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Strain Name:
C57BL/6J-MtgxR9268Btlr/Mmmh
Stock Number:
069088-MU
Citation ID:
RRID:MMRRC_069088-MU
Other Names:
R9268 (G1)
Major Collection:

Strain Information

Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, Apoav, 1300007O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Rnf220
Name: ring finger protein 220
Synonyms: 5730503K05Rik, 4931406I20Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 66743
Homologene: 10036
Wdr47
Name: WD repeat domain 47
Synonyms: 1810073M12Rik, nemitin
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99512
Homologene: 8984
Sptlc1
Name: serine palmitoyltransferase, long chain base subunit 1
Synonyms: Lcb1, Spt1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 268656
VEGA: 13
Homologene: 4681
Acap2
Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Synonyms: 9530039J15Rik, Centb2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78618
VEGA: 16
Homologene: 8182
Etaa1
Name: Ewing tumor-associated antigen 1
Synonyms: 5730466H23Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68145
Homologene: 10369
Parp1
Name: poly (ADP-ribose) polymerase family, member 1
Synonyms: PARP, sPARP-1, Adprp, parp-1, 5830444G22Rik, Adprt1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 11545
HGNC: HGNC:270
Homologene: 1222
Papola
Name: poly (A) polymerase alpha
Synonyms: Plap, PapIII
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 18789
Homologene: 23389
Eif2ak4
Name: eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms: GCN2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27103
Homologene: 40891
Dtna
Name: dystrobrevin alpha
Synonyms: alpha-dystrobrevin, A0, 87K protein, Dtn, adbn, a-DB-1, 2210407P21Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 13527
VEGA: 18
HGNC: HGNC:3057
Homologene: 20362
Dctn4
Name: dynactin 4
Synonyms: p62, 4930547K17Rik, 1110001K06Rik, C130039E17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67665
VEGA: 18
Homologene: 9430
Adnp
Name: activity-dependent neuroprotective protein
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11538
Homologene: 7617
Tcf20
Name: transcription factor 20
Synonyms: SPBP, stromelysin 1 PDGF responsive element binding protein, 2810438H08Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 21411
VEGA: 15
Homologene: 4131
Egfr
Name: epidermal growth factor receptor
Synonyms: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, Erbb, Wa5, 9030024J15Rik, Errb1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 13649
HGNC: HGNC:3236
Homologene: 74545
Cdh19
Name: cadherin 19, type 2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 227485
HGNC: HGNC:1758
Homologene: 23286
Fuca2
Name: fucosidase, alpha-L- 2, plasma
Synonyms: 5530401P20Rik, 0610025O11Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 66848
HGNC: HGNC:4008
Homologene: 119
Clk1
Name: CDC-like kinase 1
Synonyms: STY, Clk1
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12747
HGNC: HGNC:2068
Homologene: 101535
Mfsd14b
Name: major facilitator superfamily domain containing 14B
Synonyms: 5730414C17Rik, Hiatl1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 66631
Homologene: 64464
Camk1d
Name: calcium/calmodulin-dependent protein kinase ID
Synonyms: CKLiK, E030025C11Rik, A630059D12Rik, CaMKIdelta
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227541
Homologene: 121858
Enam
Name: enamelin
Synonyms: abte
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 13801
HGNC: HGNC:3344
Homologene: 9698
Hjv
Name: hemojuvelin BMP co-receptor
Synonyms: 2310035L15Rik, 5230400G09Rik, DL-M, Rgmc, HJV, hemojuvelin, Hfe2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 69585
HGNC: HGNC:4887
Homologene: 17060
Rpgrip1l
Name: Rpgrip1-like
Synonyms: Ftm, 1700047E16Rik, fantom, Nphp8
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 244585
Homologene: 18296
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Nlgn1
Name: neuroligin 1
Synonyms: 6330415N05Rik, NL1, Nlg1
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 192167
Homologene: 56690
Lama4
Name: laminin, alpha 4
Synonyms: laminin [a]4
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 16775
HGNC: HGNC:6484
Homologene: 37604
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, HL-19, DLP12, HL19, DHC3, LOC380889, 4921531P07Rik, Dnahc7l, Dnahc12
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329628
Homologene: 14377
Cyp2j11
Name: cytochrome P450, family 2, subfamily j, polypeptide 11
Synonyms: Cyp2j11-ps
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100066
HGNC: HGNC:2634
Homologene: 133819
Wnk2
Name: WNK lysine deficient protein kinase 2
Synonyms: ESTM15, 1810073P09Rik, X83337
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 75607
Homologene: 19155
Or5h17
Name: olfactory receptor family 5 subfamily H member 17
Synonyms: GA_x54KRFPKG5P-55229158-55230087, MOR183-2, Olfr183
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 258478
Homologene: 27178
Peli2
Name: pellino 2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 93834
VEGA: 14
HGNC: HGNC:8828
Homologene: 41431
Clstn3
Name: calsyntenin 3
Synonyms: Cst-3, CSTN3, alcadein-beta, Clstn3b
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232370
Homologene: 22836
Slco3a1
Name: solute carrier organic anion transporter family, member 3a1
Synonyms: MJAM, OATP-D, 5830414C08Rik, Anr1, Slc21a11
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108116
Homologene: 40862
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: 4632428N09Rik, Aof1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218214
Homologene: 14695
Bace1
Name: beta-site APP cleaving enzyme 1
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 23821
HGNC: HGNC:933
Homologene: 8014
Myo3b
Name: myosin IIIB
Synonyms: A430065P19Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 329421
Homologene: 51393
F13a1
Name: coagulation factor XIII, A1 subunit
Synonyms: Factor XIIIA, 1200014I03Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 74145
VEGA: 13
HGNC: HGNC:3531
Homologene: 20077
Otogl
Name: otogelin-like
Synonyms: Gm6924
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 628870
Homologene: 46008
Atg2a
Name: autophagy related 2A
Synonyms: 1810013C15Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 329015
Homologene: 86985
Col15a1
Name: collagen, type XV, alpha 1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 12819
HGNC: HGNC:2192
Homologene: 1396
Zswim8
Name: zinc finger SWIM-type containing 8
Synonyms: 4832404P21Rik, 2310021P13Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 268721
VEGA: 14
Homologene: 34313
Sytl2
Name: synaptotagmin-like 2
Synonyms: Slp2-a, Slp2, Slp2-d, Slp2-c, Slp2-b
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 83671
Homologene: 131343
Klri2
Name: killer cell lectin-like receptor family I member 2
Synonyms: A530090P03Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320407
Homologene: 86736
Thumpd3
Name: THUMP domain containing 3
Synonyms: Gtrosa26as
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 14911
Homologene: 7351
Alk
Name: anaplastic lymphoma kinase
Synonyms: Tcrz, CD246
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 11682
VEGA: 17
HGNC: HGNC:427
Homologene: 68387
Asb15
Name: ankyrin repeat and SOCS box-containing 15
Synonyms: 4930400E23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 78910
Homologene: 43797
Or9i14
Name: olfactory receptor family 9 subfamily I member 14
Synonyms: GA_x6K02T2RE5P-4147744-4146800, MOR211-2, Olfr1499
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 258792
Homologene: 17393
Ssrp1
Name: structure specific recognition protein 1
Synonyms: T160, Hmgi-rs3, Hmg1-rs1, Hmgox
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20833
Homologene: 110735
Far2
Name: fatty acyl CoA reductase 2
Synonyms: Mlstd1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 330450
Homologene: 69247
Fhod3
Name: formin homology 2 domain containing 3
Synonyms: A930009H06Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225288
VEGA: 18
Homologene: 45323
Carf
Name: calcium response factor
Synonyms: Als2cr8
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 241066
Homologene: 11689
Ces1b
Name: carboxylesterase 1B
Synonyms: Gm5158
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382044
HGNC: HGNC:1863
Homologene: 117484
Nfatc4
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4
Synonyms: 3110041H08Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 73181
VEGA: 14
HGNC: HGNC:7778
Homologene: 3349
Hycc2
Name: hyccin PI4KA lipid kinase complex subunit 2
Synonyms: D1Ertd53e, Fam126b
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 213056
Homologene: 18184
Vmn1r29
Name: vomeronasal 1 receptor 29
Synonyms: V1rc2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113859
Homologene: 123821
Acadsb
Name: acyl-Coenzyme A dehydrogenase, short/branched chain
Synonyms: 1300003O09Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 66885
HGNC: HGNC:91
Homologene: 1216
Pus7l
Name: pseudouridylate synthase 7-like
Synonyms: 3000003F02Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 78895
Homologene: 12842
Shisa7
Name: shisa family member 7
Synonyms: D430041B17Rik, CKAMP59
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 232813
Homologene: 122129
Ift70a1
Name: intraflagellar transport 70A1
Synonyms: 4930506L13Rik, Ttc30a1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 78802
Homologene: 136638
Ulbp1
Name: UL16 binding protein 1
Synonyms: MULT1, A430108B07Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 77777
VEGA: 10
Homologene: 108274
Lrrc73
Name: leucine rich repeat containing 73
Synonyms: LOC224813, Gm88
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224813
VEGA: 17
Homologene: 19183
Rmi1
Name: RecQ mediated genome instability 1
Synonyms: 4932432N11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 74386
VEGA: 13
Homologene: 41601
Igkv4-72
Name: immunoglobulin kappa chain variable 4-72
Synonyms: LOC385109, Gm1499
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 385109
Msantd5f3
Name: Myb/SANT DNA binding domain containing 5 family member 3
Synonyms: Gm11238
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100502896
Homologene: 128423
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 58,419,723 bp (GRCm38)
  • T to C, chromosome 1 at 58,552,202 bp (GRCm38)
  • C to A, chromosome 1 at 60,109,399 bp (GRCm38)
  • A to G, chromosome 1 at 110,949,381 bp (GRCm38)
  • A to G, chromosome 1 at 180,587,944 bp (GRCm38)
  • A to G, chromosome 2 at 5,299,090 bp (GRCm38)
  • A to C, chromosome 2 at 70,426,961 bp (GRCm38)
  • A to T, chromosome 2 at 75,980,935 bp (GRCm38)
  • A to G, chromosome 2 at 76,937,735 bp (GRCm38)
  • C to A, chromosome 2 at 85,040,262 bp (GRCm38)
  • G to C, chromosome 2 at 118,441,220 bp (GRCm38)
  • A to G, chromosome 2 at 168,189,313 bp (GRCm38)
  • A to T, chromosome 3 at 25,436,384 bp (GRCm38)
  • C to T, chromosome 3 at 38,888,247 bp (GRCm38)
  • A to G, chromosome 3 at 90,061,723 bp (GRCm38)
  • T to C, chromosome 3 at 96,528,565 bp (GRCm38)
  • T to C, chromosome 3 at 108,618,496 bp (GRCm38)
  • G to C, chromosome 4 at 47,288,200 bp (GRCm38)
  • T to C, chromosome 4 at 73,657,136 bp (GRCm38)
  • A to T, chromosome 4 at 96,319,544 bp (GRCm38)
  • A to G, chromosome 4 at 117,489,636 bp (GRCm38)
  • T to C, chromosome 4 at 123,473,789 bp (GRCm38)
  • A to G, chromosome 5 at 88,492,919 bp (GRCm38)
  • G to A, chromosome 6 at 24,566,299 bp (GRCm38)
  • T to A, chromosome 6 at 58,307,592 bp (GRCm38)
  • A to T, chromosome 6 at 69,226,996 bp (GRCm38)
  • T to A, chromosome 6 at 113,066,858 bp (GRCm38)
  • G to A, chromosome 6 at 124,456,921 bp (GRCm38)
  • A to T, chromosome 6 at 129,733,074 bp (GRCm38)
  • C to T, chromosome 6 at 148,158,920 bp (GRCm38)
  • T to G, chromosome 7 at 4,834,334 bp (GRCm38)
  • T to C, chromosome 7 at 74,303,198 bp (GRCm38)
  • A to G, chromosome 7 at 90,385,151 bp (GRCm38)
  • C to T, chromosome 7 at 131,432,034 bp (GRCm38)
  • T to C, chromosome 8 at 91,280,727 bp (GRCm38)
  • A to C, chromosome 8 at 93,071,955 bp (GRCm38)
  • G to T, chromosome 9 at 45,855,984 bp (GRCm38)
  • A to T, chromosome 9 at 46,270,421 bp (GRCm38)
  • A to T, chromosome 10 at 7,446,392 bp (GRCm38)
  • G to A, chromosome 10 at 13,514,819 bp (GRCm38)
  • A to G, chromosome 10 at 39,074,692 bp (GRCm38)
  • A to T, chromosome 10 at 74,643,623 bp (GRCm38)
  • A to T, chromosome 10 at 107,781,056 bp (GRCm38)
  • T to A, chromosome 11 at 16,905,410 bp (GRCm38)
  • A to T, chromosome 11 at 17,946,419 bp (GRCm38)
  • A to G, chromosome 12 at 105,799,773 bp (GRCm38)
  • C to T, chromosome 13 at 36,892,936 bp (GRCm38)
  • A to C, chromosome 13 at 47,064,229 bp (GRCm38)
  • A to G, chromosome 13 at 49,082,031 bp (GRCm38)
  • A to T, chromosome 13 at 53,358,836 bp (GRCm38)
  • T to C, chromosome 13 at 58,408,039 bp (GRCm38)
  • A to G, chromosome 13 at 65,075,041 bp (GRCm38)
  • T to C, chromosome 14 at 20,711,840 bp (GRCm38)
  • A to G, chromosome 14 at 26,849,298 bp (GRCm38)
  • A to G, chromosome 14 at 48,281,470 bp (GRCm38)
  • A to T, chromosome 14 at 55,827,228 bp (GRCm38)
  • A to G, chromosome 15 at 82,856,504 bp (GRCm38)
  • T to C, chromosome 15 at 94,533,564 bp (GRCm38)
  • A to T, chromosome 16 at 31,136,574 bp (GRCm38)
  • T to G, chromosome 16 at 58,999,720 bp (GRCm38)
  • T to C, chromosome 17 at 46,254,609 bp (GRCm38)
  • T to C, chromosome 17 at 71,874,195 bp (GRCm38)
  • T to A, chromosome 18 at 23,569,586 bp (GRCm38)
  • T to C, chromosome 18 at 24,709,775 bp (GRCm38)
  • T to A, chromosome 18 at 60,526,232 bp (GRCm38)
  • A to C, chromosome 19 at 6,241,875 bp (GRCm38)
  • A to T, chromosome 19 at 13,815,024 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9268 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069088-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.