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Strain Name:
C57BL/6J-MtgxR9433Btlr/Mmmh
Stock Number:
069237-MU
Citation ID:
RRID:MMRRC_069237-MU
Other Names:
R9433 (G1)
Major Collection:

Strain Information

Sufu
Name: SUFU negative regulator of hedgehog signaling
Synonyms: Su(Fu), 2810026F04Rik, b2b273Clo
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 24069
Homologene: 9262
Cnnm2
Name: cyclin M2
Synonyms: Acdp2
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 94219
VEGA: 19
HGNC: HGNC:103
Homologene: 9761
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: iNOS, Nos-2, NOS-II, Nos2a
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Etv5
Name: ets variant 5
Synonyms: 8430401F14Rik, erm, 1110005E01Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 104156
HGNC: HGNC:3494
Homologene: 3276
Plxnd1
Name: plexin D1
Synonyms: 6230425C21Rik, b2b553Clo, b2b1863Clo
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67784
HGNC: HGNC:9107
Homologene: 22866
Adipor2
Name: adiponectin receptor 2
Synonyms: 1110001I14Rik, D6Ucla1e
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 68465
Homologene: 56119
Hcn4
Name: hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 330953
VEGA: 9
Homologene: 3997
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to C, chromosome 1 at 38,053,092 bp (GRCm38)
  • G to A, chromosome 1 at 38,836,457 bp (GRCm38)
  • A to T, chromosome 1 at 44,056,508 bp (GRCm38)
  • A to G, chromosome 1 at 87,125,795 bp (GRCm38)
  • A to G, chromosome 1 at 95,627,639 bp (GRCm38)
  • C to T, chromosome 1 at 171,174,429 bp (GRCm38)
  • A to G, chromosome 2 at 5,675,966 bp (GRCm38)
  • A to T, chromosome 2 at 70,593,463 bp (GRCm38)
  • T to A, chromosome 2 at 152,736,510 bp (GRCm38)
  • C to T, chromosome 2 at 165,112,409 bp (GRCm38)
  • C to T, chromosome 2 at 181,855,044 bp (GRCm38)
  • A to T, chromosome 3 at 83,938,152 bp (GRCm38)
  • T to A, chromosome 3 at 103,321,663 bp (GRCm38)
  • C to T, chromosome 3 at 104,015,157 bp (GRCm38)
  • A to G, chromosome 3 at 107,668,329 bp (GRCm38)
  • T to C, chromosome 4 at 88,644,303 bp (GRCm38)
  • T to A, chromosome 4 at 96,664,007 bp (GRCm38)
  • A to G, chromosome 4 at 112,280,235 bp (GRCm38)
  • T to A, chromosome 5 at 33,152,681 bp (GRCm38)
  • T to A, chromosome 5 at 145,108,393 bp (GRCm38)
  • C to T, chromosome 6 at 42,305,560 bp (GRCm38)
  • G to A, chromosome 6 at 87,903,496 bp (GRCm38)
  • T to C, chromosome 6 at 115,816,034 bp (GRCm38)
  • G to T, chromosome 6 at 115,968,793 bp (GRCm38)
  • A to T, chromosome 6 at 119,359,525 bp (GRCm38)
  • A to G, chromosome 7 at 42,046,166 bp (GRCm38)
  • G to A, chromosome 7 at 44,312,918 bp (GRCm38)
  • T to A, chromosome 7 at 85,155,709 bp (GRCm38)
  • T to A, chromosome 7 at 116,083,636 bp (GRCm38)
  • A to G, chromosome 7 at 127,485,100 bp (GRCm38)
  • C to T, chromosome 7 at 127,934,167 bp (GRCm38)
  • G to T, chromosome 8 at 22,561,195 bp (GRCm38)
  • A to T, chromosome 8 at 71,716,824 bp (GRCm38)
  • A to T, chromosome 8 at 95,545,985 bp (GRCm38)
  • C to T, chromosome 8 at 111,081,690 bp (GRCm38)
  • T to A, chromosome 9 at 18,572,641 bp (GRCm38)
  • A to T, chromosome 9 at 57,056,726 bp (GRCm38)
  • A to G, chromosome 9 at 58,823,939 bp (GRCm38)
  • G to T, chromosome 9 at 87,019,662 bp (GRCm38)
  • G to A, chromosome 10 at 33,919,359 bp (GRCm38)
  • T to A, chromosome 10 at 58,270,770 bp (GRCm38)
  • T to C, chromosome 10 at 70,169,121 bp (GRCm38)
  • T to A, chromosome 10 at 81,591,046 bp (GRCm38)
  • A to C, chromosome 10 at 88,139,996 bp (GRCm38)
  • A to G, chromosome 10 at 128,276,788 bp (GRCm38)
  • C to T, chromosome 10 at 130,446,698 bp (GRCm38)
  • A to T, chromosome 11 at 6,836,902 bp (GRCm38)
  • A to T, chromosome 11 at 60,204,189 bp (GRCm38)
  • G to A, chromosome 11 at 78,959,664 bp (GRCm38)
  • A to G, chromosome 11 at 100,875,044 bp (GRCm38)
  • T to C, chromosome 12 at 44,283,522 bp (GRCm38)
  • G to A, chromosome 12 at 55,866,078 bp (GRCm38)
  • A to G, chromosome 12 at 59,101,585 bp (GRCm38)
  • T to C, chromosome 12 at 81,419,949 bp (GRCm38)
  • A to G, chromosome 12 at 101,981,993 bp (GRCm38)
  • T to C, chromosome 12 at 118,012,272 bp (GRCm38)
  • C to T, chromosome 13 at 21,611,054 bp (GRCm38)
  • A to G, chromosome 13 at 33,334,255 bp (GRCm38)
  • A to T, chromosome 13 at 43,422,565 bp (GRCm38)
  • A to T, chromosome 13 at 81,566,352 bp (GRCm38)
  • A to G, chromosome 14 at 20,561,610 bp (GRCm38)
  • C to T, chromosome 14 at 31,143,574 bp (GRCm38)
  • T to A, chromosome 14 at 61,206,548 bp (GRCm38)
  • C to A, chromosome 14 at 62,365,273 bp (GRCm38)
  • T to C, chromosome 14 at 79,098,431 bp (GRCm38)
  • A to G, chromosome 15 at 31,592,737 bp (GRCm38)
  • C to T, chromosome 15 at 79,028,747 bp (GRCm38)
  • T to C, chromosome 15 at 85,819,869 bp (GRCm38)
  • G to T, chromosome 15 at 99,178,565 bp (GRCm38)
  • G to A, chromosome 16 at 22,435,918 bp (GRCm38)
  • T to A, chromosome 16 at 22,565,315 bp (GRCm38)
  • A to G, chromosome 16 at 46,035,935 bp (GRCm38)
  • C to G, chromosome 16 at 87,719,715 bp (GRCm38)
  • A to G, chromosome 17 at 5,731,504 bp (GRCm38)
  • G to A, chromosome 17 at 27,118,677 bp (GRCm38)
  • G to A, chromosome 17 at 78,624,984 bp (GRCm38)
  • C to A, chromosome 18 at 46,290,973 bp (GRCm38)
  • A to G, chromosome 19 at 46,397,093 bp (GRCm38)
  • C to T, chromosome 19 at 46,762,368 bp (GRCm38)
  • A to T, chromosome 19 at 47,267,100 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9433 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069237-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.