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Strain Name:
C57BL/6J-MtgxR9310Btlr/Mmmh
Stock Number:
069125-MU
Citation ID:
RRID:MMRRC_069125-MU
Other Names:
R9310 (G1)
Major Collection:

Strain Information

Cntnap2
Name: contactin associated protein-like 2
Synonyms: 5430425M22Rik, Caspr2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 66797
Homologene: 69159
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Mrtfb
Name: myocardin related transcription factor B
Synonyms: MRTF-B, Gt4-1, Mrtfb, Mkl2
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 239719
Homologene: 40917
Rfx3
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19726
HGNC: HGNC:9984
Homologene: 7917
Prkci
Name: protein kinase C, iota
Synonyms: Pkcl, Pkci, 2310021H13Rik, Prkcl, aPKClambda, PKClambda
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18759
HGNC: HGNC:9404
Homologene: 37667
Mtor
Name: mechanistic target of rapamycin kinase
Synonyms: FKBP-rapamycin-associated protein FRAP, 2610315D21Rik, RAPT1, RAFT1, flat, Frap1, mechanistic target of rapamycin (serine/threonine kinase)
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 56717
HGNC: HGNC:3942
Homologene: 3637
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 9,747,702 bp (GRCm38)
  • G to T, chromosome 1 at 62,166,369 bp (GRCm38)
  • T to A, chromosome 1 at 98,499,894 bp (GRCm38)
  • T to C, chromosome 1 at 104,947,336 bp (GRCm38)
  • A to T, chromosome 1 at 131,872,122 bp (GRCm38)
  • T to C, chromosome 1 at 161,856,858 bp (GRCm38)
  • A to G, chromosome 1 at 163,964,520 bp (GRCm38)
  • G to A, chromosome 1 at 164,049,406 bp (GRCm38)
  • T to C, chromosome 1 at 166,632,712 bp (GRCm38)
  • T to C, chromosome 2 at 17,348,867 bp (GRCm38)
  • T to A, chromosome 2 at 26,397,928 bp (GRCm38)
  • T to C, chromosome 2 at 44,996,976 bp (GRCm38)
  • T to C, chromosome 2 at 52,263,696 bp (GRCm38)
  • T to A, chromosome 2 at 89,097,913 bp (GRCm38)
  • C to A, chromosome 2 at 101,696,727 bp (GRCm38)
  • A to G, chromosome 2 at 160,779,473 bp (GRCm38)
  • G to T, chromosome 2 at 161,039,261 bp (GRCm38)
  • T to C, chromosome 3 at 31,029,515 bp (GRCm38)
  • T to A, chromosome 3 at 93,397,077 bp (GRCm38)
  • C to T, chromosome 3 at 146,097,140 bp (GRCm38)
  • G to T, chromosome 4 at 43,632,404 bp (GRCm38)
  • C to T, chromosome 4 at 73,735,719 bp (GRCm38)
  • T to A, chromosome 4 at 108,338,313 bp (GRCm38)
  • T to A, chromosome 4 at 133,416,370 bp (GRCm38)
  • T to C, chromosome 4 at 133,686,314 bp (GRCm38)
  • T to C, chromosome 4 at 134,229,019 bp (GRCm38)
  • T to A, chromosome 4 at 148,469,377 bp (GRCm38)
  • G to A, chromosome 5 at 27,631,441 bp (GRCm38)
  • C to A, chromosome 5 at 27,725,644 bp (GRCm38)
  • G to T, chromosome 5 at 32,737,440 bp (GRCm38)
  • T to C, chromosome 5 at 43,695,146 bp (GRCm38)
  • T to C, chromosome 5 at 48,192,226 bp (GRCm38)
  • A to G, chromosome 5 at 137,032,256 bp (GRCm38)
  • T to C, chromosome 6 at 8,175,414 bp (GRCm38)
  • A to G, chromosome 6 at 28,795,937 bp (GRCm38)
  • A to T, chromosome 6 at 46,001,347 bp (GRCm38)
  • T to C, chromosome 6 at 113,474,249 bp (GRCm38)
  • T to A, chromosome 6 at 119,271,953 bp (GRCm38)
  • T to A, chromosome 7 at 27,242,061 bp (GRCm38)
  • A to T, chromosome 7 at 74,554,488 bp (GRCm38)
  • T to C, chromosome 7 at 81,829,237 bp (GRCm38)
  • T to C, chromosome 7 at 89,509,934 bp (GRCm38)
  • C to A, chromosome 7 at 106,971,471 bp (GRCm38)
  • T to C, chromosome 7 at 110,315,085 bp (GRCm38)
  • A to G, chromosome 7 at 112,351,713 bp (GRCm38)
  • C to A, chromosome 7 at 128,142,260 bp (GRCm38)
  • C to A, chromosome 8 at 48,555,900 bp (GRCm38)
  • C to A, chromosome 8 at 84,536,417 bp (GRCm38)
  • T to C, chromosome 8 at 116,972,120 bp (GRCm38)
  • C to T, chromosome 8 at 123,234,134 bp (GRCm38)
  • A to T, chromosome 9 at 44,253,408 bp (GRCm38)
  • T to C, chromosome 9 at 58,249,662 bp (GRCm38)
  • C to T, chromosome 10 at 37,136,491 bp (GRCm38)
  • A to T, chromosome 10 at 92,962,315 bp (GRCm38)
  • T to A, chromosome 10 at 129,570,818 bp (GRCm38)
  • A to T, chromosome 10 at 130,392,124 bp (GRCm38)
  • A to T, chromosome 11 at 31,028,962 bp (GRCm38)
  • A to G, chromosome 11 at 34,294,139 bp (GRCm38)
  • A to G, chromosome 11 at 67,254,744 bp (GRCm38)
  • A to T, chromosome 11 at 76,814,781 bp (GRCm38)
  • A to G, chromosome 11 at 77,113,705 bp (GRCm38)
  • G to A, chromosome 11 at 85,051,202 bp (GRCm38)
  • T to C, chromosome 11 at 108,407,481 bp (GRCm38)
  • G to A, chromosome 12 at 30,002,052 bp (GRCm38)
  • T to C, chromosome 12 at 75,722,600 bp (GRCm38)
  • T to C, chromosome 12 at 103,732,497 bp (GRCm38)
  • T to C, chromosome 12 at 104,149,554 bp (GRCm38)
  • T to C, chromosome 12 at 111,099,959 bp (GRCm38)
  • T to G, chromosome 12 at 111,805,051 bp (GRCm38)
  • A to T, chromosome 13 at 12,438,610 bp (GRCm38)
  • A to G, chromosome 13 at 33,035,540 bp (GRCm38)
  • A to G, chromosome 13 at 33,833,221 bp (GRCm38)
  • A to C, chromosome 13 at 45,568,018 bp (GRCm38)
  • A to T, chromosome 13 at 67,176,446 bp (GRCm38)
  • G to A, chromosome 13 at 75,090,072 bp (GRCm38)
  • T to C, chromosome 14 at 35,026,805 bp (GRCm38)
  • T to G, chromosome 14 at 59,235,250 bp (GRCm38)
  • T to C, chromosome 15 at 28,448,433 bp (GRCm38)
  • G to A, chromosome 15 at 59,346,218 bp (GRCm38)
  • T to C, chromosome 15 at 66,827,269 bp (GRCm38)
  • G to A, chromosome 15 at 68,084,401 bp (GRCm38)
  • C to T, chromosome 15 at 75,948,100 bp (GRCm38)
  • G to A, chromosome 15 at 102,296,850 bp (GRCm38)
  • T to C, chromosome 16 at 3,715,388 bp (GRCm38)
  • T to A, chromosome 16 at 13,401,090 bp (GRCm38)
  • C to G, chromosome 17 at 32,189,316 bp (GRCm38)
  • A to C, chromosome 17 at 32,820,759 bp (GRCm38)
  • A to T, chromosome 17 at 35,155,999 bp (GRCm38)
  • G to A, chromosome 17 at 35,961,729 bp (GRCm38)
  • A to T, chromosome 17 at 56,955,598 bp (GRCm38)
  • A to G, chromosome 18 at 9,996,239 bp (GRCm38)
  • C to T, chromosome 18 at 34,357,169 bp (GRCm38)
  • T to A, chromosome 18 at 61,692,263 bp (GRCm38)
  • A to G, chromosome 19 at 4,086,464 bp (GRCm38)
  • T to C, chromosome 19 at 27,849,929 bp (GRCm38)
  • T to C, chromosome Y at 727,634 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9310 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069125-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.