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Strain Name:
C57BL/6J-MtgxR9384Btlr/Mmmh
Stock Number:
069191-MU
Citation ID:
RRID:MMRRC_069191-MU
Other Names:
R9384 (G1)
Major Collection:

Strain Information

Slc12a8
Name: solute carrier family 12 (potassium/chloride transporters), member 8
Synonyms: E330020C02Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 171286
Homologene: 11628
Arhgef10
Name: Rho guanine nucleotide exchange factor 10
Synonyms: 6430549H08Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234094
Homologene: 22827
Fzd6
Name: frizzled class receptor 6
Synonyms: Fz6, rst
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 14368
VEGA: 15
HGNC: HGNC:4044
Homologene: 2617
Agrn
Name: agrin
Synonyms: NMF380, Agrin, nmf380
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11603
HGNC: HGNC:329
Homologene: 27907
Rtn4
Name: reticulon 4
Synonyms: 1110020G17Rik, C130026I10Rik, NOGO, NgA, Nogo-B, Nogo-A
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 68585
Homologene: 10743
Sugt1
Name: SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Synonyms: SGT1, 2410174K12Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 67955
VEGA: 14
Homologene: 4877
Tmprss6
Name: transmembrane serine protease 6
Synonyms: matriptase-2, 1300008A22Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 71753
VEGA: 15
Homologene: 12408
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 43,056,752 bp (GRCm38)
  • C to A, chromosome 1 at 111,860,133 bp (GRCm38)
  • A to T, chromosome 2 at 51,628,022 bp (GRCm38)
  • T to A, chromosome 2 at 86,677,013 bp (GRCm38)
  • A to G, chromosome 2 at 104,257,575 bp (GRCm38)
  • A to C, chromosome 3 at 55,247,515 bp (GRCm38)
  • A to G, chromosome 4 at 102,255,251 bp (GRCm38)
  • G to A, chromosome 4 at 128,762,058 bp (GRCm38)
  • A to G, chromosome 4 at 139,467,320 bp (GRCm38)
  • C to T, chromosome 4 at 156,172,649 bp (GRCm38)
  • T to G, chromosome 5 at 39,614,619 bp (GRCm38)
  • C to A, chromosome 5 at 109,053,400 bp (GRCm38)
  • A to T, chromosome 5 at 123,864,534 bp (GRCm38)
  • T to C, chromosome 5 at 149,214,539 bp (GRCm38)
  • C to T, chromosome 6 at 29,496,619 bp (GRCm38)
  • A to T, chromosome 6 at 55,975,628 bp (GRCm38)
  • G to T, chromosome 6 at 70,059,190 bp (GRCm38)
  • A to G, chromosome 6 at 71,897,126 bp (GRCm38)
  • T to G, chromosome 6 at 123,315,964 bp (GRCm38)
  • T to C, chromosome 6 at 124,778,226 bp (GRCm38)
  • T to A, chromosome 7 at 12,494,008 bp (GRCm38)
  • T to A, chromosome 7 at 26,558,733 bp (GRCm38)
  • T to C, chromosome 7 at 88,074,310 bp (GRCm38)
  • T to C, chromosome 7 at 131,302,675 bp (GRCm38)
  • T to C, chromosome 8 at 14,991,067 bp (GRCm38)
  • A to G, chromosome 8 at 45,805,827 bp (GRCm38)
  • G to T, chromosome 8 at 57,962,427 bp (GRCm38)
  • T to C, chromosome 9 at 51,272,338 bp (GRCm38)
  • T to C, chromosome 9 at 107,534,543 bp (GRCm38)
  • A to G, chromosome 10 at 77,787,780 bp (GRCm38)
  • T to A, chromosome 10 at 103,170,597 bp (GRCm38)
  • T to A, chromosome 10 at 109,718,297 bp (GRCm38)
  • T to C, chromosome 11 at 29,708,471 bp (GRCm38)
  • C to A, chromosome 11 at 73,786,970 bp (GRCm38)
  • A to G, chromosome 11 at 77,469,392 bp (GRCm38)
  • A to T, chromosome 11 at 78,078,304 bp (GRCm38)
  • A to G, chromosome 14 at 48,486,819 bp (GRCm38)
  • T to C, chromosome 14 at 79,628,948 bp (GRCm38)
  • C to A, chromosome 15 at 39,031,707 bp (GRCm38)
  • T to G, chromosome 15 at 39,031,708 bp (GRCm38)
  • G to A, chromosome 15 at 78,444,102 bp (GRCm38)
  • A to G, chromosome 15 at 85,066,254 bp (GRCm38)
  • G to T, chromosome 15 at 86,033,085 bp (GRCm38)
  • T to A, chromosome 16 at 33,646,577 bp (GRCm38)
  • C to T, chromosome 16 at 58,465,563 bp (GRCm38)
  • A to T, chromosome 17 at 43,626,892 bp (GRCm38)
  • T to A, chromosome 18 at 37,504,971 bp (GRCm38)
  • A to T, chromosome 18 at 86,395,840 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9384 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069191-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.