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Strain Name:
C57BL/6J-MtgxR8990Btlr/Mmmh
Stock Number:
068822-MU
Citation ID:
RRID:MMRRC_068822-MU
Other Names:
R8990 (G1)
Major Collection:

Strain Information

Ntrk2
Name: neurotrophic tyrosine kinase, receptor, type 2
Synonyms: trkB, C030027L06Rik, Tkrb
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18212
VEGA: 13
HGNC: HGNC:8032
Homologene: 4504
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Kcnc1
Name: potassium voltage gated channel, Shaw-related subfamily, member 1
Synonyms: Kv3.1, KV4, NGK2, KShIIIB, Kcr2-1, Shaw, C230009H10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16502
HGNC: HGNC:6233
Homologene: 68134
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22064
Homologene: 135989
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Csnk2a1
Name: casein kinase 2, alpha 1 polypeptide
Synonyms: Csnk2a1-rs4, CK2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12995
Homologene: 90874
Hdac11
Name: histone deacetylase 11
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232232
Homologene: 11743
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 20,347,305 bp
  • C to T, chromosome 1 at 53,346,742 bp
  • C to T, chromosome 1 at 78,498,849 bp
  • A to G, chromosome 1 at 82,495,834 bp
  • A to T, chromosome 1 at 107,522,698 bp
  • C to T, chromosome 1 at 128,179,096 bp
  • A to G, chromosome 1 at 164,328,333 bp
  • T to C, chromosome 2 at 53,126,959 bp
  • T to G, chromosome 2 at 60,358,559 bp
  • C to T, chromosome 2 at 69,274,150 bp
  • G to T, chromosome 2 at 120,585,035 bp
  • A to G, chromosome 2 at 130,422,551 bp
  • A to G, chromosome 2 at 152,254,257 bp
  • T to C, chromosome 3 at 36,921,221 bp
  • A to G, chromosome 3 at 127,048,180 bp
  • A to G, chromosome 4 at 88,721,968 bp
  • T to C, chromosome 4 at 118,727,027 bp
  • T to C, chromosome 4 at 142,141,636 bp
  • G to A, chromosome 5 at 15,653,600 bp
  • T to C, chromosome 5 at 31,190,627 bp
  • A to G, chromosome 5 at 57,722,022 bp
  • A to G, chromosome 5 at 111,418,515 bp
  • A to G, chromosome 5 at 124,736,993 bp
  • T to G, chromosome 5 at 139,170,196 bp
  • A to G, chromosome 5 at 145,165,237 bp
  • T to A, chromosome 6 at 91,168,817 bp
  • A to G, chromosome 6 at 134,423,667 bp
  • T to A, chromosome 7 at 3,821,274 bp
  • T to A, chromosome 7 at 22,871,334 bp
  • G to T, chromosome 7 at 23,754,531 bp
  • T to A, chromosome 7 at 43,826,830 bp
  • T to A, chromosome 7 at 46,428,309 bp
  • T to C, chromosome 7 at 102,095,988 bp
  • T to C, chromosome 7 at 145,322,178 bp
  • C to T, chromosome 8 at 61,515,245 bp
  • C to T, chromosome 8 at 61,974,134 bp
  • G to C, chromosome 8 at 71,476,557 bp
  • G to A, chromosome 9 at 51,147,502 bp
  • A to G, chromosome 10 at 43,525,237 bp
  • T to A, chromosome 10 at 77,706,824 bp
  • C to A, chromosome 10 at 93,478,453 bp
  • A to T, chromosome 11 at 59,548,758 bp
  • A to T, chromosome 11 at 102,405,069 bp
  • A to G, chromosome 11 at 120,711,765 bp
  • G to T, chromosome 11 at 121,069,875 bp
  • G to A, chromosome 12 at 17,175,335 bp
  • A to G, chromosome 12 at 17,291,646 bp
  • T to C, chromosome 12 at 84,171,606 bp
  • T to A, chromosome 12 at 118,849,387 bp
  • A to G, chromosome 13 at 49,688,276 bp
  • T to C, chromosome 13 at 56,804,672 bp
  • A to T, chromosome 13 at 57,696,171 bp
  • T to A, chromosome 13 at 58,860,174 bp
  • G to A, chromosome 13 at 63,156,614 bp
  • T to C, chromosome 14 at 27,486,698 bp
  • C to A, chromosome 16 at 32,752,209 bp
  • A to T, chromosome 17 at 5,417,745 bp
  • T to C, chromosome 17 at 31,615,549 bp
  • C to A, chromosome 18 at 13,846,080 bp
  • A to T, chromosome 18 at 37,855,804 bp
  • G to A, chromosome 19 at 18,815,435 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R8990 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068822-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.