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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8991Btlr/Mmmh
Stock Number:
068716-MU
Citation ID:
RRID:MMRRC_068716-MU
Other Names:
R8991 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Synonyms: Kir1.2, BIRK-1, BIR10, Kir4.1, Kir4.1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16513
HGNC: HGNC:6256
Homologene: 1689

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Fn1
Name: fibronectin 1
Synonyms: Fn-1, Fn
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 14268
HGNC: HGNC:3778
Homologene: 1533

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Kcnmb4
Name: potassium large conductance calcium-activated channel, subfamily M, beta member 4
Synonyms: Slowpoke beta 4, 2900045G12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 58802
HGNC: HGNC:6289
Homologene: 8721

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Prmt7
Name: protein arginine N-methyltransferase 7
Synonyms: 4933402B05Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 214572
Homologene: 10382

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mtf2
Name: metal response element binding transcription factor 2
Synonyms: M96, 9230112N11Rik, C76717, Pcl2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17765
Homologene: 7207

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Cc2d1b
Name: coiled-coil and C2 domain containing 1B
Synonyms: A830039B04Rik, Freud2
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 319965
Homologene: 70951

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 71,637,332 bp
  • T to A, chromosome 1 at 172,369,396 bp
  • A to G, chromosome 2 at 66,684,244 bp
  • T to C, chromosome 2 at 82,985,026 bp
  • T to C, chromosome 2 at 85,063,946 bp
  • A to C, chromosome 2 at 88,412,379 bp
  • T to C, chromosome 2 at 111,293,003 bp
  • A to T, chromosome 2 at 112,092,337 bp
  • T to G, chromosome 3 at 83,128,836 bp
  • A to T, chromosome 4 at 108,624,946 bp
  • C to T, chromosome 5 at 14,669,311 bp
  • G to A, chromosome 5 at 34,905,718 bp
  • T to C, chromosome 5 at 103,870,538 bp
  • T to A, chromosome 5 at 108,100,939 bp
  • A to T, chromosome 5 at 109,086,167 bp
  • T to C, chromosome 5 at 121,358,284 bp
  • C to T, chromosome 6 at 28,434,473 bp
  • T to A, chromosome 6 at 41,312,361 bp
  • A to T, chromosome 7 at 6,424,721 bp
  • T to A, chromosome 7 at 23,952,651 bp
  • T to A, chromosome 7 at 25,289,460 bp
  • A to T, chromosome 7 at 30,359,819 bp
  • A to T, chromosome 7 at 80,677,443 bp
  • G to A, chromosome 7 at 101,580,383 bp
  • A to T, chromosome 7 at 102,563,701 bp
  • T to C, chromosome 7 at 110,312,689 bp
  • A to G, chromosome 7 at 141,243,758 bp
  • C to A, chromosome 7 at 141,433,958 bp
  • G to A, chromosome 8 at 88,129,819 bp
  • G to T, chromosome 8 at 92,360,507 bp
  • A to G, chromosome 8 at 106,217,242 bp
  • T to G, chromosome 8 at 116,952,926 bp
  • T to A, chromosome 10 at 116,446,333 bp
  • A to T, chromosome 11 at 29,173,306 bp
  • A to T, chromosome 11 at 65,886,680 bp
  • A to G, chromosome 11 at 79,045,188 bp
  • C to A, chromosome 11 at 105,338,914 bp
  • A to G, chromosome 11 at 106,019,145 bp
  • T to C, chromosome 12 at 46,817,017 bp
  • T to C, chromosome 12 at 91,234,213 bp
  • T to G, chromosome 12 at 114,253,646 bp
  • T to A, chromosome 13 at 40,142,801 bp
  • T to A, chromosome 13 at 92,439,217 bp
  • A to T, chromosome 15 at 8,291,513 bp
  • G to C, chromosome 15 at 33,213,207 bp
  • T to A, chromosome 16 at 19,605,761 bp
  • A to G, chromosome 16 at 48,873,675 bp
  • G to T, chromosome 16 at 91,656,478 bp
  • C to T, chromosome 16 at 91,656,720 bp
  • A to C, chromosome 17 at 23,310,312 bp
  • A to C, chromosome 17 at 28,164,053 bp
  • T to A, chromosome 17 at 34,840,190 bp
  • G to A, chromosome 17 at 43,085,408 bp
  • C to T, chromosome 17 at 57,081,169 bp
  • A to T, chromosome 18 at 6,620,232 bp
  • C to A, chromosome 18 at 13,846,080 bp
  • T to A, chromosome 18 at 58,106,323 bp
  • T to C, chromosome 19 at 25,188,367 bp
  • T to C, chromosome 19 at 41,938,026 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8991 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068716-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.