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Strain Name:
C57BL/6J-MtgxR9208Btlr/Mmmh
Stock Number:
068982-MU
Citation ID:
RRID:MMRRC_068982-MU
Other Names:
R9208 (G1)
Major Collection:

Strain Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Atl3
Name: atlastin GTPase 3
Synonyms: 4633402C03Rik, 5730596K20Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 109168
VEGA: 19
Homologene: 9149
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Pam, C130061D10Rik, Phr1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 105689
Homologene: 9005
Trmt13
Name: tRNA methyltransferase 13
Synonyms: A930028L21Rik, 4631408H19Rik, Ccdc76
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229780
Homologene: 6875
Patj
Name: PATJ, crumbs cell polarity complex component
Synonyms: Cipp, Inadl
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12695
Homologene: 72199
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Dctn1
Name: dynactin 1
Synonyms: Glued, p150, p150glued
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 13191
HGNC: HGNC:2711
Homologene: 3011
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 12,989,578 bp
  • T to C, chromosome 1 at 44,178,343 bp
  • T to C, chromosome 1 at 66,934,524 bp
  • A to G, chromosome 1 at 106,876,123 bp
  • A to G, chromosome 2 at 57,109,081 bp
  • C to A, chromosome 2 at 89,943,381 bp
  • T to C, chromosome 2 at 126,594,680 bp
  • T to A, chromosome 2 at 128,895,423 bp
  • T to A, chromosome 2 at 130,023,698 bp
  • T to A, chromosome 2 at 158,768,546 bp
  • C to A, chromosome 2 at 166,941,265 bp
  • T to A, chromosome 3 at 31,227,210 bp
  • T to A, chromosome 3 at 88,652,083 bp
  • T to C, chromosome 3 at 89,235,159 bp
  • T to A, chromosome 3 at 116,582,707 bp
  • A to T, chromosome 4 at 58,875,444 bp
  • A to G, chromosome 4 at 63,834,213 bp
  • A to T, chromosome 4 at 98,539,073 bp
  • A to G, chromosome 4 at 123,684,132 bp
  • A to T, chromosome 4 at 126,750,248 bp
  • A to C, chromosome 4 at 156,222,402 bp
  • T to A, chromosome 5 at 3,850,995 bp
  • T to C, chromosome 5 at 33,414,075 bp
  • T to A, chromosome 5 at 41,761,771 bp
  • A to G, chromosome 5 at 76,237,024 bp
  • T to C, chromosome 5 at 124,312,791 bp
  • A to T, chromosome 6 at 13,082,431 bp
  • A to T, chromosome 6 at 32,517,444 bp
  • T to C, chromosome 6 at 83,199,702 bp
  • C to T, chromosome 6 at 83,551,972 bp
  • A to G, chromosome 6 at 86,446,245 bp
  • A to G, chromosome 6 at 126,966,969 bp
  • T to C, chromosome 6 at 149,101,200 bp
  • C to T, chromosome 6 at 149,326,529 bp
  • A to T, chromosome 7 at 8,483,299 bp
  • T to C, chromosome 7 at 25,288,077 bp
  • A to G, chromosome 7 at 103,768,271 bp
  • A to G, chromosome 7 at 105,559,520 bp
  • T to A, chromosome 8 at 128,746,001 bp
  • T to A, chromosome 9 at 18,508,537 bp
  • T to C, chromosome 9 at 38,189,824 bp
  • A to G, chromosome 9 at 57,682,300 bp
  • T to A, chromosome 9 at 62,849,183 bp
  • T to A, chromosome 9 at 88,383,779 bp
  • C to A, chromosome 9 at 110,408,033 bp
  • A to G, chromosome 10 at 34,297,572 bp
  • A to G, chromosome 10 at 70,553,039 bp
  • A to G, chromosome 10 at 81,509,390 bp
  • T to C, chromosome 11 at 69,104,810 bp
  • A to G, chromosome 12 at 34,170,102 bp
  • T to A, chromosome 12 at 84,106,584 bp
  • C to T, chromosome 12 at 118,027,516 bp
  • A to T, chromosome 13 at 22,187,231 bp
  • T to A, chromosome 14 at 6,741,416 bp
  • C to T, chromosome 14 at 73,177,060 bp
  • T to C, chromosome 14 at 103,295,228 bp
  • C to A, chromosome 15 at 98,437,733 bp
  • T to C, chromosome 16 at 76,292,728 bp
  • T to C, chromosome 16 at 87,400,410 bp
  • A to G, chromosome 16 at 88,671,428 bp
  • A to T, chromosome 17 at 17,970,869 bp
  • A to G, chromosome 17 at 23,175,577 bp
  • C to T, chromosome 17 at 25,742,720 bp
  • A to G, chromosome 17 at 33,640,827 bp
  • A to T, chromosome 17 at 48,307,894 bp
  • T to C, chromosome 18 at 7,403,327 bp
  • A to G, chromosome 19 at 7,510,082 bp
  • T to A, chromosome 19 at 40,365,018 bp
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9208 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
068982-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.