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Strain Name:
C57BL/6J-MtgxR8930Btlr/Mmmh
Stock Number:
068774-MU
Citation ID:
RRID:MMRRC_068774-MU
Other Names:
R8930 (G1)
Major Collection:

Strain Information

Cdh1
Name: cadherin 1
Synonyms: E-cadherin, Ecad, UM, uvomorulin, L-CAM, E-cad
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 12550
HGNC: HGNC:1748
Homologene: 20917
Kdm5b
Name: lysine demethylase 5B
Synonyms: PLU-1, 2010009J12Rik, Plu1, Rb-Bp2, 2210016I17Rik, D1Ertd202e, Jarid1b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 75605
Homologene: 48448
Wnk1
Name: WNK lysine deficient protein kinase 1
Synonyms: 6430573H23Rik, Prkwnk1, EG406236, Hsn2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232341
Homologene: 14253
Abl2
Name: ABL proto-oncogene 2, non-receptor tyrosine kinase
Synonyms: Arg, Abll
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11352
HGNC: HGNC:77
Homologene: 5278
Dyrk3
Name: dual-specificity tyrosine phosphorylation regulated kinase 3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226419
HGNC: HGNC:3094
Homologene: 55762
Pdxdc1
Name: pyridoxal-dependent decarboxylase domain containing 1
Synonyms: 2210010A19Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 94184
Homologene: 22858
Srfbp1
Name: serum response factor binding protein 1
Synonyms: 2810036K01Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67222
VEGA: 18
Homologene: 12101
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,935,808 bp
  • G to A, chromosome 1 at 70,299,769 bp
  • G to T, chromosome 1 at 131,129,556 bp
  • C to T, chromosome 1 at 134,616,272 bp
  • A to G, chromosome 1 at 156,633,832 bp
  • A to T, chromosome 1 at 159,912,789 bp
  • T to A, chromosome 2 at 31,492,375 bp
  • C to T, chromosome 2 at 67,482,363 bp
  • C to T, chromosome 2 at 91,939,570 bp
  • T to C, chromosome 2 at 121,024,286 bp
  • A to G, chromosome 2 at 172,375,703 bp
  • G to T, chromosome 3 at 107,472,191 bp
  • T to A, chromosome 4 at 60,580,709 bp
  • C to T, chromosome 5 at 14,712,975 bp
  • T to G, chromosome 5 at 93,638,085 bp
  • GAT to GATCAT, chromosome 6 at 4,756,449 bp
  • T to A, chromosome 6 at 57,071,681 bp
  • T to C, chromosome 6 at 57,844,013 bp
  • T to C, chromosome 6 at 87,821,531 bp
  • A to T, chromosome 6 at 119,963,265 bp
  • T to C, chromosome 6 at 121,765,071 bp
  • T to C, chromosome 6 at 124,317,923 bp
  • A to G, chromosome 7 at 63,757,491 bp
  • T to C, chromosome 7 at 80,282,493 bp
  • T to A, chromosome 7 at 81,167,967 bp
  • C to T, chromosome 7 at 82,611,861 bp
  • G to A, chromosome 7 at 101,408,117 bp
  • C to T, chromosome 7 at 101,901,143 bp
  • C to T, chromosome 7 at 121,902,844 bp
  • A to G, chromosome 7 at 127,908,010 bp
  • A to G, chromosome 7 at 134,899,152 bp
  • A to G, chromosome 7 at 135,698,899 bp
  • T to C, chromosome 7 at 140,037,529 bp
  • T to C, chromosome 8 at 21,735,357 bp
  • A to G, chromosome 8 at 41,209,831 bp
  • A to T, chromosome 8 at 70,781,733 bp
  • A to G, chromosome 8 at 85,095,393 bp
  • ACTCGAAATGATGTGGCTC to ACTC, chromosome 8 at 106,666,238 bp
  • C to T, chromosome 8 at 122,895,979 bp
  • G to A, chromosome 9 at 15,999,523 bp
  • A to T, chromosome 9 at 79,673,383 bp
  • T to C, chromosome 9 at 82,906,988 bp
  • G to T, chromosome 9 at 96,686,381 bp
  • A to T, chromosome 10 at 7,160,780 bp
  • C to A, chromosome 10 at 81,144,534 bp
  • A to G, chromosome 11 at 30,892,571 bp
  • T to C, chromosome 11 at 34,402,707 bp
  • A to G, chromosome 11 at 79,943,229 bp
  • C to T, chromosome 13 at 46,674,392 bp
  • C to T, chromosome 13 at 59,742,201 bp
  • T to A, chromosome 13 at 68,583,716 bp
  • C to T, chromosome 13 at 118,387,159 bp
  • G to A, chromosome 15 at 34,453,698 bp
  • A to G, chromosome 15 at 102,990,886 bp
  • A to T, chromosome 16 at 13,854,405 bp
  • T to A, chromosome 16 at 78,373,410 bp
  • A to G, chromosome 17 at 21,560,430 bp
  • T to C, chromosome 17 at 28,364,494 bp
  • C to T, chromosome 17 at 34,594,589 bp
  • A to G, chromosome 17 at 55,997,895 bp
  • C to T, chromosome 17 at 66,956,851 bp
  • C to T, chromosome 18 at 3,123,127 bp
  • T to C, chromosome 18 at 6,629,693 bp
  • T to A, chromosome 18 at 20,539,661 bp
  • T to A, chromosome 18 at 52,490,045 bp
  • T to C, chromosome 18 at 67,775,064 bp
  • T to A, chromosome 19 at 5,135,002 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8930 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068774-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.