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Strain Name:
C57BL/6J-MtgxR9154Btlr/Mmmh
Stock Number:
069018-MU
Citation ID:
RRID:MMRRC_069018-MU
Other Names:
R9154 (G1)
Major Collection:

Strain Information

Chrna4
Name: cholinergic receptor, nicotinic, alpha polypeptide 4
Synonyms: alpha4 nAChR, Acra-4, Acra4, a4 nicotinic receptor, alpha4-nAChR
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11438
HGNC: HGNC:1958
Homologene: 592
Nsd1
Name: nuclear receptor-binding SET-domain protein 1
Synonyms: KMT3B
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 18193
VEGA: 13
Homologene: 32543
Fiz1
Name: Flt3 interacting zinc finger protein 1
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 23877
Homologene: 22663
Tanc1
Name: tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1
Synonyms: 1200003E16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66860
Homologene: 18946
Luc7l3
Name: LUC7-like 3 (S. cerevisiae)
Synonyms: 3300001P08Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67684
Homologene: 75056
Fus
Name: fused in sarcoma
Synonyms: translocated in liposarcoma, Tls, hnRNP P2, pigpen, D930039C12Rik, D430004D17Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 233908
HGNC: HGNC:4010
Homologene: 134091
Nfx1
Name: nuclear transcription factor, X-box binding 1
Synonyms: TEG-42, 1300017N15Rik, 3000003M19Rik, Tex42
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74164
HGNC: HGNC:7803
Homologene: 1875
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to G, chromosome 1 at 83,257,261 bp (GRCm38)
  • G to T, chromosome 1 at 90,927,994 bp (GRCm38)
  • T to A, chromosome 1 at 93,501,588 bp (GRCm38)
  • A to G, chromosome 1 at 138,088,564 bp (GRCm38)
  • T to A, chromosome 1 at 163,062,461 bp (GRCm38)
  • A to G, chromosome 1 at 171,461,808 bp (GRCm38)
  • T to A, chromosome 2 at 23,187,803 bp (GRCm38)
  • T to C, chromosome 2 at 59,799,788 bp (GRCm38)
  • T to C, chromosome 2 at 91,167,817 bp (GRCm38)
  • G to A, chromosome 2 at 120,534,331 bp (GRCm38)
  • G to A, chromosome 2 at 125,775,703 bp (GRCm38)
  • A to G, chromosome 2 at 165,280,877 bp (GRCm38)
  • C to A, chromosome 2 at 168,184,660 bp (GRCm38)
  • G to A, chromosome 2 at 181,028,809 bp (GRCm38)
  • A to G, chromosome 3 at 28,650,086 bp (GRCm38)
  • G to A, chromosome 3 at 32,343,618 bp (GRCm38)
  • A to T, chromosome 3 at 89,958,620 bp (GRCm38)
  • A to T, chromosome 3 at 133,132,163 bp (GRCm38)
  • A to T, chromosome 3 at 146,593,571 bp (GRCm38)
  • A to G, chromosome 3 at 154,247,736 bp (GRCm38)
  • T to A, chromosome 4 at 40,990,845 bp (GRCm38)
  • T to C, chromosome 4 at 43,372,630 bp (GRCm38)
  • T to C, chromosome 4 at 45,106,724 bp (GRCm38)
  • G to A, chromosome 4 at 134,065,309 bp (GRCm38)
  • T to A, chromosome 5 at 17,376,612 bp (GRCm38)
  • T to C, chromosome 5 at 74,587,042 bp (GRCm38)
  • G to A, chromosome 5 at 121,253,904 bp (GRCm38)
  • C to A, chromosome 5 at 122,551,059 bp (GRCm38)
  • T to C, chromosome 5 at 137,921,496 bp (GRCm38)
  • A to T, chromosome 6 at 5,265,391 bp (GRCm38)
  • T to C, chromosome 6 at 42,435,240 bp (GRCm38)
  • C to A, chromosome 6 at 57,844,363 bp (GRCm38)
  • T to A, chromosome 6 at 71,510,115 bp (GRCm38)
  • T to C, chromosome 6 at 91,949,945 bp (GRCm38)
  • A to T, chromosome 6 at 121,668,553 bp (GRCm38)
  • A to T, chromosome 6 at 121,779,477 bp (GRCm38)
  • T to C, chromosome 6 at 130,380,644 bp (GRCm38)
  • A to G, chromosome 6 at 134,541,892 bp (GRCm38)
  • G to A, chromosome 7 at 5,008,281 bp (GRCm38)
  • A to C, chromosome 7 at 7,135,303 bp (GRCm38)
  • T to A, chromosome 7 at 10,105,553 bp (GRCm38)
  • C to T, chromosome 7 at 14,073,056 bp (GRCm38)
  • C to A, chromosome 7 at 25,058,978 bp (GRCm38)
  • T to C, chromosome 7 at 29,069,858 bp (GRCm38)
  • C to T, chromosome 7 at 42,926,989 bp (GRCm38)
  • T to G, chromosome 7 at 47,426,646 bp (GRCm38)
  • A to G, chromosome 7 at 80,247,434 bp (GRCm38)
  • T to C, chromosome 7 at 102,709,334 bp (GRCm38)
  • G to A, chromosome 7 at 125,455,124 bp (GRCm38)
  • G to A, chromosome 7 at 127,981,268 bp (GRCm38)
  • A to C, chromosome 7 at 141,864,237 bp (GRCm38)
  • T to A, chromosome 8 at 11,217,446 bp (GRCm38)
  • A to G, chromosome 8 at 23,115,371 bp (GRCm38)
  • CCAGCAGCAGCAGCAGCAG to CCAGCAGCAGCAGCAG, chromosome 8 at 24,948,753 bp (GRCm38)
  • C to T, chromosome 8 at 104,628,711 bp (GRCm38)
  • G to T, chromosome 8 at 109,939,958 bp (GRCm38)
  • A to C, chromosome 8 at 119,679,377 bp (GRCm38)
  • T to A, chromosome 9 at 19,441,235 bp (GRCm38)
  • A to G, chromosome 9 at 20,212,877 bp (GRCm38)
  • A to T, chromosome 9 at 21,230,825 bp (GRCm38)
  • T to A, chromosome 9 at 38,026,394 bp (GRCm38)
  • T to C, chromosome 9 at 43,222,695 bp (GRCm38)
  • T to A, chromosome 9 at 56,891,003 bp (GRCm38)
  • T to C, chromosome 10 at 25,171,155 bp (GRCm38)
  • A to G, chromosome 10 at 51,721,504 bp (GRCm38)
  • T to G, chromosome 10 at 52,046,205 bp (GRCm38)
  • T to C, chromosome 10 at 129,472,821 bp (GRCm38)
  • A to T, chromosome 11 at 4,794,873 bp (GRCm38)
  • A to G, chromosome 11 at 67,362,492 bp (GRCm38)
  • A to G, chromosome 11 at 86,234,698 bp (GRCm38)
  • A to T, chromosome 11 at 94,299,967 bp (GRCm38)
  • C to A, chromosome 11 at 99,798,441 bp (GRCm38)
  • T to C, chromosome 11 at 102,020,502 bp (GRCm38)
  • G to T, chromosome 12 at 84,101,015 bp (GRCm38)
  • A to G, chromosome 13 at 23,739,390 bp (GRCm38)
  • C to T, chromosome 13 at 55,213,440 bp (GRCm38)
  • A to T, chromosome 14 at 49,235,910 bp (GRCm38)
  • T to A, chromosome 14 at 101,885,307 bp (GRCm38)
  • A to G, chromosome 15 at 5,117,627 bp (GRCm38)
  • A to G, chromosome 15 at 89,011,915 bp (GRCm38)
  • A to T, chromosome 16 at 4,940,417 bp (GRCm38)
  • T to C, chromosome 16 at 35,948,173 bp (GRCm38)
  • A to G, chromosome 19 at 10,648,486 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9154 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069018-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.