,672,223 bp
  • G to A, chromosome 2 at 122,301,876 bp
  • A to G, chromosome 3 at 32,953,184 bp
  • T to C, chromosome 3 at 55,150,947 bp
  • A to C, chromosome 3 at 62,531,057 bp
  • T to C, chromosome 3 at 92,317,725 bp
  • C to A, chromosome 3 at 94,957,164 bp
  • T to C, chromosome 3 at 101,591,231 bp
  • T to C, chromosome 3 at 131,295,465 bp
  • G to A, chromosome 4 at 3,184,863 bp
  • A to G, chromosome 4 at 72,158,288 bp
  • G to A, chromosome 4 at 129,845,152 bp
  • C to T, chromosome 4 at 145,223,580 bp
  • A to G, chromosome 5 at 67,976,956 bp
  • G to T, chromosome 5 at 125,029,189 bp
  • T to A, chromosome 5 at 144,883,612 bp
  • A to G, chromosome 6 at 24,108,195 bp
  • C to T, chromosome 6 at 55,497,095 bp
  • A to G, chromosome 6 at 58,684,341 bp
  • A to G, chromosome 6 at 86,445,792 bp
  • T to C, chromosome 6 at 128,334,003 bp
  • A to T, chromosome 7 at 6,305,257 bp
  • A to C, chromosome 7 at 26,611,781 bp
  • A to T, chromosome 7 at 67,619,085 bp
  • T to A, chromosome 7 at 102,604,504 bp
  • T to C, chromosome 7 at 105,388,270 bp
  • A to G, chromosome 7 at 108,287,082 bp
  • C to A, chromosome 7 at 144,411,528 bp
  • C to T, chromosome 8 at 60,540,124 bp
  • C to T, chromosome 8 at 80,981,850 bp
  • T to A, chromosome 8 at 107,368,596 bp
  • T to C, chromosome 8 at 121,944,780 bp
  • A to G, chromosome 9 at 15,326,711 bp
  • A to G, chromosome 9 at 26,999,489 bp
  • A to G, chromosome 9 at 57,529,046 bp
  • A to G, chromosome 9 at 83,839,269 bp
  • G to T, chromosome 9 at 122,797,865 bp
  • T to A, chromosome 10 at 28,483,207 bp
  • G to A, chromosome 10 at 59,441,646 bp
  • A to T, chromosome 10 at 70,004,841 bp
  • T to C, chromosome 10 at 88,110,327 bp
  • T to A, chromosome 10 at 93,857,913 bp
  • G to T, chromosome 10 at 129,410,213 bp
  • G to T, chromosome 11 at 3,923,569 bp
  • T to C, chromosome 11 at 9,291,653 bp
  • T to C, chromosome 11 at 73,136,604 bp
  • T to C, chromosome 11 at 100,141,154 bp
  • C to A, chromosome 11 at 101,166,399 bp
  • T to C, chromosome 11 at 119,299,932 bp
  • A to G, chromosome 12 at 23,779,886 bp
  • T to A, chromosome 12 at 87,259,084 bp
  • T to C, chromosome 13 at 22,806,053 bp
  • A to T, chromosome 13 at 49,068,235 bp
  • G to T, chromosome 13 at 69,503,709 bp
  • G to A, chromosome 13 at 95,015,112 bp
  • T to C, chromosome 14 at 32,172,891 bp
  • A to G, chromosome 14 at 51,162,890 bp
  • A to T, chromosome 14 at 55,512,647 bp
  • A to T, chromosome 14 at 123,323,872 bp
  • C to A, chromosome 15 at 6,435,790 bp
  • C to T, chromosome 15 at 76,497,851 bp
  • G to A, chromosome 15 at 99,671,945 bp
  • A to T, chromosome 15 at 100,814,540 bp
  • A to C, chromosome 16 at 29,605,522 bp
  • T to A, chromosome 16 at 87,381,493 bp
  • T to C, chromosome 16 at 88,812,173 bp
  • G to A, chromosome 17 at 25,857,172 bp
  • A to G, chromosome 17 at 33,001,654 bp
  • A to G, chromosome 17 at 35,847,951 bp
  • A to C, chromosome 17 at 56,882,218 bp
  • T to C, chromosome 18 at 56,592,465 bp
  • T to A, chromosome 19 at 36,845,688 bp
  • T to A, chromosome 19 at 45,076,956 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8919 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068706-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.