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Strain Name:
C57BL/6J-MtgxR9478Btlr/Mmmh
Stock Number:
069281-MU
Citation ID:
RRID:MMRRC_069281-MU
Other Names:
R9478 (G1)
Major Collection:

Strain Information

Sema3e
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Synonyms: Semah
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20349
Homologene: 8247
Vps41
Name: VPS41 HOPS complex subunit
Synonyms: Vam2, mVam2
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 218035
VEGA: 13
Homologene: 69165
Pank4
Name: pantothenate kinase 4
Synonyms: D030031I12Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 269614
Homologene: 41235
Hgf
Name: hepatocyte growth factor
Synonyms: scatter factor, NK1, SF/HGF, HGF/SF, NK2, C230052L06Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 15234
HGNC: HGNC:4893
Homologene: 503
Zmynd8
Name: zinc finger, MYND-type containing 8
Synonyms: 1110013E22Rik, 2010005I16Rik, ZMYND8, RACK7, 3632413B07Rik, Prkcbp1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 228880
HGNC: HGNC:9397
Homologene: 32679
Esco2
Name: establishment of sister chromatid cohesion N-acetyltransferase 2
Synonyms: D030072L07Rik, 2410004I17Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 71988
Homologene: 12432
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to G, chromosome 1 at 4,347,322 bp (GRCm38)
  • T to C, chromosome 1 at 40,773,781 bp (GRCm38)
  • A to G, chromosome 1 at 59,655,456 bp (GRCm38)
  • A to G, chromosome 1 at 75,509,918 bp (GRCm38)
  • T to A, chromosome 2 at 52,188,776 bp (GRCm38)
  • T to C, chromosome 2 at 66,326,149 bp (GRCm38)
  • C to A, chromosome 2 at 90,149,251 bp (GRCm38)
  • G to A, chromosome 2 at 127,235,073 bp (GRCm38)
  • C to A, chromosome 2 at 165,807,649 bp (GRCm38)
  • T to A, chromosome 3 at 63,699,404 bp (GRCm38)
  • A to T, chromosome 3 at 101,818,671 bp (GRCm38)
  • A to T, chromosome 4 at 57,197,573 bp (GRCm38)
  • G to A, chromosome 4 at 84,979,393 bp (GRCm38)
  • A to G, chromosome 4 at 124,610,358 bp (GRCm38)
  • C to T, chromosome 4 at 135,965,263 bp (GRCm38)
  • G to T, chromosome 4 at 136,938,586 bp (GRCm38)
  • C to T, chromosome 4 at 149,261,159 bp (GRCm38)
  • G to A, chromosome 4 at 154,980,108 bp (GRCm38)
  • T to C, chromosome 5 at 14,236,372 bp (GRCm38)
  • A to G, chromosome 5 at 16,561,031 bp (GRCm38)
  • A to G, chromosome 5 at 24,565,942 bp (GRCm38)
  • T to A, chromosome 5 at 146,098,187 bp (GRCm38)
  • A to G, chromosome 6 at 120,474,375 bp (GRCm38)
  • T to C, chromosome 7 at 27,476,254 bp (GRCm38)
  • G to T, chromosome 7 at 29,198,352 bp (GRCm38)
  • G to A, chromosome 7 at 107,903,031 bp (GRCm38)
  • T to C, chromosome 7 at 127,584,915 bp (GRCm38)
  • T to A, chromosome 7 at 134,766,233 bp (GRCm38)
  • C to T, chromosome 8 at 116,954,798 bp (GRCm38)
  • C to T, chromosome 8 at 129,110,992 bp (GRCm38)
  • T to C, chromosome 9 at 53,391,936 bp (GRCm38)
  • C to G, chromosome 9 at 108,611,739 bp (GRCm38)
  • T to C, chromosome 9 at 123,708,102 bp (GRCm38)
  • T to A, chromosome 10 at 27,015,482 bp (GRCm38)
  • T to G, chromosome 10 at 53,348,779 bp (GRCm38)
  • C to A, chromosome 10 at 59,438,642 bp (GRCm38)
  • T to A, chromosome 10 at 129,351,091 bp (GRCm38)
  • A to G, chromosome 11 at 6,475,424 bp (GRCm38)
  • A to G, chromosome 11 at 73,077,942 bp (GRCm38)
  • A to G, chromosome 12 at 76,107,613 bp (GRCm38)
  • A to T, chromosome 12 at 104,412,413 bp (GRCm38)
  • T to C, chromosome 12 at 110,658,703 bp (GRCm38)
  • A to G, chromosome 13 at 18,862,743 bp (GRCm38)
  • G to T, chromosome 13 at 19,623,440 bp (GRCm38)
  • A to T, chromosome 13 at 77,303,454 bp (GRCm38)
  • G to A, chromosome 14 at 35,321,707 bp (GRCm38)
  • G to T, chromosome 14 at 47,545,681 bp (GRCm38)
  • A to T, chromosome 14 at 50,533,594 bp (GRCm38)
  • A to T, chromosome 14 at 52,744,597 bp (GRCm38)
  • C to A, chromosome 14 at 65,831,208 bp (GRCm38)
  • T to C, chromosome 14 at 118,115,132 bp (GRCm38)
  • T to C, chromosome 15 at 34,345,096 bp (GRCm38)
  • C to A, chromosome 15 at 78,284,765 bp (GRCm38)
  • G to A, chromosome 15 at 101,487,568 bp (GRCm38)
  • T to G, chromosome 16 at 20,123,013 bp (GRCm38)
  • T to C, chromosome 16 at 45,144,063 bp (GRCm38)
  • A to T, chromosome 17 at 7,334,341 bp (GRCm38)
  • G to A, chromosome 18 at 13,817,315 bp (GRCm38)
  • A to T, chromosome 18 at 20,397,951 bp (GRCm38)
  • G to A, chromosome 18 at 22,920,877 bp (GRCm38)
  • C to T, chromosome 19 at 7,271,429 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9478 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069281-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.