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Strain Detail Sheet

Strain Name:
C57BL/6J-MtgxR8845Btlr/Mmmh
Stock Number:
068734-MU
Citation ID:
RRID:MMRRC_068734-MU
Other Names:
R8845 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Vps33a
Name: VPS33A CORVET/HOPS core subunit
Synonyms: bf, 3830421M04Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 77573
Homologene: 11294

Additional Resources
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Gene Ontology
BioGPS
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KOMP Phenotyping
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ClinGen
Sigmar1
Name: sigma non-opioid intracellular receptor 1
Synonyms: mSigmaR1, Oprs1, Sig1R
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18391
HGNC: HGNC:8157
Homologene: 39965

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227723
Homologene: 106649

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Cyfip1
Name: cytoplasmic FMR1 interacting protein 1
Synonyms: P140SRA-1, Shyc, pl-1, l(7)1Rl, Sra-1, E030028J09Rik, l7Rl1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20430
Homologene: 22628

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Bnc2
Name: basonuclin zinc finger protein 2
Synonyms: 5031434M05Rik, 8430420F16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242509
Homologene: 18243

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Gphn
Name: gephyrin
Synonyms: geph, 5730552E08Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 268566
VEGA: 12
Homologene: 10820

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Fnbp4
Name: formin binding protein 4
Synonyms: FBP30
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 55935
Homologene: 9087

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 125,981,686 bp
  • T to A, chromosome 2 at 32,212,093 bp
  • A to T, chromosome 2 at 32,216,150 bp
  • A to G, chromosome 2 at 60,428,709 bp
  • T to C, chromosome 2 at 76,776,036 bp
  • A to G, chromosome 2 at 76,802,314 bp
  • G to T, chromosome 2 at 87,857,201 bp
  • T to A, chromosome 2 at 88,683,391 bp
  • A to G, chromosome 2 at 90,776,024 bp
  • A to T, chromosome 3 at 75,894,965 bp
  • G to A, chromosome 3 at 122,137,002 bp
  • CGGC to CGGCGGGGGC, chromosome 3 at 134,240,151 bp
  • G to A, chromosome 4 at 41,741,234 bp
  • C to T, chromosome 4 at 84,276,101 bp
  • T to C, chromosome 4 at 100,953,146 bp
  • A to C, chromosome 4 at 115,458,296 bp
  • T to C, chromosome 5 at 33,882,541 bp
  • T to C, chromosome 5 at 100,711,382 bp
  • T to C, chromosome 5 at 111,224,175 bp
  • C to T, chromosome 5 at 123,144,247 bp
  • A to T, chromosome 5 at 123,571,475 bp
  • C to A, chromosome 5 at 123,954,407 bp
  • T to A, chromosome 5 at 144,173,886 bp
  • T to C, chromosome 5 at 150,543,382 bp
  • G to A, chromosome 6 at 3,504,926 bp
  • T to C, chromosome 6 at 83,358,172 bp
  • A to T, chromosome 6 at 131,630,679 bp
  • G to T, chromosome 7 at 55,930,086 bp
  • A to G, chromosome 7 at 98,261,625 bp
  • A to T, chromosome 7 at 103,383,150 bp
  • C to T, chromosome 7 at 120,247,205 bp
  • A to T, chromosome 7 at 128,252,518 bp
  • T to C, chromosome 9 at 65,558,681 bp
  • G to T, chromosome 9 at 109,194,765 bp
  • G to A, chromosome 10 at 40,841,794 bp
  • A to G, chromosome 10 at 80,170,097 bp
  • A to G, chromosome 10 at 129,388,196 bp
  • C to A, chromosome 11 at 70,247,051 bp
  • A to T, chromosome 11 at 105,008,961 bp
  • T to C, chromosome 11 at 108,570,367 bp
  • A to G, chromosome 12 at 4,851,439 bp
  • A to G, chromosome 12 at 8,298,905 bp
  • T to C, chromosome 12 at 37,244,365 bp
  • A to G, chromosome 12 at 78,492,179 bp
  • A to T, chromosome 12 at 112,920,094 bp
  • C to T, chromosome 13 at 22,524,550 bp
  • G to T, chromosome 13 at 81,481,359 bp
  • A to T, chromosome 13 at 95,657,884 bp
  • T to C, chromosome 14 at 34,536,677 bp
  • T to C, chromosome 14 at 53,091,389 bp
  • C to T, chromosome 14 at 103,688,311 bp
  • T to G, chromosome 15 at 44,505,254 bp
  • A to G, chromosome 15 at 58,126,136 bp
  • G to A, chromosome 15 at 95,654,636 bp
  • G to A, chromosome 15 at 96,695,019 bp
  • A to C, chromosome 16 at 32,756,515 bp
  • A to T, chromosome 17 at 20,471,099 bp
  • C to A, chromosome 17 at 25,171,164 bp
  • A to T, chromosome 17 at 25,946,849 bp
  • A to C, chromosome 17 at 48,414,231 bp
  • A to T, chromosome 18 at 21,097,322 bp
  • A to G, chromosome 18 at 25,132,919 bp
  • T to A, chromosome 18 at 32,861,045 bp
  • C to T, chromosome 18 at 44,275,539 bp
  • A to T, chromosome 19 at 5,054,919 bp
  • A to C, chromosome 19 at 12,764,300 bp
  • A to T, chromosome 19 at 23,724,577 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8845 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068734-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.