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Strain Name:
C57BL/6J-MtgxR8901Btlr/Mmmh
Stock Number:
068758-MU
Citation ID:
RRID:MMRRC_068758-MU
Other Names:
R8901 (G1)
Major Collection:

Strain Information

Chrna5
Name: cholinergic receptor, nicotinic, alpha polypeptide 5
Synonyms: Acra-5, Acra5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110835
VEGA: 9
HGNC: HGNC:1959
Homologene: 55485
Scn3a
Name: sodium channel, voltage-gated, type III, alpha
Synonyms: LOC381367, Nav1.3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20269
Homologene: 56005
Sntb2
Name: syntrophin, basic 2
Synonyms: Snt2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 20650
Homologene: 4911
Gtf2i
Name: general transcription factor II I
Synonyms: TFII-I, BAP-135, 6030441I21Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14886
HGNC: HGNC:4659
Homologene: 7748
Rlf
Name: rearranged L-myc fusion sequence
Synonyms: 9230110M18Rik, MommeD8
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 109263
Homologene: 8243
Wdr43
Name: WD repeat domain 43
Synonyms: 2610318G08Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 72515
VEGA: 17
Homologene: 38810
Cenpf
Name: centromere protein F
Synonyms: 6530404A22Rik, Lek1, mitosin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108000
HGNC: HGNC:1857
Homologene: 22969
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 72,585,780 bp
  • A to C, chromosome 1 at 83,205,922 bp
  • C to T, chromosome 1 at 162,546,245 bp
  • C to T, chromosome 1 at 189,662,051 bp
  • C to A, chromosome 2 at 60,502,056 bp
  • C to T, chromosome 2 at 65,521,908 bp
  • A to T, chromosome 2 at 120,102,324 bp
  • T to A, chromosome 2 at 129,125,675 bp
  • T to A, chromosome 3 at 84,445,567 bp
  • C to A, chromosome 3 at 96,601,429 bp
  • T to C, chromosome 3 at 138,957,544 bp
  • T to C, chromosome 4 at 119,281,588 bp
  • T to C, chromosome 4 at 121,146,813 bp
  • A to T, chromosome 4 at 143,617,107 bp
  • G to A, chromosome 4 at 146,897,714 bp
  • CTTT to C, chromosome 5 at 25,950,859 bp
  • T to C, chromosome 5 at 30,891,091 bp
  • T to C, chromosome 5 at 63,806,342 bp
  • T to A, chromosome 5 at 72,700,707 bp
  • T to C, chromosome 5 at 123,161,051 bp
  • A to G, chromosome 5 at 134,295,535 bp
  • T to A, chromosome 5 at 140,712,853 bp
  • T to A, chromosome 6 at 131,689,951 bp
  • G to A, chromosome 7 at 35,632,818 bp
  • T to A, chromosome 7 at 45,341,717 bp
  • T to C, chromosome 8 at 45,248,814 bp
  • A to G, chromosome 8 at 70,194,149 bp
  • C to T, chromosome 8 at 88,663,809 bp
  • C to T, chromosome 8 at 93,210,475 bp
  • T to C, chromosome 8 at 93,968,059 bp
  • T to C, chromosome 8 at 107,011,343 bp
  • A to T, chromosome 8 at 121,730,822 bp
  • T to C, chromosome 9 at 55,004,453 bp
  • A to G, chromosome 9 at 107,534,794 bp
  • T to G, chromosome 10 at 95,989,569 bp
  • A to T, chromosome 11 at 49,470,208 bp
  • T to C, chromosome 11 at 67,041,738 bp
  • A to T, chromosome 11 at 102,460,804 bp
  • A to T, chromosome 12 at 104,780,952 bp
  • G to T, chromosome 12 at 112,976,600 bp
  • G to A, chromosome 13 at 38,181,179 bp
  • G to T, chromosome 14 at 34,226,071 bp
  • T to C, chromosome 14 at 54,570,373 bp
  • T to C, chromosome 14 at 55,886,586 bp
  • T to G, chromosome 15 at 28,365,569 bp
  • T to A, chromosome 15 at 66,685,335 bp
  • C to T, chromosome 15 at 88,933,943 bp
  • A to T, chromosome 16 at 56,252,165 bp
  • A to T, chromosome 17 at 34,043,280 bp
  • A to T, chromosome 17 at 36,187,360 bp
  • T to A, chromosome 17 at 58,330,161 bp
  • A to T, chromosome 17 at 71,625,466 bp
  • A to T, chromosome 17 at 88,755,602 bp
  • C to A, chromosome 18 at 32,846,960 bp
  • T to G, chromosome 19 at 8,824,445 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8901 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068758-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.