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Strain Name:
C57BL/6J-MtgxR9529Btlr/Mmmh
Stock Number:
069330-MU
Citation ID:
RRID:MMRRC_069330-MU
Other Names:
R9529 (G1)
Major Collection:

Strain Information

Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ranbp17
Name: RAN binding protein 17
Synonyms: 4932704E15Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 66011
Homologene: 36409
Sbno1
Name: strawberry notch 1
Synonyms: sno, 9330180L10Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 243272
Homologene: 10055
Clptm1
Name: cleft lip and palate associated transmembrane protein 1
Synonyms: N14, HS9
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 56457
HGNC: HGNC:2087
Homologene: 37464
Zfp106
Name: zinc finger protein 106
Synonyms: Cd-1, H3a, sirm, Sh3bp3
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 20402
Homologene: 40787
Lamb2
Name: laminin, beta 2
Synonyms: Lamb-2, Lams, npht
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 16779
HGNC: HGNC:6487
Homologene: 1723
Cald1
Name: caldesmon 1
Synonyms: 4833423D12Rik, C920027I18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 109624
HGNC: HGNC:1441
Homologene: 137254
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • T to A, chromosome 1 at 4,346,224 bp (GRCm38)
  • T to C, chromosome 1 at 53,522,336 bp (GRCm38)
  • G to A, chromosome 1 at 59,108,056 bp (GRCm38)
  • A to G, chromosome 1 at 150,669,424 bp (GRCm38)
  • A to T, chromosome 2 at 65,764,588 bp (GRCm38)
  • T to C, chromosome 2 at 67,525,196 bp (GRCm38)
  • T to A, chromosome 2 at 112,635,315 bp (GRCm38)
  • A to G, chromosome 2 at 120,520,526 bp (GRCm38)
  • T to C, chromosome 2 at 166,589,598 bp (GRCm38)
  • T to C, chromosome 3 at 64,492,597 bp (GRCm38)
  • T to A, chromosome 3 at 106,211,340 bp (GRCm38)
  • A to T, chromosome 4 at 61,228,118 bp (GRCm38)
  • A to G, chromosome 4 at 123,513,887 bp (GRCm38)
  • T to A, chromosome 4 at 138,311,469 bp (GRCm38)
  • A to G, chromosome 4 at 144,555,512 bp (GRCm38)
  • T to C, chromosome 5 at 86,428,642 bp (GRCm38)
  • T to C, chromosome 5 at 104,974,107 bp (GRCm38)
  • A to G, chromosome 5 at 107,830,572 bp (GRCm38)
  • G to T, chromosome 5 at 109,156,198 bp (GRCm38)
  • A to C, chromosome 5 at 113,183,816 bp (GRCm38)
  • G to A, chromosome 5 at 124,379,350 bp (GRCm38)
  • T to A, chromosome 6 at 34,686,012 bp (GRCm38)
  • T to A, chromosome 6 at 43,167,498 bp (GRCm38)
  • G to T, chromosome 6 at 67,942,296 bp (GRCm38)
  • A to G, chromosome 7 at 19,065,610 bp (GRCm38)
  • T to C, chromosome 7 at 19,637,675 bp (GRCm38)
  • T to C, chromosome 7 at 25,471,806 bp (GRCm38)
  • T to A, chromosome 7 at 90,427,365 bp (GRCm38)
  • T to C, chromosome 7 at 97,961,586 bp (GRCm38)
  • A to T, chromosome 7 at 141,700,884 bp (GRCm38)
  • A to T, chromosome 9 at 20,774,012 bp (GRCm38)
  • G to A, chromosome 9 at 39,550,427 bp (GRCm38)
  • T to C, chromosome 9 at 95,710,197 bp (GRCm38)
  • T to C, chromosome 9 at 102,869,319 bp (GRCm38)
  • T to A, chromosome 9 at 108,486,278 bp (GRCm38)
  • A to T, chromosome 9 at 121,684,794 bp (GRCm38)
  • G to A, chromosome 10 at 43,393,994 bp (GRCm38)
  • A to T, chromosome 10 at 80,894,806 bp (GRCm38)
  • A to T, chromosome 10 at 103,404,722 bp (GRCm38)
  • A to G, chromosome 10 at 116,338,614 bp (GRCm38)
  • A to G, chromosome 10 at 129,322,336 bp (GRCm38)
  • G to A, chromosome 11 at 33,474,826 bp (GRCm38)
  • T to C, chromosome 11 at 67,088,730 bp (GRCm38)
  • A to G, chromosome 11 at 74,084,834 bp (GRCm38)
  • A to G, chromosome 11 at 74,085,770 bp (GRCm38)
  • A to T, chromosome 11 at 110,056,341 bp (GRCm38)
  • A to T, chromosome 11 at 118,268,635 bp (GRCm38)
  • G to A, chromosome 13 at 98,077,265 bp (GRCm38)
  • A to G, chromosome 14 at 14,850,833 bp (GRCm38)
  • T to A, chromosome 14 at 28,475,766 bp (GRCm38)
  • T to C, chromosome 14 at 70,585,139 bp (GRCm38)
  • G to A, chromosome 15 at 98,839,768 bp (GRCm38)
  • C to T, chromosome 16 at 4,764,954 bp (GRCm38)
  • T to A, chromosome 16 at 10,510,776 bp (GRCm38)
  • G to A, chromosome 17 at 28,988,236 bp (GRCm38)
  • A to G, chromosome 18 at 5,213,501 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9529 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069330-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.