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Strain Name:
C57BL/6J-MtgxR8936Btlr/Mmmh
Stock Number:
068779-MU
Citation ID:
RRID:MMRRC_068779-MU
Other Names:
R8936 (G1)
Major Collection:

Strain Information

Myh7
Name: myosin, heavy polypeptide 7, cardiac muscle, beta
Synonyms: Myhc-b, Myhcb, beta-MHC, B-MHC, MYH-beta/slow, MyHC-I, betaMHC
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 140781
HGNC: HGNC:7577
Homologene: 68044
Ntrk1
Name: neurotrophic tyrosine kinase, receptor, type 1
Synonyms: TrkA, Tkr
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18211
HGNC: HGNC:8031
Homologene: 1898
Tet1
Name: tet methylcytosine dioxygenase 1
Synonyms: 2510010B09Rik, D10Ertd17e, Cxxc6, BB001228
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52463
Homologene: 12735
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Nudt5
Name: nudix hydrolase 5
Synonyms: nudix (nucleoside diphosphate linked moiety X)-type motif 5
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 53893
HGNC: HGNC:8052
Homologene: 5388
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 65,271,268 bp
  • G to A, chromosome 1 at 173,929,291 bp
  • T to G, chromosome 2 at 5,864,417 bp
  • A to C, chromosome 2 at 25,195,190 bp
  • G to C, chromosome 2 at 26,062,204 bp
  • G to A, chromosome 2 at 37,603,937 bp
  • A to T, chromosome 2 at 85,063,976 bp
  • T to A, chromosome 2 at 109,333,621 bp
  • A to G, chromosome 2 at 110,637,110 bp
  • A to T, chromosome 2 at 119,964,228 bp
  • A to G, chromosome 2 at 145,949,959 bp
  • A to G, chromosome 2 at 160,748,066 bp
  • A to G, chromosome 2 at 180,661,402 bp
  • G to A, chromosome 2 at 180,738,417 bp
  • G to T, chromosome 2 at 181,691,265 bp
  • T to A, chromosome 3 at 69,018,158 bp
  • A to T, chromosome 3 at 87,786,059 bp
  • A to G, chromosome 3 at 106,504,605 bp
  • T to C, chromosome 3 at 121,775,468 bp
  • A to G, chromosome 3 at 129,332,235 bp
  • A to T, chromosome 4 at 94,501,095 bp
  • A to T, chromosome 4 at 108,252,159 bp
  • A to G, chromosome 4 at 123,117,045 bp
  • G to T, chromosome 4 at 148,497,333 bp
  • A to G, chromosome 5 at 27,721,142 bp
  • C to T, chromosome 5 at 106,635,637 bp
  • C to T, chromosome 5 at 127,792,612 bp
  • T to A, chromosome 5 at 137,525,207 bp
  • T to A, chromosome 5 at 145,286,390 bp
  • T to C, chromosome 5 at 150,661,897 bp
  • C to A, chromosome 6 at 52,163,537 bp
  • T to A, chromosome 6 at 58,472,098 bp
  • T to C, chromosome 6 at 90,223,589 bp
  • A to T, chromosome 6 at 106,715,643 bp
  • T to A, chromosome 6 at 122,851,085 bp
  • T to A, chromosome 7 at 19,521,950 bp
  • T to C, chromosome 7 at 41,864,557 bp
  • T to C, chromosome 7 at 80,381,725 bp
  • T to C, chromosome 7 at 99,459,992 bp
  • A to G, chromosome 7 at 130,626,637 bp
  • G to T, chromosome 8 at 13,045,086 bp
  • A to G, chromosome 8 at 13,065,319 bp
  • A to T, chromosome 8 at 34,853,347 bp
  • G to A, chromosome 8 at 43,651,402 bp
  • T to A, chromosome 8 at 71,607,663 bp
  • C to T, chromosome 8 at 104,628,590 bp
  • A to G, chromosome 8 at 122,895,101 bp
  • A to G, chromosome 8 at 125,088,015 bp
  • G to A, chromosome 9 at 35,053,427 bp
  • A to G, chromosome 9 at 51,099,998 bp
  • T to C, chromosome 9 at 123,040,476 bp
  • A to T, chromosome 9 at 123,963,845 bp
  • A to T, chromosome 10 at 62,840,284 bp
  • T to A, chromosome 10 at 75,414,993 bp
  • T to A, chromosome 10 at 80,712,118 bp
  • T to A, chromosome 10 at 88,558,575 bp
  • C to T, chromosome 10 at 129,011,933 bp
  • A to G, chromosome 11 at 51,254,422 bp
  • A to T, chromosome 11 at 59,002,032 bp
  • C to A, chromosome 11 at 106,567,318 bp
  • A to T, chromosome 12 at 17,416,862 bp
  • A to C, chromosome 13 at 34,128,462 bp
  • T to C, chromosome 13 at 34,911,164 bp
  • G to A, chromosome 14 at 20,471,427 bp
  • G to A, chromosome 14 at 49,770,567 bp
  • A to G, chromosome 14 at 50,186,542 bp
  • A to T, chromosome 14 at 51,418,526 bp
  • T to G, chromosome 14 at 54,990,983 bp
  • T to A, chromosome 14 at 67,845,990 bp
  • T to A, chromosome 15 at 16,831,076 bp
  • A to G, chromosome 15 at 44,538,916 bp
  • T to C, chromosome 15 at 76,668,519 bp
  • A to T, chromosome 15 at 101,053,458 bp
  • T to A, chromosome 16 at 17,213,020 bp
  • C to T, chromosome 17 at 14,001,675 bp
  • T to A, chromosome 17 at 24,422,202 bp
  • T to A, chromosome 17 at 34,685,672 bp
  • T to A, chromosome 17 at 46,528,602 bp
  • C to A, chromosome 18 at 24,121,808 bp
  • A to G, chromosome 18 at 61,523,877 bp
  • A to G, chromosome 19 at 4,787,511 bp
  • G to A, chromosome 19 at 11,914,361 bp
  • A to G, chromosome 19 at 17,121,835 bp
  • G to T, chromosome 19 at 33,580,480 bp
  • A to G, chromosome 19 at 43,808,662 bp
  • T to C, chromosome Y at 738,726 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8936 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068779-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.