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Strain Name:
C57BL/6J-MtgxR9226Btlr/Mmmh
Stock Number:
068960-MU
Citation ID:
RRID:MMRRC_068960-MU
Other Names:
R9226 (G1)
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Pygb
Name: brain glycogen phosphorylase
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 110078
HGNC: HGNC:9723
Homologene: 100930
Strn4
Name: striatin, calmodulin binding protein 4
Synonyms: ZIN, zinedin
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 97387
Homologene: 8378
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Ankrd12
Name: ankyrin repeat domain 12
Synonyms: ANCO-2, GAC-1, 2900001A12Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 106585
Homologene: 9059
Sfmbt2
Name: Scm-like with four mbt domains 2
Synonyms: D330030P06Rik, D2Wsu23e
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 353282
Homologene: 82485
Pkn2
Name: protein kinase N2
Synonyms: PRK2, 6030436C20Rik, Stk7, Prkcl2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109333
HGNC: HGNC:9406
Homologene: 2054
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 44,086,973 bp
  • A to C, chromosome 1 at 53,521,167 bp
  • G to T, chromosome 1 at 74,395,576 bp
  • C to T, chromosome 1 at 167,308,563 bp
  • T to A, chromosome 2 at 6,047,034 bp
  • T to C, chromosome 2 at 10,438,049 bp
  • C to A, chromosome 2 at 31,017,400 bp
  • T to C, chromosome 2 at 41,511,448 bp
  • T to C, chromosome 2 at 140,255,302 bp
  • C to A, chromosome 2 at 150,820,861 bp
  • T to C, chromosome 2 at 170,496,357 bp
  • G to A, chromosome 3 at 142,793,948 bp
  • G to T, chromosome 3 at 151,492,427 bp
  • A to G, chromosome 4 at 32,694,612 bp
  • A to T, chromosome 5 at 87,970,659 bp
  • G to A, chromosome 5 at 112,378,039 bp
  • G to A, chromosome 5 at 115,480,313 bp
  • T to A, chromosome 6 at 33,918,424 bp
  • A to T, chromosome 6 at 115,957,563 bp
  • A to T, chromosome 6 at 136,854,367 bp
  • C to T, chromosome 7 at 5,053,138 bp
  • G to A, chromosome 7 at 13,029,563 bp
  • T to C, chromosome 7 at 13,325,746 bp
  • T to C, chromosome 7 at 16,825,797 bp
  • T to C, chromosome 7 at 28,757,136 bp
  • A to G, chromosome 7 at 44,462,675 bp
  • A to T, chromosome 7 at 81,593,907 bp
  • A to G, chromosome 7 at 97,378,216 bp
  • A to G, chromosome 7 at 103,358,885 bp
  • A to G, chromosome 7 at 116,083,771 bp
  • A to G, chromosome 8 at 13,310,143 bp
  • T to C, chromosome 8 at 83,929,797 bp
  • T to C, chromosome 8 at 107,368,769 bp
  • A to G, chromosome 9 at 31,064,228 bp
  • A to G, chromosome 9 at 40,074,891 bp
  • C to A, chromosome 9 at 52,081,168 bp
  • A to G, chromosome 9 at 104,008,129 bp
  • T to C, chromosome 10 at 81,119,359 bp
  • A to C, chromosome 10 at 81,274,586 bp
  • A to T, chromosome 11 at 20,829,306 bp
  • T to A, chromosome 11 at 54,994,965 bp
  • C to T, chromosome 11 at 74,858,134 bp
  • C to A, chromosome 11 at 115,792,365 bp
  • A to G, chromosome 11 at 115,860,098 bp
  • A to G, chromosome 12 at 33,082,321 bp
  • A to T, chromosome 12 at 115,055,453 bp
  • T to G, chromosome 14 at 31,145,855 bp
  • T to A, chromosome 14 at 60,831,243 bp
  • T to C, chromosome 14 at 65,931,784 bp
  • T to C, chromosome 15 at 82,620,700 bp
  • C to A, chromosome 16 at 37,255,844 bp
  • T to G, chromosome 17 at 20,471,068 bp
  • T to C, chromosome 17 at 25,098,865 bp
  • T to A, chromosome 17 at 34,685,792 bp
  • C to A, chromosome 17 at 42,669,715 bp
  • T to A, chromosome 17 at 65,985,759 bp
  • A to G, chromosome 19 at 53,851,214 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9226 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068960-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.