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Strain Name:
C57BL/6J-MtgxR9520Btlr/Mmmh
Stock Number:
069322-MU
Citation ID:
RRID:MMRRC_069322-MU
Other Names:
R9520 (G1)
Major Collection:

Strain Information

B4galnt1
Name: beta-1,4-N-acetyl-galactosaminyl transferase 1
Synonyms: Gal-NAc-T, GalNAcT, GM2/GD2 synthase, GalNAc-T, Ggm-2, Ggm2, 4933429D13Rik, Galgt1, beta1,4GalNAC-T
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 14421
VEGA: 10
HGNC: HGNC:4117
Homologene: 1133
Mtmr3
Name: myotubularin related protein 3
Synonyms: FYVE-DSP1, 1700092A20Rik, ZFYVE10
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74302
HGNC: HGNC:7451
Homologene: 23662
Prkacb
Name: protein kinase, cAMP dependent, catalytic, beta
Synonyms: cAMP-dependent protein kinase C beta, Pkacb
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 18749
HGNC: HGNC:9381
Homologene: 121718
Iqgap1
Name: IQ motif containing GTPase activating protein 1
Synonyms: D7Ertd237e, D7Ertd257e
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 29875
HGNC: HGNC:6110
Homologene: 74514
Rnf145
Name: ring finger protein 145
Synonyms: 3732413I11Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 74315
Homologene: 14427
Pck1
Name: phosphoenolpyruvate carboxykinase 1, cytosolic
Synonyms: Pck-1, PEPCK
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 18534
HGNC: HGNC:8724
Homologene: 1944
Sec23a
Name: SEC23 homolog A, COPII coat complex component
Synonyms: Sec23r, Msec23
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20334
Homologene: 4642
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 90,214,249 bp (GRCm38)
  • A to G, chromosome 1 at 139,754,397 bp (GRCm38)
  • A to G, chromosome 1 at 154,205,475 bp (GRCm38)
  • G to T, chromosome 1 at 181,209,022 bp (GRCm38)
  • G to A, chromosome 2 at 16,074,820 bp (GRCm38)
  • A to T, chromosome 2 at 24,761,787 bp (GRCm38)
  • A to G, chromosome 2 at 70,232,409 bp (GRCm38)
  • G to A, chromosome 2 at 173,156,061 bp (GRCm38)
  • A to T, chromosome 3 at 92,646,802 bp (GRCm38)
  • A to T, chromosome 3 at 103,137,168 bp (GRCm38)
  • T to C, chromosome 3 at 146,750,534 bp (GRCm38)
  • T to C, chromosome 4 at 81,386,318 bp (GRCm38)
  • T to A, chromosome 4 at 117,000,685 bp (GRCm38)
  • G to A, chromosome 4 at 137,613,685 bp (GRCm38)
  • T to C, chromosome 4 at 149,995,918 bp (GRCm38)
  • T to C, chromosome 4 at 152,311,777 bp (GRCm38)
  • T to C, chromosome 5 at 109,053,589 bp (GRCm38)
  • T to A, chromosome 5 at 115,941,895 bp (GRCm38)
  • C to T, chromosome 5 at 120,963,840 bp (GRCm38)
  • T to C, chromosome 5 at 124,090,113 bp (GRCm38)
  • T to C, chromosome 6 at 42,912,117 bp (GRCm38)
  • A to G, chromosome 6 at 70,960,692 bp (GRCm38)
  • A to T, chromosome 6 at 113,304,452 bp (GRCm38)
  • A to T, chromosome 6 at 123,853,107 bp (GRCm38)
  • T to C, chromosome 6 at 135,733,401 bp (GRCm38)
  • T to C, chromosome 7 at 13,008,085 bp (GRCm38)
  • T to C, chromosome 7 at 80,744,121 bp (GRCm38)
  • T to A, chromosome 8 at 25,793,716 bp (GRCm38)
  • A to G, chromosome 8 at 35,795,483 bp (GRCm38)
  • A to T, chromosome 8 at 69,889,319 bp (GRCm38)
  • T to C, chromosome 9 at 36,713,825 bp (GRCm38)
  • C to T, chromosome 9 at 38,877,743 bp (GRCm38)
  • C to T, chromosome 9 at 82,871,384 bp (GRCm38)
  • A to T, chromosome 9 at 96,686,051 bp (GRCm38)
  • A to T, chromosome 9 at 97,532,710 bp (GRCm38)
  • A to G, chromosome 9 at 106,648,031 bp (GRCm38)
  • A to T, chromosome 9 at 123,183,671 bp (GRCm38)
  • T to A, chromosome 10 at 3,956,822 bp (GRCm38)
  • T to A, chromosome 10 at 57,495,900 bp (GRCm38)
  • T to C, chromosome 10 at 62,432,402 bp (GRCm38)
  • G to A, chromosome 10 at 127,170,711 bp (GRCm38)
  • T to A, chromosome 10 at 130,419,124 bp (GRCm38)
  • A to T, chromosome 11 at 4,491,131 bp (GRCm38)
  • A to T, chromosome 11 at 44,561,509 bp (GRCm38)
  • G to C, chromosome 11 at 55,515,071 bp (GRCm38)
  • G to A, chromosome 11 at 62,860,016 bp (GRCm38)
  • A to G, chromosome 11 at 103,913,883 bp (GRCm38)
  • A to G, chromosome 11 at 120,077,331 bp (GRCm38)
  • T to G, chromosome 12 at 58,985,188 bp (GRCm38)
  • A to T, chromosome 12 at 80,193,643 bp (GRCm38)
  • A to G, chromosome 12 at 103,534,119 bp (GRCm38)
  • T to C, chromosome 13 at 102,789,024 bp (GRCm38)
  • T to A, chromosome 14 at 37,107,386 bp (GRCm38)
  • T to C, chromosome 14 at 51,908,924 bp (GRCm38)
  • A to G, chromosome 14 at 55,704,839 bp (GRCm38)
  • T to C, chromosome 14 at 87,487,260 bp (GRCm38)
  • T to A, chromosome 14 at 103,260,269 bp (GRCm38)
  • A to G, chromosome 15 at 47,698,212 bp (GRCm38)
  • A to G, chromosome 15 at 102,188,667 bp (GRCm38)
  • A to G, chromosome 16 at 18,227,180 bp (GRCm38)
  • A to G, chromosome 16 at 93,690,430 bp (GRCm38)
  • C to T, chromosome 17 at 3,198,010 bp (GRCm38)
  • T to A, chromosome 17 at 13,949,244 bp (GRCm38)
  • T to G, chromosome 17 at 21,722,055 bp (GRCm38)
  • T to A, chromosome 17 at 34,977,996 bp (GRCm38)
  • T to A, chromosome 17 at 47,400,410 bp (GRCm38)
  • A to T, chromosome 18 at 60,390,379 bp (GRCm38)
  • C to T, chromosome 18 at 62,950,119 bp (GRCm38)
  • C to T, chromosome 19 at 4,614,209 bp (GRCm38)
  • T to C, chromosome 19 at 7,865,862 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9520 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069322-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.