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Strain Name:
C57BL/6J-MtgxR8911Btlr/Mmmh
Stock Number:
068764-MU
Citation ID:
RRID:MMRRC_068764-MU
Other Names:
R8911 (G1)
Major Collection:

Strain Information

Lhx5
Name: LIM homeobox protein 5
Synonyms: Lim2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 16873
Homologene: 40621
Agt
Name: angiotensinogen
Synonyms: Aogen, Serpina8, angiotensin precursor
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11606
HGNC: HGNC:333
Homologene: 14
Pik3cb
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Synonyms: 1110001J02Rik, p110beta
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 74769
HGNC: HGNC:8976
Homologene: 21250
Abca12
Name: ATP-binding cassette, sub-family A member 12
Synonyms: 4832428G11Rik, 4833417A11Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 74591
Homologene: 45441
Upf1
Name: UPF1 RNA helicase and ATPase
Synonyms: PNORF-1, B430202H16Rik, Rent1
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 19704
HGNC: HGNC:9962
Homologene: 2185
Usp48
Name: ubiquitin specific peptidase 48
Synonyms: D330022K21Rik, 2810449C13Rik, Usp31
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 170707
Homologene: 12988
Usp14
Name: ubiquitin specific peptidase 14
Synonyms: dUB-type TGT, 2610005K12Rik, ataxia, ax, NMF375, 2610037B11Rik, nmf375
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 59025
Homologene: 3780
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 40,543,017 bp
  • T to C, chromosome 1 at 71,341,531 bp
  • T to A, chromosome 1 at 133,638,601 bp
  • A to T, chromosome 1 at 153,152,127 bp
  • T to C, chromosome 1 at 162,920,545 bp
  • C to T, chromosome 1 at 186,721,444 bp
  • A to G, chromosome 2 at 5,983,082 bp
  • A to T, chromosome 2 at 11,482,443 bp
  • T to C, chromosome 2 at 27,997,618 bp
  • C to A, chromosome 2 at 70,476,618 bp
  • T to C, chromosome 2 at 89,023,950 bp
  • T to C, chromosome 2 at 89,125,264 bp
  • T to C, chromosome 2 at 144,559,396 bp
  • A to G, chromosome 2 at 154,009,170 bp
  • C to T, chromosome 2 at 164,952,648 bp
  • C to T, chromosome 2 at 173,095,328 bp
  • C to A, chromosome 2 at 181,238,380 bp
  • A to G, chromosome 3 at 23,846,593 bp
  • T to C, chromosome 3 at 75,906,396 bp
  • A to T, chromosome 3 at 97,743,601 bp
  • G to T, chromosome 3 at 107,604,287 bp
  • T to C, chromosome 3 at 148,852,527 bp
  • G to A, chromosome 4 at 11,317,078 bp
  • A to T, chromosome 4 at 119,437,089 bp
  • T to A, chromosome 4 at 131,776,249 bp
  • G to A, chromosome 4 at 137,613,685 bp
  • T to A, chromosome 5 at 120,436,444 bp
  • C to A, chromosome 6 at 3,516,710 bp
  • T to C, chromosome 6 at 106,353,782 bp
  • T to C, chromosome 6 at 129,275,224 bp
  • C to G, chromosome 7 at 26,266,885 bp
  • A to T, chromosome 7 at 44,943,270 bp
  • G to A, chromosome 7 at 62,379,789 bp
  • A to G, chromosome 7 at 122,992,045 bp
  • A to G, chromosome 7 at 137,128,065 bp
  • T to C, chromosome 8 at 16,698,003 bp
  • A to T, chromosome 8 at 70,338,437 bp
  • T to C, chromosome 8 at 124,564,445 bp
  • G to A, chromosome 9 at 40,911,157 bp
  • A to G, chromosome 9 at 99,064,148 bp
  • A to G, chromosome 9 at 106,890,262 bp
  • C to G, chromosome 9 at 108,611,739 bp
  • A to G, chromosome 9 at 120,441,418 bp
  • T to C, chromosome 10 at 68,541,754 bp
  • T to G, chromosome 10 at 76,387,525 bp
  • C to T, chromosome 10 at 81,487,685 bp
  • A to G, chromosome 10 at 127,876,943 bp
  • C to T, chromosome 11 at 24,164,763 bp
  • A to T, chromosome 11 at 53,955,983 bp
  • A to G, chromosome 11 at 73,113,621 bp
  • C to T, chromosome 11 at 87,206,803 bp
  • A to G, chromosome 11 at 99,772,738 bp
  • G to A, chromosome 12 at 16,690,902 bp
  • A to G, chromosome 12 at 31,833,239 bp
  • A to G, chromosome 12 at 32,197,258 bp
  • A to G, chromosome 12 at 51,748,833 bp
  • A to T, chromosome 12 at 54,692,655 bp
  • A to T, chromosome 13 at 67,619,942 bp
  • C to T, chromosome 13 at 70,592,668 bp
  • A to T, chromosome 13 at 77,281,115 bp
  • T to C, chromosome 14 at 13,823,796 bp
  • A to T, chromosome 14 at 32,661,669 bp
  • A to G, chromosome 14 at 55,887,278 bp
  • C to T, chromosome 15 at 97,796,908 bp
  • T to G, chromosome 16 at 33,738,257 bp
  • A to T, chromosome 16 at 90,978,734 bp
  • A to T, chromosome 17 at 38,335,429 bp
  • A to G, chromosome 17 at 47,389,119 bp
  • G to T, chromosome 17 at 50,608,113 bp
  • C to T, chromosome 17 at 56,423,320 bp
  • A to C, chromosome 17 at 58,199,048 bp
  • A to G, chromosome 18 at 9,996,194 bp
  • C to A, chromosome 18 at 20,451,872 bp
  • A to T, chromosome 18 at 37,767,065 bp
  • G to A, chromosome 19 at 4,150,804 bp
  • A to C, chromosome 19 at 40,913,426 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8911 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068764-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.