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Strain Name:
C57BL/6J-MtgxR9408Btlr/Mmmh
Stock Number:
069212-MU
Citation ID:
RRID:MMRRC_069212-MU
Other Names:
R9408 (G1)
Major Collection:

Strain Information

Gnb5
Name: guanine nucleotide binding protein (G protein), beta 5
Synonyms: G beta 5, Gbeta5
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 14697
VEGA: 9
HGNC: HGNC:4401
Homologene: 40714
Sbf2
Name: SET binding factor 2
Synonyms: SBF2, 4833411B01Rik, mMTMH1, B430219L04Rik, Mtmr13
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 319934
HGNC: HGNC:2135
Homologene: 41810
Cacna1g
Name: calcium channel, voltage-dependent, T type, alpha 1G subunit
Synonyms: a1G, Cav3.1d
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12291
HGNC: HGNC:1394
Homologene: 22544
Uri1
Name: URI1, prefoldin-like chaperone
Synonyms: NNX3, Rmp, C80913
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 19777
Homologene: 2813
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 29808
Homologene: 49351
Sash1
Name: SAM and SH3 domain containing 1
Synonyms: A330076K04Rik, 1100001C18Rik, 2500002E12Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 70097
Homologene: 69182
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 75,201,039 bp (GRCm38)
  • T to C, chromosome 1 at 92,917,702 bp (GRCm38)
  • C to A, chromosome 1 at 167,314,131 bp (GRCm38)
  • T to C, chromosome 1 at 174,050,763 bp (GRCm38)
  • C to A, chromosome 1 at 179,776,073 bp (GRCm38)
  • A to G, chromosome 2 at 26,848,275 bp (GRCm38)
  • T to A, chromosome 2 at 32,047,511 bp (GRCm38)
  • A to C, chromosome 2 at 70,786,875 bp (GRCm38)
  • G to A, chromosome 2 at 87,868,035 bp (GRCm38)
  • A to G, chromosome 2 at 89,630,044 bp (GRCm38)
  • T to A, chromosome 2 at 119,935,518 bp (GRCm38)
  • T to A, chromosome 2 at 167,989,318 bp (GRCm38)
  • T to A, chromosome 3 at 10,089,347 bp (GRCm38)
  • A to C, chromosome 3 at 19,233,794 bp (GRCm38)
  • T to C, chromosome 3 at 58,460,032 bp (GRCm38)
  • T to A, chromosome 3 at 60,039,565 bp (GRCm38)
  • G to A, chromosome 3 at 83,285,266 bp (GRCm38)
  • A to T, chromosome 3 at 122,137,625 bp (GRCm38)
  • T to G, chromosome 4 at 21,746,776 bp (GRCm38)
  • C to A, chromosome 4 at 33,246,488 bp (GRCm38)
  • T to A, chromosome 4 at 45,279,182 bp (GRCm38)
  • T to A, chromosome 4 at 49,447,773 bp (GRCm38)
  • A to G, chromosome 5 at 8,397,764 bp (GRCm38)
  • A to G, chromosome 5 at 150,541,517 bp (GRCm38)
  • A to G, chromosome 6 at 124,320,538 bp (GRCm38)
  • G to A, chromosome 6 at 128,545,067 bp (GRCm38)
  • C to T, chromosome 6 at 134,887,741 bp (GRCm38)
  • A to G, chromosome 7 at 5,544,919 bp (GRCm38)
  • A to T, chromosome 7 at 16,072,214 bp (GRCm38)
  • A to T, chromosome 7 at 23,577,917 bp (GRCm38)
  • G to A, chromosome 7 at 25,271,989 bp (GRCm38)
  • A to T, chromosome 7 at 37,969,606 bp (GRCm38)
  • A to T, chromosome 7 at 46,267,297 bp (GRCm38)
  • A to T, chromosome 7 at 102,949,236 bp (GRCm38)
  • T to C, chromosome 7 at 110,441,495 bp (GRCm38)
  • A to T, chromosome 7 at 131,544,373 bp (GRCm38)
  • A to G, chromosome 7 at 135,115,336 bp (GRCm38)
  • T to A, chromosome 8 at 48,137,350 bp (GRCm38)
  • G to A, chromosome 9 at 15,333,323 bp (GRCm38)
  • T to C, chromosome 9 at 46,275,449 bp (GRCm38)
  • T to A, chromosome 9 at 75,311,414 bp (GRCm38)
  • C to A, chromosome 9 at 108,139,453 bp (GRCm38)
  • T to A, chromosome 9 at 109,486,033 bp (GRCm38)
  • T to A, chromosome 9 at 122,797,663 bp (GRCm38)
  • AC to A, chromosome 10 at 4,450,848 bp (GRCm38)
  • G to A, chromosome 10 at 8,762,230 bp (GRCm38)
  • A to G, chromosome 10 at 14,131,761 bp (GRCm38)
  • T to A, chromosome 10 at 21,150,376 bp (GRCm38)
  • A to T, chromosome 10 at 23,123,907 bp (GRCm38)
  • A to G, chromosome 10 at 78,984,386 bp (GRCm38)
  • A to T, chromosome 10 at 86,300,918 bp (GRCm38)
  • G to A, chromosome 10 at 107,485,666 bp (GRCm38)
  • A to T, chromosome 11 at 36,069,419 bp (GRCm38)
  • T to A, chromosome 11 at 50,969,786 bp (GRCm38)
  • T to A, chromosome 11 at 72,864,827 bp (GRCm38)
  • T to C, chromosome 11 at 78,339,387 bp (GRCm38)
  • T to C, chromosome 11 at 80,155,116 bp (GRCm38)
  • T to G, chromosome 11 at 94,430,224 bp (GRCm38)
  • C to T, chromosome 11 at 102,107,285 bp (GRCm38)
  • T to A, chromosome 11 at 104,730,429 bp (GRCm38)
  • A to G, chromosome 13 at 100,655,495 bp (GRCm38)
  • A to G, chromosome 13 at 104,209,647 bp (GRCm38)
  • A to G, chromosome 14 at 50,837,180 bp (GRCm38)
  • G to A, chromosome 14 at 51,888,034 bp (GRCm38)
  • T to C, chromosome 15 at 27,591,502 bp (GRCm38)
  • A to C, chromosome 15 at 102,553,851 bp (GRCm38)
  • A to T, chromosome 16 at 26,714,925 bp (GRCm38)
  • G to T, chromosome 16 at 38,526,775 bp (GRCm38)
  • A to T, chromosome 16 at 78,547,758 bp (GRCm38)
  • C to T, chromosome 16 at 90,944,852 bp (GRCm38)
  • A to G, chromosome 17 at 22,454,588 bp (GRCm38)
  • G to T, chromosome 17 at 24,474,109 bp (GRCm38)
  • G to A, chromosome 17 at 34,801,886 bp (GRCm38)
  • T to C, chromosome 18 at 34,430,330 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9408 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069212-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.