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Strain Name:
C57BL/6J-MtgxR9345Btlr/Mmmh
Stock Number:
069156-MU
Citation ID:
RRID:MMRRC_069156-MU
Other Names:
R9345 (G1)
Major Collection:

Strain Information

Pofut2
Name: protein O-fucosyltransferase 2
Synonyms: FUT13, 2310011G23Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 80294
VEGA: 10
Homologene: 12724
Ppp1r14c
Name: protein phosphatase 1, regulatory inhibitor subunit 14C
Synonyms: 6330514J04Rik, KEPI
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 76142
VEGA: 10
Homologene: 12805
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Tnpo3
Name: transportin 3
Synonyms: 5730544L10Rik, C430013M08Rik, D6Ertd313e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 320938
Homologene: 40848
Fryl
Name: FRY like transcription coactivator
Synonyms: 2310004H21Rik, 2510002A14Rik, 9030227G01Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72313
Homologene: 103956
Dock5
Name: dedicator of cytokinesis 5
Synonyms: lr2, 1110060D06Rik, rlc
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 68813
VEGA: 14
Homologene: 57016
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to T, chromosome 1 at 24,685,512 bp (GRCm38)
  • A to G, chromosome 2 at 76,708,251 bp (GRCm38)
  • A to G, chromosome 2 at 85,399,753 bp (GRCm38)
  • T to A, chromosome 2 at 153,150,984 bp (GRCm38)
  • T to C, chromosome 2 at 165,812,748 bp (GRCm38)
  • T to A, chromosome 2 at 173,568,817 bp (GRCm38)
  • G to A, chromosome 3 at 83,128,794 bp (GRCm38)
  • T to A, chromosome 3 at 88,354,541 bp (GRCm38)
  • A to T, chromosome 3 at 105,658,687 bp (GRCm38)
  • C to A, chromosome 4 at 11,199,420 bp (GRCm38)
  • C to T, chromosome 4 at 115,610,502 bp (GRCm38)
  • C to T, chromosome 4 at 117,872,494 bp (GRCm38)
  • T to C, chromosome 4 at 147,882,192 bp (GRCm38)
  • T to G, chromosome 4 at 147,986,061 bp (GRCm38)
  • T to A, chromosome 4 at 150,470,313 bp (GRCm38)
  • T to G, chromosome 5 at 31,137,134 bp (GRCm38)
  • C to T, chromosome 5 at 73,050,411 bp (GRCm38)
  • T to C, chromosome 5 at 103,606,655 bp (GRCm38)
  • A to G, chromosome 5 at 122,104,839 bp (GRCm38)
  • A to T, chromosome 5 at 142,161,953 bp (GRCm38)
  • A to T, chromosome 5 at 146,514,520 bp (GRCm38)
  • T to C, chromosome 6 at 29,558,852 bp (GRCm38)
  • G to T, chromosome 6 at 141,547,827 bp (GRCm38)
  • A to T, chromosome 6 at 145,246,716 bp (GRCm38)
  • T to C, chromosome 7 at 18,565,156 bp (GRCm38)
  • A to C, chromosome 7 at 21,012,109 bp (GRCm38)
  • A to T, chromosome 7 at 28,316,987 bp (GRCm38)
  • A to G, chromosome 7 at 34,013,297 bp (GRCm38)
  • T to C, chromosome 7 at 44,306,765 bp (GRCm38)
  • T to C, chromosome 7 at 97,417,119 bp (GRCm38)
  • CGGC to CGGCGGCGGGGGC, chromosome 7 at 97,579,932 bp (GRCm38)
  • C to T, chromosome 7 at 103,098,506 bp (GRCm38)
  • A to G, chromosome 7 at 128,189,307 bp (GRCm38)
  • T to C, chromosome 8 at 3,459,363 bp (GRCm38)
  • A to G, chromosome 9 at 13,281,401 bp (GRCm38)
  • T to A, chromosome 9 at 52,116,710 bp (GRCm38)
  • T to A, chromosome 9 at 79,633,735 bp (GRCm38)
  • A to G, chromosome 9 at 106,651,088 bp (GRCm38)
  • C to A, chromosome 10 at 3,423,567 bp (GRCm38)
  • T to C, chromosome 10 at 61,548,111 bp (GRCm38)
  • A to G, chromosome 10 at 69,926,069 bp (GRCm38)
  • A to G, chromosome 10 at 77,267,256 bp (GRCm38)
  • A to T, chromosome 10 at 86,759,225 bp (GRCm38)
  • A to G, chromosome 11 at 3,492,170 bp (GRCm38)
  • T to A, chromosome 11 at 43,203,197 bp (GRCm38)
  • A to G, chromosome 11 at 74,899,300 bp (GRCm38)
  • A to G, chromosome 11 at 75,513,575 bp (GRCm38)
  • A to G, chromosome 11 at 107,080,762 bp (GRCm38)
  • A to T, chromosome 11 at 120,815,909 bp (GRCm38)
  • A to G, chromosome 11 at 121,573,822 bp (GRCm38)
  • T to A, chromosome 12 at 3,983,409 bp (GRCm38)
  • A to G, chromosome 12 at 21,328,055 bp (GRCm38)
  • G to A, chromosome 12 at 40,217,459 bp (GRCm38)
  • A to G, chromosome 12 at 84,057,092 bp (GRCm38)
  • C to A, chromosome 12 at 84,147,458 bp (GRCm38)
  • G to A, chromosome 13 at 23,162,599 bp (GRCm38)
  • A to G, chromosome 13 at 70,592,639 bp (GRCm38)
  • A to T, chromosome 14 at 4,398,488 bp (GRCm38)
  • C to T, chromosome 14 at 12,296,641 bp (GRCm38)
  • G to A, chromosome 14 at 21,780,159 bp (GRCm38)
  • T to C, chromosome 14 at 67,822,622 bp (GRCm38)
  • T to C, chromosome 14 at 99,081,378 bp (GRCm38)
  • G to A, chromosome 15 at 99,279,735 bp (GRCm38)
  • A to T, chromosome 17 at 34,921,243 bp (GRCm38)
  • C to T, chromosome 17 at 71,274,544 bp (GRCm38)
  • A to G, chromosome 18 at 34,626,726 bp (GRCm38)
  • A to G, chromosome 18 at 37,448,228 bp (GRCm38)
  • T to C, chromosome 18 at 50,166,914 bp (GRCm38)
  • A to T, chromosome 19 at 43,819,430 bp (GRCm38)
  • T to C, chromosome 19 at 55,194,665 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9345 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069156-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.