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Strain Name:
C57BL/6J-MtgxR8962Btlr/Mmmh
Stock Number:
068796-MU
Citation ID:
RRID:MMRRC_068796-MU
Other Names:
R8962 (G1)
Major Collection:

Strain Information

S1pr1
Name: sphingosine-1-phosphate receptor 1
Synonyms: S1P1, S1P, Edg1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 13609
HGNC: HGNC:3165
Homologene: 1071
Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Trmt9b
Name: tRNA methyltransferase 9B
Synonyms: 6430573F11Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 319582
Homologene: 35306
Nos1
Name: nitric oxide synthase 1, neuronal
Synonyms: nNOS, bNOS, Nos-1, NO, 2310005C01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 18125
HGNC: HGNC:7872
Homologene: 37327
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Tkfc
Name: triokinase, FMN cyclase
Synonyms: Dak
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225913
VEGA: 19
Homologene: 56710
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 63,308,003 bp
  • T to C, chromosome 2 at 12,191,234 bp
  • A to T, chromosome 2 at 77,212,364 bp
  • A to T, chromosome 2 at 120,344,206 bp
  • G to C, chromosome 2 at 144,305,914 bp
  • C to T, chromosome 2 at 163,825,240 bp
  • C to A, chromosome 2 at 169,884,604 bp
  • T to C, chromosome 3 at 64,491,143 bp
  • A to T, chromosome 3 at 64,556,155 bp
  • T to C, chromosome 3 at 115,711,920 bp
  • T to C, chromosome 3 at 133,488,043 bp
  • A to G, chromosome 4 at 126,347,802 bp
  • T to C, chromosome 4 at 156,350,891 bp
  • A to T, chromosome 5 at 4,196,437 bp
  • T to G, chromosome 5 at 26,085,747 bp
  • T to C, chromosome 5 at 33,259,272 bp
  • T to A, chromosome 5 at 112,858,480 bp
  • T to C, chromosome 5 at 117,879,340 bp
  • T to C, chromosome 6 at 91,777,410 bp
  • T to C, chromosome 6 at 118,535,143 bp
  • T to A, chromosome 7 at 5,675,602 bp
  • T to A, chromosome 7 at 24,606,257 bp
  • A to G, chromosome 7 at 27,879,361 bp
  • T to G, chromosome 7 at 43,351,716 bp
  • T to C, chromosome 7 at 44,552,039 bp
  • T to G, chromosome 7 at 67,075,309 bp
  • T to A, chromosome 7 at 86,562,109 bp
  • G to A, chromosome 7 at 118,561,005 bp
  • T to A, chromosome 7 at 123,401,446 bp
  • A to G, chromosome 8 at 11,537,304 bp
  • A to G, chromosome 8 at 36,505,575 bp
  • T to A, chromosome 8 at 78,315,707 bp
  • A to C, chromosome 9 at 45,898,430 bp
  • T to A, chromosome 9 at 46,308,875 bp
  • A to G, chromosome 9 at 53,238,077 bp
  • A to G, chromosome 9 at 79,631,619 bp
  • C to T, chromosome 9 at 108,294,509 bp
  • A to T, chromosome 9 at 120,094,547 bp
  • T to G, chromosome 10 at 24,000,461 bp
  • T to C, chromosome 10 at 24,820,615 bp
  • A to T, chromosome 10 at 43,946,419 bp
  • G to T, chromosome 10 at 80,520,062 bp
  • A to G, chromosome 10 at 91,067,204 bp
  • A to T, chromosome 10 at 128,520,697 bp
  • A to T, chromosome 11 at 76,461,329 bp
  • G to A, chromosome 11 at 86,548,833 bp
  • G to T, chromosome 11 at 105,389,989 bp
  • A to T, chromosome 11 at 110,078,808 bp
  • T to C, chromosome 12 at 38,139,495 bp
  • T to C, chromosome 12 at 103,689,872 bp
  • A to T, chromosome 12 at 114,366,584 bp
  • T to A, chromosome 12 at 117,952,538 bp
  • C to T, chromosome 12 at 117,954,895 bp
  • G to A, chromosome 13 at 62,875,253 bp
  • G to T, chromosome 13 at 98,287,313 bp
  • A to T, chromosome 13 at 104,297,391 bp
  • G to A, chromosome 14 at 30,953,958 bp
  • A to G, chromosome 14 at 32,835,379 bp
  • A to G, chromosome 14 at 44,520,692 bp
  • A to G, chromosome 14 at 47,663,791 bp
  • A to C, chromosome 14 at 50,281,359 bp
  • T to C, chromosome 14 at 67,757,191 bp
  • C to T, chromosome 15 at 44,536,895 bp
  • C to A, chromosome 15 at 74,743,400 bp
  • G to T, chromosome 15 at 79,717,728 bp
  • C to A, chromosome 15 at 97,019,803 bp
  • A to G, chromosome 15 at 102,218,602 bp
  • C to A, chromosome 15 at 102,366,445 bp
  • A to G, chromosome 16 at 8,578,302 bp
  • A to G, chromosome 16 at 10,784,778 bp
  • T to C, chromosome 16 at 17,506,336 bp
  • A to G, chromosome 16 at 35,856,817 bp
  • T to C, chromosome 16 at 36,913,616 bp
  • C to T, chromosome 16 at 43,821,479 bp
  • T to C, chromosome 16 at 91,658,169 bp
  • T to A, chromosome 17 at 26,012,033 bp
  • A to T, chromosome 17 at 32,798,648 bp
  • A to G, chromosome 18 at 20,409,259 bp
  • T to C, chromosome 18 at 34,768,176 bp
  • T to A, chromosome 18 at 37,715,826 bp
  • A to G, chromosome 18 at 84,558,932 bp
  • T to C, chromosome 19 at 5,111,836 bp
  • T to C, chromosome 19 at 10,593,336 bp
  • T to C, chromosome 19 at 28,909,469 bp
  • C to T, chromosome 19 at 47,622,309 bp
  • T to C, chromosome 19 at 55,298,807 bp
  • C to T, chromosome 19 at 56,473,248 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8962 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068796-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.