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Strain Name:
C57BL/6J-MtgxR8944Btlr/Mmmh
Stock Number:
068783-MU
Citation ID:
RRID:MMRRC_068783-MU
Other Names:
R8944 (G1)
Major Collection:

Strain Information

Myh9
Name: myosin, heavy polypeptide 9, non-muscle
Synonyms: D0Jmb2, E030044M24Rik, NMHC II-A, Myhn-1, Myhn1, myosin IIA, Fltn
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 17886
HGNC: HGNC:7579
Homologene: 129835
Cd74
Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Synonyms: CLIP, Ii
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 16149
VEGA: 18
HGNC: HGNC:1697
Homologene: 3209
Lamb3
Name: laminin, beta 3
Synonyms: nicein, 125kDa
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16780
HGNC: HGNC:6490
Homologene: 191
Mta3
Name: metastasis associated 3
Synonyms: 1110002J22Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 116871
Homologene: 14282
Lrp2
Name: low density lipoprotein receptor-related protein 2
Synonyms: Megalin, Gp330, D230004K18Rik, b2b1625.2Clo
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 14725
HGNC: HGNC:6694
Homologene: 20952
Ppcdc
Name: phosphopantothenoylcysteine decarboxylase
Synonyms: 8430432M10Rik, 1810057I13Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66812
VEGA: 9
Homologene: 11049
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik, nemitin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99512
Homologene: 8984
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 11,414,482 bp
  • A to G, chromosome 1 at 24,728,326 bp
  • T to C, chromosome 1 at 28,777,074 bp
  • T to A, chromosome 1 at 83,279,206 bp
  • G to T, chromosome 1 at 87,192,275 bp
  • T to A, chromosome 1 at 91,359,452 bp
  • A to G, chromosome 1 at 117,727,350 bp
  • A to T, chromosome 1 at 161,241,862 bp
  • T to A, chromosome 1 at 193,332,217 bp
  • A to C, chromosome 2 at 35,100,610 bp
  • C to T, chromosome 2 at 69,511,004 bp
  • G to T, chromosome 2 at 76,792,840 bp
  • G to A, chromosome 2 at 105,127,239 bp
  • G to A, chromosome 2 at 120,986,835 bp
  • A to G, chromosome 2 at 144,491,809 bp
  • G to T, chromosome 2 at 180,277,976 bp
  • C to T, chromosome 3 at 58,629,854 bp
  • A to T, chromosome 3 at 73,700,675 bp
  • A to T, chromosome 3 at 100,149,943 bp
  • T to A, chromosome 3 at 108,643,164 bp
  • G to C, chromosome 3 at 154,757,055 bp
  • G to A, chromosome 4 at 94,621,891 bp
  • A to G, chromosome 4 at 98,941,006 bp
  • T to C, chromosome 5 at 87,001,451 bp
  • C to A, chromosome 5 at 87,325,558 bp
  • T to A, chromosome 5 at 114,131,395 bp
  • A to G, chromosome 5 at 121,414,510 bp
  • A to G, chromosome 5 at 124,568,725 bp
  • T to C, chromosome 5 at 143,380,505 bp
  • G to T, chromosome 6 at 57,361,157 bp
  • C to A, chromosome 6 at 58,307,289 bp
  • G to T, chromosome 6 at 124,855,233 bp
  • T to C, chromosome 6 at 134,644,474 bp
  • T to C, chromosome 6 at 145,225,127 bp
  • G to A, chromosome 7 at 15,902,975 bp
  • A to G, chromosome 7 at 30,922,576 bp
  • G to A, chromosome 7 at 79,710,257 bp
  • T to A, chromosome 7 at 97,534,386 bp
  • A to T, chromosome 7 at 141,867,378 bp
  • G to T, chromosome 7 at 143,881,165 bp
  • A to G, chromosome 7 at 144,070,190 bp
  • A to G, chromosome 8 at 24,674,687 bp
  • A to T, chromosome 8 at 34,807,787 bp
  • T to A, chromosome 8 at 78,435,303 bp
  • T to A, chromosome 8 at 105,690,805 bp
  • G to A, chromosome 9 at 49,520,193 bp
  • C to T, chromosome 9 at 57,434,982 bp
  • A to G, chromosome 9 at 75,004,280 bp
  • A to T, chromosome 10 at 63,065,869 bp
  • T to A, chromosome 10 at 95,996,960 bp
  • T to A, chromosome 10 at 102,545,468 bp
  • T to A, chromosome 10 at 115,347,766 bp
  • T to C, chromosome 10 at 120,473,254 bp
  • G to T, chromosome 10 at 127,010,586 bp
  • T to C, chromosome 11 at 49,148,439 bp
  • A to T, chromosome 11 at 58,786,693 bp
  • A to T, chromosome 11 at 72,159,025 bp
  • C to T, chromosome 11 at 80,095,698 bp
  • T to A, chromosome 11 at 114,750,476 bp
  • C to T, chromosome 11 at 116,175,214 bp
  • A to G, chromosome 11 at 121,359,558 bp
  • T to A, chromosome 12 at 29,811,565 bp
  • T to A, chromosome 12 at 31,340,869 bp
  • A to T, chromosome 12 at 57,643,040 bp
  • T to G, chromosome 12 at 80,576,540 bp
  • A to G, chromosome 12 at 113,056,856 bp
  • T to C, chromosome 12 at 114,456,083 bp
  • T to G, chromosome 12 at 118,127,646 bp
  • A to T, chromosome 13 at 33,833,278 bp
  • A to T, chromosome 14 at 44,656,392 bp
  • T to C, chromosome 14 at 47,267,013 bp
  • T to C, chromosome 14 at 55,675,692 bp
  • T to G, chromosome 14 at 69,032,435 bp
  • T to A, chromosome 15 at 33,594,123 bp
  • A to G, chromosome 15 at 39,138,217 bp
  • T to C, chromosome 15 at 77,771,232 bp
  • T to C, chromosome 15 at 101,528,748 bp
  • G to A, chromosome 16 at 32,497,682 bp
  • T to A, chromosome 16 at 94,501,622 bp
  • T to C, chromosome 17 at 34,742,939 bp
  • T to C, chromosome 17 at 34,971,043 bp
  • T to A, chromosome 17 at 35,930,126 bp
  • G to T, chromosome 17 at 56,993,553 bp
  • A to C, chromosome 17 at 65,970,200 bp
  • G to A, chromosome 17 at 83,775,717 bp
  • G to T, chromosome 18 at 34,842,876 bp
  • T to A, chromosome 18 at 56,743,259 bp
  • T to C, chromosome 18 at 60,810,055 bp
  • T to C, chromosome 19 at 17,474,911 bp
  • A to G, chromosome 19 at 38,911,066 bp
  • T to C, chromosome 19 at 47,611,618 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8944 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068783-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.