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Strain Detail Sheet

Published:
Revised: 03/02/2024

Strain Name:
C57BL/6J-MtgxR8951Btlr/Mmmh
Stock Number:
068788-MU
Citation ID:
RRID:MMRRC_068788-MU
Other Names:
R8951 (G1)
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Rgs8
Name: regulator of G-protein signaling 8
Synonyms: 6530413N01Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 67792
Homologene: 23152

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Slc1a2
Name: solute carrier family 1 (glial high affinity glutamate transporter), member 2
Synonyms: GLT1, MGLT1, Eaat2, 1700091C19Rik, GLT-1, 2900019G14Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20511
Homologene: 3075

Additional Resources
IMPC
Google
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Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
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Xrn1
Name: 5'-3' exoribonuclease 1
Synonyms: mXrn1, Dhm2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 24127
Homologene: 5894

Additional Resources
IMPC
Google
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IMSR
Gene Ontology
BioGPS
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Mouse Mine
KOMP Phenotyping
Monarch
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ClinGen
Lhx9
Name: LIM homeobox protein 9
Synonyms: LH2B, Lhx9 alpha, 3110009O07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 16876
Homologene: 7816

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
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Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
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Polr1f
Name: RNA polymerase I subunit F
Synonyms: D16Wsu83e, 2810024J17Rik, Twistnb
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 28071
VEGA: 12
Homologene: 45411

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Ipo11
Name: importin 11
Synonyms: E330021B14Rik, 1700081H05Rik, 2510001A17Rik, Ranbp11
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76582
Homologene: 7089

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Dhdds
Name: dehydrodolichyl diphosphate synthase
Synonyms: 3222401G21Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 67422
Homologene: 32615

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 36,518,938 bp
  • G to A, chromosome 1 at 85,246,950 bp
  • T to A, chromosome 1 at 87,188,027 bp
  • A to G, chromosome 1 at 138,841,966 bp
  • A to T, chromosome 1 at 153,670,821 bp
  • A to G, chromosome 1 at 182,157,744 bp
  • A to C, chromosome 2 at 44,789,018 bp
  • C to T, chromosome 2 at 88,323,631 bp
  • T to C, chromosome 2 at 102,756,008 bp
  • T to C, chromosome 2 at 151,970,541 bp
  • T to A, chromosome 3 at 89,445,557 bp
  • T to A, chromosome 3 at 142,500,720 bp
  • A to T, chromosome 4 at 41,719,845 bp
  • A to T, chromosome 4 at 51,229,469 bp
  • T to G, chromosome 4 at 63,273,074 bp
  • T to A, chromosome 4 at 120,267,936 bp
  • C to T, chromosome 4 at 133,992,546 bp
  • T to A, chromosome 5 at 11,042,832 bp
  • A to C, chromosome 5 at 24,537,954 bp
  • A to G, chromosome 5 at 33,259,335 bp
  • A to T, chromosome 5 at 73,671,229 bp
  • T to G, chromosome 5 at 120,862,594 bp
  • T to C, chromosome 5 at 140,689,823 bp
  • T to C, chromosome 6 at 43,081,704 bp
  • T to C, chromosome 6 at 118,613,339 bp
  • T to A, chromosome 6 at 131,685,606 bp
  • A to T, chromosome 6 at 145,220,612 bp
  • G to A, chromosome 7 at 4,149,783 bp
  • C to T, chromosome 7 at 44,858,193 bp
  • TCCCAGG to T, chromosome 7 at 80,098,331 bp
  • ATGGCG to ATGGCGACGGTGGCG, chromosome 7 at 97,579,904 bp
  • G to A, chromosome 7 at 100,998,021 bp
  • A to G, chromosome 7 at 105,180,431 bp
  • A to G, chromosome 7 at 118,721,792 bp
  • A to G, chromosome 7 at 119,500,388 bp
  • A to T, chromosome 7 at 119,718,248 bp
  • A to G, chromosome 8 at 69,793,834 bp
  • T to C, chromosome 8 at 84,903,283 bp
  • T to C, chromosome 8 at 90,347,915 bp
  • T to C, chromosome 8 at 95,034,734 bp
  • T to C, chromosome 8 at 111,914,065 bp
  • T to C, chromosome 8 at 116,911,165 bp
  • A to G, chromosome 9 at 65,272,938 bp
  • A to G, chromosome 9 at 95,988,946 bp
  • T to C, chromosome 9 at 100,880,801 bp
  • C to T, chromosome 9 at 108,294,509 bp
  • C to T, chromosome 10 at 117,676,033 bp
  • T to A, chromosome 10 at 120,038,604 bp
  • T to C, chromosome 11 at 58,219,582 bp
  • T to C, chromosome 11 at 95,054,085 bp
  • T to C, chromosome 11 at 102,774,367 bp
  • T to A, chromosome 12 at 8,952,172 bp
  • G to T, chromosome 12 at 33,437,868 bp
  • A to C, chromosome 12 at 108,346,089 bp
  • A to G, chromosome 12 at 111,445,876 bp
  • T to A, chromosome 12 at 113,259,064 bp
  • T to A, chromosome 12 at 113,307,306 bp
  • T to A, chromosome 13 at 56,543,009 bp
  • T to C, chromosome 13 at 93,807,616 bp
  • T to C, chromosome 13 at 106,842,182 bp
  • C to T, chromosome 13 at 114,970,491 bp
  • T to C, chromosome 14 at 65,392,079 bp
  • C to T, chromosome 14 at 77,035,173 bp
  • A to T, chromosome 15 at 5,002,749 bp
  • G to T, chromosome 15 at 76,183,110 bp
  • A to T, chromosome 15 at 95,241,543 bp
  • A to T, chromosome 15 at 98,604,259 bp
  • T to C, chromosome 16 at 23,974,954 bp
  • A to G, chromosome 17 at 25,919,433 bp
  • T to A, chromosome 18 at 37,506,093 bp
  • T to C, chromosome 18 at 60,697,124 bp
  • C to T, chromosome 19 at 3,268,726 bp
  • G to C, chromosome 19 at 3,362,211 bp
  • A to T, chromosome 19 at 12,088,692 bp
  • T to A, chromosome 19 at 13,027,463 bp
  • A to C, chromosome 19 at 40,738,875 bp

Strain Description

Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Strain Origin

Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8951 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain

Reproductive Statistics

Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
068788-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.