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Strain Name:
C57BL/6J-MtgxR9225Btlr/Mmmh
Stock Number:
069059-MU
Citation ID:
RRID:MMRRC_069059-MU
Other Names:
R9225 (G1)
Major Collection:

Strain Information

Rpl13a
Name: ribosomal protein L13A
Synonyms: tum-antigen, tum-transplantation antigen P198, Tstap198-7, 1810026N22Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 22121
Homologene: 111053
Neurod1
Name: neurogenic differentiation 1
Synonyms: BETA2, Neurod, bHLHa3, Nd1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18012
HGNC: HGNC:7762
Homologene: 1871
Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Mycn
Name: v-myc avian myelocytomatosis viral related oncogene, neuroblastoma derived
Synonyms: Nmyc, N-myc, Nmyc-1, Nmyc1, bHLHe37
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18109
HGNC: HGNC:7559
Homologene: 3922
Slamf6
Name: SLAM family member 6
Synonyms: SF2000, NTB-A, KAL1b, KAL1, Ly108
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 30925
Homologene: 49945
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Spen
Name: spen family transcription repressor
Synonyms: Mint
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56381
Homologene: 124461
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 88,138,838 bp (GRCm38)
  • A to T, chromosome 1 at 92,856,020 bp (GRCm38)
  • T to C, chromosome 1 at 171,936,703 bp (GRCm38)
  • A to G, chromosome 1 at 173,690,728 bp (GRCm38)
  • A to G, chromosome 2 at 17,400,511 bp (GRCm38)
  • T to C, chromosome 2 at 34,370,926 bp (GRCm38)
  • A to G, chromosome 2 at 52,111,850 bp (GRCm38)
  • C to A, chromosome 2 at 73,273,495 bp (GRCm38)
  • A to T, chromosome 2 at 79,454,387 bp (GRCm38)
  • T to A, chromosome 2 at 83,880,042 bp (GRCm38)
  • T to C, chromosome 2 at 91,688,783 bp (GRCm38)
  • C to A, chromosome 2 at 110,012,140 bp (GRCm38)
  • T to A, chromosome 2 at 155,407,521 bp (GRCm38)
  • T to A, chromosome 2 at 157,265,090 bp (GRCm38)
  • A to C, chromosome 3 at 27,456,531 bp (GRCm38)
  • T to A, chromosome 3 at 35,964,362 bp (GRCm38)
  • G to T, chromosome 3 at 64,126,600 bp (GRCm38)
  • A to T, chromosome 3 at 69,552,911 bp (GRCm38)
  • A to G, chromosome 3 at 126,942,462 bp (GRCm38)
  • A to G, chromosome 4 at 18,907,010 bp (GRCm38)
  • A to T, chromosome 4 at 19,601,385 bp (GRCm38)
  • C to T, chromosome 4 at 49,530,193 bp (GRCm38)
  • T to C, chromosome 4 at 141,475,632 bp (GRCm38)
  • A to G, chromosome 5 at 35,976,624 bp (GRCm38)
  • G to T, chromosome 5 at 87,972,574 bp (GRCm38)
  • A to T, chromosome 5 at 91,957,537 bp (GRCm38)
  • T to C, chromosome 5 at 109,221,422 bp (GRCm38)
  • A to T, chromosome 5 at 145,713,604 bp (GRCm38)
  • G to T, chromosome 6 at 93,785,530 bp (GRCm38)
  • T to C, chromosome 6 at 120,218,967 bp (GRCm38)
  • A to T, chromosome 6 at 137,530,563 bp (GRCm38)
  • T to C, chromosome 7 at 23,417,946 bp (GRCm38)
  • T to C, chromosome 7 at 26,937,777 bp (GRCm38)
  • A to G, chromosome 7 at 30,586,747 bp (GRCm38)
  • A to G, chromosome 7 at 44,334,046 bp (GRCm38)
  • A to T, chromosome 7 at 44,960,789 bp (GRCm38)
  • C to A, chromosome 7 at 45,126,203 bp (GRCm38)
  • C to A, chromosome 7 at 45,126,204 bp (GRCm38)
  • A to G, chromosome 7 at 63,757,721 bp (GRCm38)
  • C to T, chromosome 7 at 80,863,273 bp (GRCm38)
  • T to A, chromosome 7 at 85,559,034 bp (GRCm38)
  • A to G, chromosome 7 at 101,301,981 bp (GRCm38)
  • A to G, chromosome 7 at 105,568,856 bp (GRCm38)
  • A to G, chromosome 7 at 106,677,769 bp (GRCm38)
  • A to G, chromosome 7 at 141,993,302 bp (GRCm38)
  • T to A, chromosome 8 at 4,234,561 bp (GRCm38)
  • G to A, chromosome 8 at 12,817,005 bp (GRCm38)
  • A to G, chromosome 8 at 25,771,242 bp (GRCm38)
  • G to T, chromosome 8 at 43,520,416 bp (GRCm38)
  • G to A, chromosome 8 at 62,017,841 bp (GRCm38)
  • T to C, chromosome 8 at 105,840,027 bp (GRCm38)
  • A to G, chromosome 8 at 109,858,877 bp (GRCm38)
  • G to A, chromosome 9 at 77,452,673 bp (GRCm38)
  • C to T, chromosome 9 at 105,782,238 bp (GRCm38)
  • G to A, chromosome 9 at 107,526,204 bp (GRCm38)
  • A to G, chromosome 9 at 120,464,784 bp (GRCm38)
  • A to T, chromosome 10 at 29,196,331 bp (GRCm38)
  • G to T, chromosome 10 at 30,547,757 bp (GRCm38)
  • C to A, chromosome 10 at 94,793,199 bp (GRCm38)
  • A to T, chromosome 10 at 122,449,561 bp (GRCm38)
  • A to G, chromosome 11 at 29,205,648 bp (GRCm38)
  • T to C, chromosome 11 at 60,710,063 bp (GRCm38)
  • A to T, chromosome 11 at 68,588,235 bp (GRCm38)
  • C to A, chromosome 11 at 73,353,769 bp (GRCm38)
  • T to C, chromosome 11 at 96,366,293 bp (GRCm38)
  • G to T, chromosome 12 at 8,771,817 bp (GRCm38)
  • C to A, chromosome 12 at 12,937,608 bp (GRCm38)
  • A to G, chromosome 12 at 26,327,607 bp (GRCm38)
  • G to A, chromosome 12 at 40,829,670 bp (GRCm38)
  • G to A, chromosome 13 at 23,136,654 bp (GRCm38)
  • T to A, chromosome 13 at 40,860,860 bp (GRCm38)
  • T to A, chromosome 13 at 42,183,708 bp (GRCm38)
  • T to A, chromosome 14 at 6,303,883 bp (GRCm38)
  • A to T, chromosome 14 at 77,034,974 bp (GRCm38)
  • T to C, chromosome 14 at 78,725,067 bp (GRCm38)
  • A to G, chromosome 14 at 87,007,324 bp (GRCm38)
  • A to G, chromosome 16 at 17,776,753 bp (GRCm38)
  • C to T, chromosome 17 at 25,122,722 bp (GRCm38)
  • A to C, chromosome 17 at 30,635,673 bp (GRCm38)
  • A to T, chromosome 17 at 32,925,345 bp (GRCm38)
  • T to C, chromosome 17 at 32,929,201 bp (GRCm38)
  • G to T, chromosome 17 at 84,441,744 bp (GRCm38)
  • A to G, chromosome 17 at 87,330,074 bp (GRCm38)
  • A to T, chromosome 18 at 37,308,642 bp (GRCm38)
  • T to C, chromosome 18 at 53,706,824 bp (GRCm38)
  • G to A, chromosome 19 at 5,950,464 bp (GRCm38)
  • A to T, chromosome 19 at 7,457,489 bp (GRCm38)
  • CGCAGCCGCACCTG to CG, chromosome 19 at 8,774,324 bp (GRCm38)
  • A to G, chromosome 19 at 13,017,006 bp (GRCm38)
  • G to C, chromosome 19 at 13,017,007 bp (GRCm38)
  • C to A, chromosome 19 at 40,180,468 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9225 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069059-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.