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Strain Name:
C57BL/6J-MtgxR9178Btlr/Mmmh
Stock Number:
068978-MU
Citation ID:
RRID:MMRRC_068978-MU
Other Names:
R9178 (G1)
Major Collection:

Strain Information

Zic4
Name: zinc finger protein of the cerebellum 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22774
Homologene: 32075
Ptf1a
Name: pancreas specific transcription factor, 1a
Synonyms: PTF1-p48, bHLHa29
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 19213
Homologene: 10304
Nrcam
Name: neuronal cell adhesion molecule
Synonyms: Bravo, C030017F07Rik, C130076O07Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319504
VEGA: 12
HGNC: HGNC:7994
Homologene: 21041
Icos
Name: inducible T cell co-stimulator
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 54167
HGNC: HGNC:5351
Homologene: 8097
Adcy10
Name: adenylate cyclase 10
Synonyms: soluble adenylyl cyclase, sAC, 4930431D04Rik, Sacy
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 271639
Homologene: 10188
Ints6
Name: integrator complex subunit 6
Synonyms: Notch2l, 2900075H24Rik, DICE1, Ddx26
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18130
VEGA: 14
Homologene: 8121
Dennd2b
Name: DENN domain containing 2B
Synonyms: 2610305K15Rik, 2010004M01Rik, St5, Denn2b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 76954
Homologene: 3951
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 54,272,485 bp
  • G to T, chromosome 1 at 60,995,555 bp
  • A to T, chromosome 1 at 165,575,649 bp
  • T to C, chromosome 1 at 173,637,403 bp
  • C to T, chromosome 1 at 174,391,663 bp
  • T to C, chromosome 1 at 180,130,836 bp
  • T to A, chromosome 2 at 18,045,678 bp
  • C to T, chromosome 2 at 19,445,725 bp
  • T to C, chromosome 2 at 31,391,509 bp
  • A to T, chromosome 2 at 37,405,252 bp
  • C to A, chromosome 2 at 71,900,058 bp
  • G to A, chromosome 2 at 85,414,504 bp
  • A to T, chromosome 2 at 168,269,185 bp
  • A to G, chromosome 3 at 59,326,059 bp
  • G to T, chromosome 3 at 109,156,952 bp
  • T to C, chromosome 4 at 45,285,367 bp
  • T to C, chromosome 4 at 63,394,609 bp
  • T to G, chromosome 4 at 135,779,399 bp
  • G to A, chromosome 5 at 21,217,473 bp
  • A to G, chromosome 5 at 29,206,213 bp
  • A to G, chromosome 5 at 87,420,537 bp
  • T to G, chromosome 5 at 139,152,897 bp
  • T to A, chromosome 5 at 149,204,338 bp
  • T to C, chromosome 6 at 90,467,109 bp
  • A to G, chromosome 6 at 126,849,087 bp
  • G to A, chromosome 6 at 149,033,346 bp
  • T to A, chromosome 7 at 4,927,059 bp
  • T to G, chromosome 7 at 5,053,153 bp
  • T to C, chromosome 7 at 6,188,158 bp
  • A to G, chromosome 7 at 27,877,957 bp
  • C to A, chromosome 7 at 78,790,696 bp
  • T to C, chromosome 7 at 104,106,513 bp
  • T to A, chromosome 7 at 105,751,639 bp
  • C to T, chromosome 7 at 109,557,084 bp
  • C to T, chromosome 7 at 127,786,418 bp
  • T to A, chromosome 7 at 140,837,674 bp
  • T to C, chromosome 8 at 79,091,935 bp
  • A to G, chromosome 8 at 104,423,623 bp
  • T to A, chromosome 8 at 123,248,835 bp
  • C to A, chromosome 8 at 125,670,910 bp
  • A to G, chromosome 9 at 3,338,448 bp
  • T to A, chromosome 9 at 18,330,856 bp
  • T to A, chromosome 9 at 55,973,708 bp
  • T to A, chromosome 9 at 86,489,743 bp
  • A to T, chromosome 9 at 91,378,860 bp
  • A to G, chromosome 9 at 105,781,970 bp
  • A to T, chromosome 9 at 111,367,200 bp
  • G to A, chromosome 10 at 80,314,401 bp
  • G to T, chromosome 10 at 92,994,717 bp
  • A to G, chromosome 11 at 44,992,449 bp
  • A to G, chromosome 11 at 45,004,721 bp
  • G to A, chromosome 11 at 53,883,721 bp
  • C to A, chromosome 11 at 58,529,647 bp
  • T to A, chromosome 11 at 76,914,052 bp
  • A to T, chromosome 11 at 109,950,111 bp
  • A to T, chromosome 12 at 28,594,855 bp
  • G to T, chromosome 12 at 44,568,546 bp
  • A to T, chromosome 12 at 69,976,016 bp
  • A to G, chromosome 12 at 88,012,016 bp
  • T to C, chromosome 12 at 91,830,752 bp
  • G to A, chromosome 12 at 100,205,720 bp
  • G to T, chromosome 12 at 104,472,861 bp
  • C to T, chromosome 13 at 54,737,788 bp
  • T to C, chromosome 13 at 81,694,416 bp
  • T to C, chromosome 13 at 112,541,246 bp
  • G to T, chromosome 14 at 53,560,062 bp
  • A to T, chromosome 14 at 62,709,587 bp
  • G to T, chromosome 14 at 70,490,173 bp
  • A to T, chromosome 14 at 101,807,470 bp
  • T to A, chromosome 15 at 98,129,280 bp
  • A to T, chromosome 16 at 91,634,016 bp
  • C to T, chromosome 17 at 25,471,927 bp
  • A to G, chromosome 17 at 88,668,724 bp
  • G to A, chromosome 18 at 24,600,913 bp
  • T to A, chromosome 18 at 37,727,839 bp
  • A to T, chromosome 18 at 61,310,922 bp
  • G to A, chromosome 19 at 46,652,494 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9178 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068978-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.