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Strain Name:
C57BL/6J-MtgxR9443Btlr/Mmmh
Stock Number:
069247-MU
Citation ID:
RRID:MMRRC_069247-MU
Other Names:
R9443 (G1)
Major Collection:

Strain Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231051
Homologene: 46480
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: 9430004D19Rik, C030040A15Rik, OTTMUSG00000012483
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 109241
Homologene: 81861
Eomes
Name: eomesodermin
Synonyms: Tbr2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 13813
HGNC: HGNC:3372
Homologene: 3971
Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Synonyms: M1, muscarinic acetylcholine receptor 1, Chrm-1, M1R, AW495047
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 12669
HGNC: HGNC:1950
Homologene: 20189
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Osbpl1a
Name: oxysterol binding protein-like 1A
Synonyms: LOC328902, G430090F17Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 64291
Homologene: 84746
Ndufv1
Name: NADH:ubiquinone oxidoreductase core subunit V1
Synonyms: 24 kDa (FP)
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17995
HGNC: HGNC:7716
Homologene: 5151
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • C to A, chromosome 1 at 4,912,406 bp (GRCm38)
  • T to C, chromosome 1 at 82,768,369 bp (GRCm38)
  • T to A, chromosome 1 at 185,235,326 bp (GRCm38)
  • A to G, chromosome 1 at 192,101,750 bp (GRCm38)
  • C to T, chromosome 2 at 34,838,611 bp (GRCm38)
  • T to C, chromosome 2 at 34,946,736 bp (GRCm38)
  • T to G, chromosome 2 at 49,256,700 bp (GRCm38)
  • T to C, chromosome 2 at 66,565,209 bp (GRCm38)
  • T to A, chromosome 2 at 84,667,180 bp (GRCm38)
  • T to A, chromosome 2 at 88,340,020 bp (GRCm38)
  • T to C, chromosome 2 at 128,762,109 bp (GRCm38)
  • C to T, chromosome 2 at 148,847,958 bp (GRCm38)
  • A to T, chromosome 2 at 152,004,379 bp (GRCm38)
  • T to A, chromosome 2 at 180,201,729 bp (GRCm38)
  • A to T, chromosome 3 at 19,978,919 bp (GRCm38)
  • T to C, chromosome 3 at 37,091,991 bp (GRCm38)
  • C to A, chromosome 3 at 89,467,649 bp (GRCm38)
  • T to A, chromosome 3 at 135,270,848 bp (GRCm38)
  • A to G, chromosome 4 at 43,696,591 bp (GRCm38)
  • C to T, chromosome 4 at 58,179,697 bp (GRCm38)
  • C to T, chromosome 4 at 117,871,158 bp (GRCm38)
  • T to A, chromosome 4 at 123,471,875 bp (GRCm38)
  • T to A, chromosome 4 at 144,333,108 bp (GRCm38)
  • A to G, chromosome 5 at 25,310,047 bp (GRCm38)
  • A to T, chromosome 5 at 109,047,293 bp (GRCm38)
  • T to C, chromosome 6 at 30,750,579 bp (GRCm38)
  • C to T, chromosome 6 at 124,451,399 bp (GRCm38)
  • T to A, chromosome 6 at 136,634,557 bp (GRCm38)
  • T to C, chromosome 7 at 30,104,824 bp (GRCm38)
  • A to T, chromosome 7 at 30,977,642 bp (GRCm38)
  • T to C, chromosome 7 at 104,703,614 bp (GRCm38)
  • T to C, chromosome 7 at 139,615,107 bp (GRCm38)
  • C to T, chromosome 7 at 143,085,123 bp (GRCm38)
  • A to T, chromosome 8 at 95,328,294 bp (GRCm38)
  • A to G, chromosome 8 at 121,797,026 bp (GRCm38)
  • T to C, chromosome 8 at 123,126,672 bp (GRCm38)
  • T to A, chromosome 9 at 42,659,741 bp (GRCm38)
  • T to C, chromosome 9 at 62,425,798 bp (GRCm38)
  • G to A, chromosome 9 at 108,955,991 bp (GRCm38)
  • T to A, chromosome 9 at 110,320,936 bp (GRCm38)
  • A to G, chromosome 9 at 118,484,572 bp (GRCm38)
  • C to T, chromosome 10 at 13,247,097 bp (GRCm38)
  • A to G, chromosome 10 at 24,050,413 bp (GRCm38)
  • G to A, chromosome 10 at 42,313,591 bp (GRCm38)
  • A to G, chromosome 10 at 75,926,899 bp (GRCm38)
  • T to C, chromosome 10 at 81,317,350 bp (GRCm38)
  • T to C, chromosome 11 at 34,205,671 bp (GRCm38)
  • C to T, chromosome 11 at 69,940,362 bp (GRCm38)
  • G to T, chromosome 11 at 70,649,951 bp (GRCm38)
  • T to G, chromosome 11 at 80,132,562 bp (GRCm38)
  • T to C, chromosome 11 at 101,764,914 bp (GRCm38)
  • T to C, chromosome 12 at 88,371,780 bp (GRCm38)
  • T to A, chromosome 12 at 103,070,776 bp (GRCm38)
  • T to C, chromosome 13 at 65,296,533 bp (GRCm38)
  • A to G, chromosome 13 at 76,118,169 bp (GRCm38)
  • A to T, chromosome 14 at 13,682,364 bp (GRCm38)
  • T to C, chromosome 15 at 4,944,339 bp (GRCm38)
  • T to A, chromosome 15 at 76,434,764 bp (GRCm38)
  • C to T, chromosome 15 at 78,881,047 bp (GRCm38)
  • T to C, chromosome 15 at 88,925,660 bp (GRCm38)
  • T to C, chromosome 15 at 97,802,471 bp (GRCm38)
  • T to C, chromosome 15 at 99,601,528 bp (GRCm38)
  • C to T, chromosome 16 at 91,495,479 bp (GRCm38)
  • T to G, chromosome 17 at 27,105,549 bp (GRCm38)
  • C to T, chromosome 17 at 33,382,444 bp (GRCm38)
  • A to G, chromosome 17 at 35,080,850 bp (GRCm38)
  • A to G, chromosome 17 at 56,662,720 bp (GRCm38)
  • T to A, chromosome 18 at 3,434,041 bp (GRCm38)
  • C to A, chromosome 18 at 12,898,187 bp (GRCm38)
  • C to A, chromosome 18 at 38,197,580 bp (GRCm38)
  • T to C, chromosome 18 at 61,746,788 bp (GRCm38)
  • A to G, chromosome 18 at 63,720,336 bp (GRCm38)
  • C to A, chromosome 19 at 4,007,614 bp (GRCm38)
  • A to T, chromosome 19 at 8,678,186 bp (GRCm38)
  • G to T, chromosome X at 99,976,169 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9443 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069247-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.