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Strain Name:
C57BL/6J-MtgxR9541Btlr/Mmmh
Stock Number:
069340-MU
Citation ID:
RRID:MMRRC_069340-MU
Other Names:
R9541 (G1)
Major Collection:

Strain Information

Hdgfl2
Name: HDGF like 2
Synonyms: HRP-2, Hdgfrp2
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 15193
VEGA: 17
Homologene: 7355
Otx1
Name: orthodenticle homeobox 1
Synonyms: A730044F23Rik, jv
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 18423
HGNC: HGNC:8521
Homologene: 7875
Trib2
Name: tribbles pseudokinase 2
Synonyms: TRB2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 217410
Homologene: 41445
Jarid2
Name: jumonji and AT-rich interaction domain containing 2
Synonyms: Jmj, jumonji
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 16468
HGNC: HGNC:6196
Homologene: 31279
Myo1e
Name: myosin IE
Synonyms: 2310020N23Rik, 9130023P14Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 71602
VEGA: 9
HGNC: HGNC:7599
Homologene: 55864
Mindy3
Name: MINDY lysine 48 deubiquitinase 3
Synonyms: 1810041E18Rik, 5830410F13Rik, 2310047O13Rik, Fam188a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 66960
Homologene: 11478
Pcm1
Name: pericentriolar material 1
Synonyms: 9430077F19Rik, 2600002H09Rik, C030044G17Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18536
HGNC: HGNC:8727
Homologene: 4518
Genetic Alterations
ENU-induced transitions at the following base pair locations:
  • A to T, chromosome 1 at 39,338,113 bp (GRCm38)
  • T to A, chromosome 1 at 75,440,450 bp (GRCm38)
  • G to T, chromosome 1 at 136,627,565 bp (GRCm38)
  • C to A, chromosome 1 at 171,257,126 bp (GRCm38)
  • C to A, chromosome 1 at 191,985,333 bp (GRCm38)
  • T to C, chromosome 2 at 12,386,638 bp (GRCm38)
  • T to C, chromosome 2 at 41,344,588 bp (GRCm38)
  • C to T, chromosome 2 at 76,885,013 bp (GRCm38)
  • T to A, chromosome 2 at 85,438,681 bp (GRCm38)
  • T to A, chromosome 2 at 111,230,930 bp (GRCm38)
  • T to C, chromosome 2 at 119,812,108 bp (GRCm38)
  • T to G, chromosome 3 at 89,091,615 bp (GRCm38)
  • C to A, chromosome 3 at 95,881,215 bp (GRCm38)
  • A to T, chromosome 4 at 88,571,773 bp (GRCm38)
  • G to A, chromosome 4 at 133,578,284 bp (GRCm38)
  • A to G, chromosome 5 at 104,460,061 bp (GRCm38)
  • T to C, chromosome 5 at 115,279,842 bp (GRCm38)
  • A to G, chromosome 5 at 115,616,357 bp (GRCm38)
  • A to G, chromosome 6 at 68,990,941 bp (GRCm38)
  • A to T, chromosome 6 at 112,329,863 bp (GRCm38)
  • A to G, chromosome 6 at 148,998,374 bp (GRCm38)
  • G to A, chromosome 7 at 30,316,918 bp (GRCm38)
  • A to G, chromosome 7 at 44,891,843 bp (GRCm38)
  • T to C, chromosome 7 at 46,151,655 bp (GRCm38)
  • A to G, chromosome 7 at 85,407,001 bp (GRCm38)
  • A to G, chromosome 7 at 135,665,011 bp (GRCm38)
  • A to G, chromosome 8 at 41,327,579 bp (GRCm38)
  • T to C, chromosome 8 at 75,218,572 bp (GRCm38)
  • G to A, chromosome 9 at 45,909,470 bp (GRCm38)
  • A to G, chromosome 9 at 58,860,402 bp (GRCm38)
  • A to T, chromosome 9 at 70,297,346 bp (GRCm38)
  • A to T, chromosome 9 at 70,573,775 bp (GRCm38)
  • G to A, chromosome 9 at 70,605,226 bp (GRCm38)
  • T to A, chromosome 9 at 79,819,853 bp (GRCm38)
  • C to T, chromosome 10 at 41,367,177 bp (GRCm38)
  • A to T, chromosome 10 at 90,577,085 bp (GRCm38)
  • A to G, chromosome 11 at 21,997,052 bp (GRCm38)
  • T to A, chromosome 11 at 76,409,815 bp (GRCm38)
  • G to A, chromosome 11 at 120,367,945 bp (GRCm38)
  • T to A, chromosome 12 at 15,816,826 bp (GRCm38)
  • T to C, chromosome 12 at 81,560,950 bp (GRCm38)
  • C to T, chromosome 13 at 44,914,777 bp (GRCm38)
  • T to C, chromosome 14 at 55,886,652 bp (GRCm38)
  • T to A, chromosome 15 at 78,488,193 bp (GRCm38)
  • G to T, chromosome 16 at 29,422,726 bp (GRCm38)
  • A to G, chromosome 17 at 30,220,464 bp (GRCm38)
  • C to T, chromosome 17 at 37,111,932 bp (GRCm38)
  • T to C, chromosome 17 at 46,438,158 bp (GRCm38)
  • T to A, chromosome 17 at 56,098,976 bp (GRCm38)
  • G to A, chromosome 18 at 36,624,644 bp (GRCm38)
  • C to T, chromosome 19 at 5,039,868 bp (GRCm38)
  • T to G, chromosome Y at 910,801 bp (GRCm38)
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R9541 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069340-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.